| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,233,594 | A→T | 12.5% | D137V (GAC→GTC) | PP_4611 → | ECF family RNA polymerase sigma‑70 factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,233,594 | 0 | A | T | 12.5% | 108.2 / 7.6 | 40 | D137V (GAC→GTC) | PP_4611 | ECF family RNA polymerase sigma‑70 factor |
| Reads supporting (aligned to +/- strand): ref base A (21/14); new base T (2/3); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CTACCTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCA > NC_002947/5233445‑5233737 | cTACCTGCAAGCGCGCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGa < 2:41781/150‑1 (MQ=255) gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCa < 2:560183/150‑1 (MQ=255) tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg > 2:726815/1‑150 (MQ=255) tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg > 2:377635/1‑150 (MQ=255) cGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCagca < 2:180277/150‑4 (MQ=255) acaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGa > 2:284164/1‑150 (MQ=255) gTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtc > 1:311816/1‑150 (MQ=255) ggAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt > 2:311514/1‑144 (MQ=255) ggAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt < 1:311514/144‑1 (MQ=255) gAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgctcgc > 1:297459/1‑150 (MQ=255) tCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTgg > 1:322891/1‑150 (MQ=255) gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGGTGGTCAAACGCGCCTTTGTGTTCGCCGATTTGGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGc > 1:167784/1‑150 (MQ=255) ggAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGc > 1:517440/1‑150 (MQ=255) aaGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg < 1:686125/110‑1 (MQ=255) aaGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg > 2:686125/1‑110 (MQ=255) ggCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCaa < 1:377635/150‑1 (MQ=255) gCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAg < 2:311816/150‑1 (MQ=255) tcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAcc > 2:264630/1‑150 (MQ=255) gagaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCa > 1:36674/1‑92 (MQ=39) gagaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCa < 2:36674/92‑1 (MQ=255) ttGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCa < 2:297459/150‑1 (MQ=255) gCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc < 1:284164/150‑1 (MQ=255) gACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc < 2:167784/150‑1 (MQ=255) cACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTAc > 1:340658/1‑150 (MQ=255) ggCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACt > 1:381718/1‑80 (MQ=255) ggCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACt < 2:381718/80‑1 (MQ=255) gCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa < 1:726815/150‑1 (MQ=255) cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc > 1:138557/1‑132 (MQ=255) cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc < 2:138557/132‑1 (MQ=255) tCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGcc < 1:120102/150‑1 (MQ=255) ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt > 2:32758/1‑142 (MQ=255) ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt < 1:32758/142‑1 (MQ=255) ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGc < 1:57662/148‑1 (MQ=255) ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGc > 2:57662/1‑148 (MQ=255) aCCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAAGAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCg > 1:347710/1‑150 (MQ=255) ttCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCgg > 2:565839/1‑150 (MQ=255) cttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTGCCTGAACAAGGCAGCCATGCgctgc < 1:517715/101‑1 (MQ=39) cttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTGCCTGAACAAGGCAGCCATGCgctgc > 2:517715/3‑103 (MQ=38) ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTg > 2:16750/1‑150 (MQ=255) ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTg > 2:550155/1‑150 (MQ=255) aGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCa > 2:57846/1‑150 (MQ=255) | CTACCTGCAAGCGCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCA > NC_002947/5233445‑5233737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |