Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,390,704 G→C 7.9% noncoding (24/87 nt) PP_mr56 → C4 ncRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,390,7040GC 7.9% 140.6 / 3.4 51noncoding (24/87 nt)PP_mr56C4 ncRNA
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base C (2/2);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

TACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCA  >  NC_002947/5390567‑5390848
                                                                                                                                          |                                                                                                                                                
tACTGCCTGCGCCTGTTTTCCGTTGCGTTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTtgcgt                                                                                                                                      <  2:413564/150‑1 (MQ=255)
     cctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGcc                                                                                                                                 >  1:530467/1‑150 (MQ=255)
     cctgcGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTCTAGTCTCGGTGGGCCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGGTCTTGTATCG‑ACATTACCGTTCATGGCGGTTGTGCATTGGGCACGCTTGCGTGCGcc                                                                                                                                 >  2:19544/1‑150 (MQ=255)
        gcgcCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGt                                                                                                                              >  1:678874/1‑150 (MQ=255)
            cTGTTTTCCGTTGCGGTCATTTTTTTAGTGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                                                                                          >  2:399550/1‑150 (MQ=255)
            cTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCt                                                                                                                          <  1:660668/150‑1 (MQ=255)
             tGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTtgc                                                                                                                                        >  1:156102/1‑135 (MQ=255)
             tGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTtgc                                                                                                                                        <  2:156102/135‑1 (MQ=255)
               ttttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTGGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGATGTGCATACGACACGCTTGCGTGCGCCGGTTTCtgtt                                                                                                                       >  2:162536/1‑149 (MQ=255)
               ttttCCGTTGCGGTCATTTTTTCAGGGTTCTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCCTGTACCACGCTTGCGTGCGCCGGTTTCtgtg                                                                                                                       <  1:19544/150‑1 (MQ=255)
                tttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTgtgt                                                                                                                      <  2:540645/150‑1 (MQ=255)
                 ttCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGtt                                                                                                                     >  2:654842/1‑150 (MQ=255)
                      ttGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCg                                                                                                                >  2:118467/1‑150 (MQ=255)
                         ccgTCATTTTTTCCGGGTTTTAGTCTCGGTGGGTCGTTCCTCATCAACGACCGGCCTTGACAGGCCGCAACGAC‑ACACAGCGCGCATAACCGTTCCTGGCGGTTGTGCATGGGGCACGCCCGCGTGCTCCGGCTTCTGTGTtcgtcgctg                                                                                                             <  2:217861/148‑4 (MQ=255)
                          ggTCATTTTTTCAGGGTGTTCGTCTCGGTGGCTCTTTTCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCt                                                                                                            <  1:344164/150‑1 (MQ=255)
                                       gggTTTTAGTCTCGGTGGGTCGTTGCTCATCAAAAACCGGCGGTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGca                                                                                               <  2:740690/150‑1 (MQ=255)
                                        ggTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGcac                                                                                              <  1:489874/150‑1 (MQ=255)
                                            ttAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCt                                                                                          <  2:450554/150‑1 (MQ=255)
                                                tctcGGTGGGTCGTTGGTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCATGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGcca                                                                                      >  1:707892/1‑150 (MQ=255)
                                                tctcGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCt                                                                                          >  2:376091/1‑146 (MQ=255)
                                                tctcGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCt                                                                                          <  1:376091/146‑1 (MQ=255)
                                                        ggTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCg                                                                              <  2:437836/150‑1 (MQ=255)
                                                         gTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGt                                                                             <  2:678874/150‑1 (MQ=255)
                                                                cTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAAGCGTGccccat                                                                                                                               <  2:574766/93‑3 (MQ=38)
                                                                cTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAAGCGTGccccat                                                                                                                               >  1:574766/1‑91 (MQ=38)
                                                                  catcaACGACCGGCTTTCACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCg                                                                    >  2:384874/1‑150 (MQ=255)
                                                                   atcaACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCgg                                                                   >  1:224355/1‑150 (MQ=255)
                                                                         gACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGc                                                             <  1:399550/150‑1 (MQ=255)
                                                                            cGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGtt                                                                            >  1:434470/1‑132 (MQ=255)
                                                                            cGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGtt                                                                            <  2:434470/132‑1 (MQ=255)
                                                                            cGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGt                                                          >  2:15889/1‑150 (MQ=255)
                                                                                   gACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAagcgtgccc                                                                                                                                  <  1:355892/71‑3 (MQ=255)
                                                                                   gACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGCGCACGCAagcgtgccc                                                                                                                                  >  2:355892/1‑69 (MQ=255)
                                                                                     cAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTAt                                                 <  1:270414/150‑1 (MQ=255)
                                                                                        gCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCga                                              <  1:255889/150‑1 (MQ=255)
                                                                                          cGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCgaga                                            <  2:224355/150‑1 (MQ=255)
                                                                                           gCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTg                                                               <  2:345471/130‑1 (MQ=255)
                                                                                           gCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTg                                                               >  1:345471/1‑130 (MQ=255)
                                                                                            cAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAac                                          <  1:118467/150‑1 (MQ=255)
                                                                                                         gcgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGc                              >  1:503901/1‑150 (MQ=255)
                                                                                                         gcgcgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAACTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGc                              >  1:344192/1‑150 (MQ=255)
                                                                                                            cgcATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTaa                           <  1:15889/150‑1 (MQ=255)
                                                                                                               aTAATCGTTCCTGGCGGTTGTGCATGGGGGACGCTTGCGTGCTCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGa                        <  2:607588/150‑1 (MQ=255)
                                                                                                                  aCCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCAATTTACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCg                     <  1:654842/150‑1 (MQ=255)
                                                                                                                   ccGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTcc                                                                               <  1:68735/91‑1 (MQ=255)
                                                                                                                   ccGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTcc                                                                               >  2:68735/1‑91 (MQ=255)
                                                                                                                    cGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCAGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGtt                   <  1:384874/150‑1 (MQ=255)
                                                                                                                      ttCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATTCTAAAGATCGTTcc                 <  1:162536/150‑1 (MQ=255)
                                                                                                                                  gtgCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTca     >  2:529861/1‑150 (MQ=255)
                                                                                                                                  gtgCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTca     >  1:392892/1‑150 (MQ=255)
                                                                                                                                     cATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTcacca  >  1:3753/1‑150 (MQ=255)
                                                                                                                                          |                                                                                                                                                
TACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCGTTGCTCATCAACGACCGGCTTTGACAGGCCGCAACGAT‑ACACAGCGCGCATAACCGTTCATGGCGGTTGTGCATGGGGCACGCTTGCGTGCGCCGGTTTCTGTGTTCGCCGGTCTGTCAACCCATGCACAGCTGCCACCATTCCGTTTGACAGCGGGTTGGCGGTGGCCCCTATCGAGAACACAGTAAAATGCTAAAGATCGTTCCTGACCCGCCTCACCA  >  NC_002947/5390567‑5390848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑