Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,233,597 G→A 12.1% G138D (GGC→GAC)  PP_4611 → ECF family RNA polymerase sigma‑70 factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,233,5970GA12.1% 99.5 / 3.1 40G138D (GGC→GAC) PP_4611ECF family RNA polymerase sigma‑70 factor
Reads supporting (aligned to +/- strand):  ref base G (21/14);  new base A (2/3);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGTTGAT  >  NC_002947/5233457‑5233746
                                                                                                                                            |                                                                                                                                                     
gcTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCa                                                                                                                                              <  2:560183/150‑1 (MQ=255)
  tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg                                                                                                                                            >  2:726815/1‑150 (MQ=255)
  tCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAg                                                                                                                                            >  2:377635/1‑150 (MQ=255)
   cGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCagca                                                                                                                                           <  2:180277/150‑4 (MQ=255)
      acaGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGa                                                                                                                                        >  2:284164/1‑150 (MQ=255)
          gTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtc                                                                                                                                    >  1:311816/1‑150 (MQ=255)
               ggAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt                                                                                                                                     >  2:311514/1‑144 (MQ=255)
               ggAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAt                                                                                                                                     <  1:311514/144‑1 (MQ=255)
                gAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAAtcgctcgc                                                                                                                              >  1:297459/1‑150 (MQ=255)
                       tCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTgg                                                                                                                       >  1:322891/1‑150 (MQ=255)
                         gcgcCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGGTGGTCAAACGCGCCTTTGTGTTCGCCGATTTGGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGc                                                                                                                     >  1:167784/1‑150 (MQ=255)
                                    ggAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGc                                                                                                          >  1:517440/1‑150 (MQ=255)
                                           aaGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg                                                                                                                                           >  2:686125/1‑110 (MQ=255)
                                           aaGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAgg                                                                                                                                           <  1:686125/110‑1 (MQ=255)
                                             ggCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCaa                                                                                                 <  1:377635/150‑1 (MQ=255)
                                              gCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAg                                                                                                <  2:311816/150‑1 (MQ=255)
                                                     tcTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTAcc                                                                                         >  2:264630/1‑150 (MQ=255)
                                                          gagaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCa                                                                                                                                              >  1:36674/1‑92 (MQ=39)
                                                          gagaCATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGCCGTCGACCTGGGCCa                                                                                                                                              <  2:36674/92‑1 (MQ=255)
                                                                ttGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCa                                                                              <  2:297459/150‑1 (MQ=255)
                                                                  gCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGc                                                                            <  1:284164/150‑1 (MQ=255)
                                                                            gACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc                                                                  <  2:167784/150‑1 (MQ=255)
                                                                               cACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTAc                                                               >  1:340658/1‑150 (MQ=255)
                                                                                           ggCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACt                                                                                                                         >  1:381718/1‑80 (MQ=255)
                                                                                           ggCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACt                                                                                                                         <  2:381718/80‑1 (MQ=255)
                                                                                            gCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGaa                                                  <  1:726815/150‑1 (MQ=255)
                                                                                              cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc                                                                  >  1:138557/1‑132 (MQ=255)
                                                                                              cTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCgctgc                                                                  <  2:138557/132‑1 (MQ=255)
                                                                                                tCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGcc                                              <  1:120102/150‑1 (MQ=255)
                                                                                                 ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                                     >  2:32758/1‑142 (MQ=255)
                                                                                                 ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCtgt                                                     <  1:32758/142‑1 (MQ=255)
                                                                                                 ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGc                                               <  1:57662/148‑1 (MQ=255)
                                                                                                 ccACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGc                                               >  2:57662/1‑148 (MQ=255)
                                                                                                   aCCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAAGAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCg                                           >  1:347710/1‑150 (MQ=255)
                                                                                                                      ttCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCgg                        >  2:565839/1‑150 (MQ=255)
                                                                                                                           cttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTGCCTGAACAAGGCAGCCATGCgctgc                                                                  <  1:517715/101‑1 (MQ=39)
                                                                                                                           cttgGCCCAGGCCGTCGACCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTGCCTGAACAAGGCAGCCATGCgctgc                                                                  >  2:517715/3‑103 (MQ=38)
                                                                                                                                 ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTg             >  2:16750/1‑150 (MQ=255)
                                                                                                                                 ccAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTg             >  2:550155/1‑150 (MQ=255)
                                                                                                                                   aGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCa           >  2:57846/1‑150 (MQ=255)
                                                                                                                                            gCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGTTGAt  <  2:517440/150‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                     
GCTCGCACAGGTCCCGGAAGAGGTCGCGCCGTGCGAGGAACAGAAGGCGATCATCTTCGAGACATTGCTAGCGCTCGACCACTTGCTGGACGGCCTTCCACCGTTGGTCAAACGCGCCTTCCTGCTGGCCCAGGTCGACGGCCTGGGCCAAGGCGAAATCGCTCGCGAACTGGGCATTTCGCTGGCCACGGTCAAGCGCTACCTGAACAAGGCAGCCATGCGCTGCTACTTCGCGCTGTGAACGCCTCGTTTTCCCCGCTGGTGGCGGAACAGGCCGTGCATTGGTTGAT  >  NC_002947/5233457‑5233746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑