Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,364,531 C→T 8.6% A51T (GCC→ACC)  folP ← dihydropteroate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,364,5310CT 8.6% 125.4 / 2.9 47A51T (GCC→ACC) folPdihydropteroate synthase
Reads supporting (aligned to +/- strand):  ref base C (20/22);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01

ACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGG  >  NC_002947/5364386‑5364663
                                                                                                                                                 |                                                                                                                                    
aCCACGTCGAGGCGCCTGTTGATGGCCTCCACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTgg                                                                                                                                  <  2:101261/150‑1 (MQ=255)
  cACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCAGCGGCAACCATCGCTTCGGCGTGgcg                                                                                                                                >  2:616766/1‑150 (MQ=255)
      tCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAgg                                                                                                                            <  2:655382/150‑1 (MQ=255)
           gCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCtc                                                                                                                       <  2:621773/150‑1 (MQ=255)
                tGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgc                                                                                                                  >  1:745100/1‑150 (MQ=255)
                tGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGAGCAGAAGGTAGGCATCGCTTCGGCGTGGCGCAGGGCCTCGTcgc                                                                                                                  >  2:552355/1‑150 (MQ=255)
                     gtGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGGGGACTCGCCACCGAGATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                                                             <  2:280923/149‑1 (MQ=255)
                          cTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCACACCCGGGCGCGTGGACTCGCCACCGATATAAATCAACGTTGCACCGGCGGCAACCAACGCATCAACGTAGAGCAAGACATCGTCGAGcttaaagaa                                                                                                        >  2:319770/1‑143 (MQ=255)
                                  ttGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGc                                                                                                <  1:161448/150‑1 (MQ=255)
                                     gcgcCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCAt                                                                                             >  1:214476/1‑150 (MQ=255)
                                         cacaCGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGga                                                                                         <  2:214476/150‑1 (MQ=255)
                                                 ccAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGCCGTGCTGACTGAAGCGACCGCCATCGGAGAAGGAAt                                                                                 <  1:552355/150‑1 (MQ=255)
                                                  cAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATc                                                                                >  1:328462/1‑150 (MQ=255)
                                                  cAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATc                                                                                <  2:745100/150‑1 (MQ=255)
                                                            gTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGata                                                                      <  1:648528/150‑1 (MQ=255)
                                                                 cGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGGGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgag                                                                 <  2:363131/150‑1 (MQ=255)
                                                                  gagaCTGCCCGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCAGCGGCAACCATCGCTTAGGAGTGTCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTgaga                                                                <  1:616766/150‑1 (MQ=255)
                                                                            gcgcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGa                                                      <  2:732452/150‑1 (MQ=255)
                                                                              gcACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACa                                                    >  2:68036/1‑150 (MQ=255)
                                                                                aCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATg                                                  >  1:156085/1‑150 (MQ=255)
                                                                                aCCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATg                                                  >  2:546987/1‑150 (MQ=255)
                                                                                          gTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGAc                                        >  1:496191/1‑150 (MQ=255)
                                                                                              aCTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATCACCTGGGTATGcaacaaca                                    >  2:568907/1‑148 (MQ=255)
                                                                                               cTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATc                                   <  1:68036/150‑1 (MQ=255)
                                                                                                tCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCa                                  >  1:690501/1‑150 (MQ=255)
                                                                                                    cACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                                                             >  2:443715/1‑71 (MQ=255)
                                                                                                    cACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                                                             <  1:443715/71‑1 (MQ=255)
                                                                                                      ccGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                                                                           <  1:311396/103‑1 (MQ=255)
                                                                                                      ccGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCggggg                                                                           >  2:311396/1‑103 (MQ=255)
                                                                                                              gATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGACTCGTCGCGCTGACTGaa                                                                                                        >  1:104808/1‑66 (MQ=255)
                                                                                                              gATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGACTCGTCGCGCTGACTGaa                                                                                                        <  2:104808/66‑1 (MQ=255)
                                                                                                               aTCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCg                   >  1:386473/1‑150 (MQ=255)
                                                                                                                tCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                                                             <  2:325977/59‑1 (MQ=255)
                                                                                                                tCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGctga                                                                                                             >  1:325977/1‑59 (MQ=255)
                                                                                                                  aaCGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGCGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCaa                <  2:156085/150‑1 (MQ=255)
                                                                                                                   aCGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAg               >  2:310534/1‑150 (MQ=255)
                                                                                                                   aCGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCAATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAg               <  1:494633/150‑1 (MQ=255)
                                                                                                                            cggcggCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCggg      <  1:310534/150‑1 (MQ=255)
                                                                                                                                ggcAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCgggtagg  <  2:328462/150‑1 (MQ=255)
                                                                                                                                     ccATCGCTTCGGTGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa                                                         >  2:350047/1‑90 (MQ=255)
                                                                                                                                     ccATCGCTTCGGTGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa                                                         >  2:350024/1‑90 (MQ=255)
                                                                                                                                     ccATCGCTTCGGTGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa                                                         <  1:350047/90‑1 (MQ=255)
                                                                                                                                     ccATCGCTTCGGTGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCa                                                         <  1:350024/90‑1 (MQ=255)
                                                                                                                                        tCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATGCCCATGACATgg                                                 <  2:217039/95‑1 (MQ=255)
                                                                                                                                        tCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATGCCCATGACATgg                                                 >  1:217039/1‑95 (MQ=255)
                                                                                                                                            ttCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt                                                        <  2:56676/84‑1 (MQ=255)
                                                                                                                                            ttCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCAt                                                        >  1:56676/1‑84 (MQ=255)
                                                                                                                                                 |                                                                                                                                    
ACCACGTCGAGGCGACTGTTGATGGCCTCGACCATTGGCGCCACACGCTCCAGCTCCTCGGTCACCGAGACTGCACGCGCACCCGGGCGCGTGGACTCGCCACCGATATCGATCAACGTTGCGCCGGCGGCAACCATCGCTTCGGCGTGGCGCAGGGCCTCGTCGCGCTGACTGAAGCGACCGCCATCGGAGAAGGAATCGGGGGTGATATTGAGAATACCCATGACATGGGTATGCGACAAATCAAGAACCCGGCTGCCGCAAGGCAACCGGGTAGG  >  NC_002947/5364386‑5364663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑