| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,192,822 | T→A | 14.3% | intergenic (+155/‑47) | PP_4572 → / → PP_4573 | hypothetical protein/AAA family ATPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,192,822 | 0 | T | A | 14.3% | 86.2 / 9.2 | 35 | intergenic (+155/‑47) | PP_4572/PP_4573 | hypothetical protein/AAA family ATPase |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base A (3/2); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-02 | |||||||||||
GACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGG > NC_002947/5192676‑5192966 | gACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAGCCTGACAGCTTGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAc < 2:401573/150‑1 (MQ=255) aGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAAACCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGc > 1:230716/1‑150 (MQ=255) aCCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGccc < 1:192108/150‑1 (MQ=255) ccGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAccc < 2:60828/127‑1 (MQ=255) ccGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTAccc > 1:60828/1‑127 (MQ=255) tGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACCAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCTCGGTTAACCCCGCCCCTACAACCTTATTAGGCGCCCGTTTACCCGCGCAGAGGCCCGCAAAGCCCTAAACaga < 2:35307/150‑1 (MQ=255) cGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAgg > 2:664216/1‑150 (MQ=255) tttGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGAc > 2:576901/1‑150 (MQ=255) cgcgTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCg > 2:305743/1‑150 (MQ=255) gAAACAACTCCAGATTTTTTTCTTCCAGCTGAGGCCGGTTCGCGGGTAAACCCGCCCCTACAACAATCTCCGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGa < 1:352563/150‑1 (MQ=255) aTCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGa > 2:690462/1‑111 (MQ=255) aTCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGa < 1:690462/111‑1 (MQ=255) tCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCg > 1:86293/1‑150 (MQ=255) tgctgcCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGAc < 1:305743/150‑1 (MQ=255) tGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAAc > 1:128643/1‑150 (MQ=255) gggCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCg < 2:128643/150‑1 (MQ=255) ctctTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTaa > 2:285550/1‑150 (MQ=255) ggTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCg < 1:6079/137‑1 (MQ=255) ggTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCg > 2:6079/1‑137 (MQ=255) aCCCGCCCCTACAACCTTATTAGGCCCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTAcc < 1:285550/150‑1 (MQ=255) ccGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCt > 2:465147/1‑150 (MQ=255) gCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACg < 2:27990/102‑1 (MQ=255) gCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACg > 1:27990/1‑102 (MQ=255) cccTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGaa > 2:43519/1‑150 (MQ=255) aaCCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTCCACTCGAACGCCCg > 2:388548/1‑150 (MQ=255) cTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTg > 1:165979/1‑150 (MQ=255) ttaGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTgg < 2:464773/150‑1 (MQ=255) ctcttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGcc > 2:415523/6‑141 (MQ=255) ctcttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGcc < 1:415523/136‑1 (MQ=255) cttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTc > 2:201916/4‑150 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGaaa < 2:564013/102‑1 (MQ=255) ttcgcgGGTAAACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGaaa > 1:564013/3‑104 (MQ=255) gcgcGTGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAANCTGGCGNTAAACGCGGCCANTACCCTCGAACGCCCGCTGTTGGTCaa < 1:388548/150‑1 (MQ=255) gcgcGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCaa < 2:557055/150‑1 (MQ=255) cgGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAggg < 1:465147/150‑1 (MQ=255) | GACGGGGCATTCAGGGACTGAATACCCGGTCTGCGTTTGCAGCCGCCAGGCCCCGCGTTATAGTCGGAAACAAATCCAGATCATGTGCTGCCTGTTCGGGCCTCTTCGCGGGTAAACCCGCCCCTACAACCTTATTAGGCGCGGGTTTACCCGCGAAGAGGCCCGCAAAGCCCTAAACAGAGGACAAACCCCCATGAAGTTCGAAGGCACCCGCGACTACGTCGCCACAGATGACCTGAAACTGGCGGTAAACGCGGCCATTACCCTCGAACGCCCGCTGTTGGTCAAGGG > NC_002947/5192676‑5192966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |