Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,261,125 A→C 10.0% L175R (CTG→CGG)  PP_4637 ← 5‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,261,1250AC10.0% 106.3 / 4.8 40L175R (CTG→CGG) PP_46375‑methyltetrahydropteroyltriglutamate‑ homocysteine S‑methyltransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (19/17);  new base C (2/2);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCA  >  NC_002947/5260981‑5261271
                                                                                                                                                |                                                                                                                                                  
aGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGAGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTg                                                                                                                                               >  2:338597/1‑150 (MQ=255)
           cAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCGCCAACCTGCCGGTTgatggcct                                                                                                                                        <  2:593506/146‑8 (MQ=255)
           cAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCGCCAACCTGCCGGTTgatggcct                                                                                                                                        >  1:593506/1‑139 (MQ=255)
                  caAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTg                                                                                                                             >  1:662384/1‑150 (MQ=255)
                    aggcgaggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGAGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGcc                                                                                                                           <  1:338597/150‑1 (MQ=255)
                         gggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGTATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCa                                                                                                                      <  2:552128/149‑1 (MQ=255)
                          ggcgTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGCTTGCCTTCCAg                                                                                                                     <  2:386798/150‑1 (MQ=255)
                                     cGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGt                                                                                                          >  2:279047/1‑150 (MQ=255)
                                         gACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGt                                                                                                      <  1:723133/150‑1 (MQ=255)
                                          aCCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTg                                                                                                     >  1:649473/1‑150 (MQ=255)
                                            cAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgat                                                                                                                                             >  1:118930/1‑106 (MQ=255)
                                            cAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCTGCCGGTTgat                                                                                                                                             <  2:118930/108‑3 (MQ=255)
                                                 ccAGCGCCAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCTCCGAAGTACGTGGCGATCa                                                                                              <  2:85821/150‑1 (MQ=255)
                                                  cAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAg                                                                                             <  1:279047/150‑1 (MQ=255)
                                                         aGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCttcttc                                                                                      <  2:684895/150‑1 (MQ=255)
                                                                         gCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGaa                                                                      <  2:119549/150‑1 (MQ=255)
                                                                                aTCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTggcg                                                                                                                                            <  2:463495/73‑1 (MQ=255)
                                                                                aTCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTggcg                                                                                                                                            >  1:463495/1‑73 (MQ=255)
                                                                                   gagaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTa                                                            >  2:715917/1‑150 (MQ=255)
                                                                                     gaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGc                                                                                            <  1:377065/116‑1 (MQ=255)
                                                                                     gaATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGc                                                                                            >  2:377065/1‑116 (MQ=255)
                                                                                         ggTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGAcgcg                                                      >  2:345658/1‑150 (MQ=255)
                                                                                            cTGGTACTGCTCGGGCGCCTGAACAAGATCGACGTGCAGGCGATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTc                                                   >  2:572993/1‑150 (MQ=255)
                                                                                             tGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCa                                                  <  1:715917/150‑1 (MQ=255)
                                                                                               gTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGaa                                                                                                            >  2:157592/1‑90 (MQ=255)
                                                                                               gTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGaa                                                                                                            <  1:157592/90‑1 (MQ=255)
                                                                                               gTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATa                                                <  2:662384/150‑1 (MQ=255)
                                                                                                              cTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTc                                 >  1:334155/1‑150 (MQ=255)
                                                                                                               tGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTcc                                >  2:107689/1‑150 (MQ=255)
                                                                                                                   ccAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTggg                            >  2:548092/1‑150 (MQ=255)
                                                                                                                             gTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTg                  <  1:362653/150‑1 (MQ=255)
                                                                                                                               gCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGGAGTACGTGGCGATCAGCGTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTggg                >  2:203443/1‑150 (MQ=255)
                                                                                                                                 aGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCATGGGCg              >  1:575359/1‑150 (MQ=255)
                                                                                                                                  ggCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCATGAGccc             <  2:575359/150‑3 (MQ=255)
                                                                                                                                   gCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAtgttg                                                              <  2:443533/100‑1 (MQ=255)
                                                                                                                                   gCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGAtgttg                                                              >  1:443533/1‑100 (MQ=255)
                                                                                                                                   gCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAg            <  1:548092/150‑1 (MQ=255)
                                                                                                                                     cATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGCTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGa          >  2:618560/1‑150 (MQ=255)
                                                                                                                                           ccGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCAACGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGtt    >  2:339112/1‑150 (MQ=255)
                                                                                                                                             ggCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGCCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCa  >  1:160977/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AGGGTCTTGCCCAAATCACAAGGCGAGGCGTTGCGACCGTCGACCAGGCCCAGCGACAGTACCTTGTAGGCCGGCAGGCGATCGAGAATGGTCTGGTACTGCTCGGGCGCCTGAACCAGATCGACGTGCAGGCCATCAACCGGCAGGTTGGCGGCCAGGCCGAGGTTGCCTTCCAGGCCACCGAAGTACGTGGCGATCAGCTTCTTCAACGGTGCGCGTTGAAGGATGTTGTAGACGCGTTCATAGGCGTTTTTCCAGTCCTGGGGCAGCGCCTGGGCGAGGATGGGTTCA  >  NC_002947/5260981‑5261271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑