Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,343 T→C 15.2% N270D (AAT→GAT)  rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,3430TC15.2% 102.8 / 9.5 46N270D (AAT→GAT) rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (18/20);  new base C (4/3);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCAC  >  NC_002947/5427194‑5427475
                                                                                                                                                     |                                                                                                                                    
gAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCAtt                                                                                                                                      <  1:625516/150‑1 (MQ=255)
           ccAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCCCAAGACATTACACCTCAGGGGTCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCgg                                                                                                                           >  1:655546/1‑150 (MQ=255)
            cAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                          >  1:95807/1‑150 (MQ=255)
            cAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGATCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTCCGGc                                                                                                                          >  2:545990/1‑150 (MQ=255)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCTACCAGCACTTGCACCGCACGGGCCTAGATCCTTTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCa                                                                                                                        <  1:731149/150‑1 (MQ=39)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCa                                                                                                                        <  2:201304/150‑1 (MQ=255)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACggt                                                                                                                                <  2:419897/142‑3 (MQ=255)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACggt                                                                                                                                >  1:420038/1‑140 (MQ=255)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACggt                                                                                                                                >  1:419897/1‑140 (MQ=255)
              gCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACggt                                                                                                                                <  2:420038/142‑3 (MQ=255)
                      tCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAgcgc                                                                                                                >  1:58022/1‑150 (MQ=255)
                        aTCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCCGTGGGCGCTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTc                                                                                                              >  2:430773/1‑150 (MQ=255)
                         tCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTcc                                                                                                             >  1:463029/1‑150 (MQ=255)
                               tCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGc                                                                                                       <  2:548027/150‑1 (MQ=255)
                                 cAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTg                                                                                                     >  2:171621/1‑150 (MQ=255)
                                   ggCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGAt                                                                                                   <  2:456456/150‑1 (MQ=255)
                                      cACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                             <  1:535037/121‑1 (MQ=255)
                                      cACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTc                                                                                                                             >  2:535037/1‑121 (MQ=255)
                                      cACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCgg                                                                                                >  1:61275/1‑149 (MQ=255)
                                      cACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGa                                                                                                >  1:589757/1‑150 (MQ=255)
                                       aCGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTTTCTGCTGATGTGTGGGACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGAc                                                                                               <  1:130474/150‑1 (MQ=255)
                                                        tCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTgg                                                                              <  2:95807/150‑1 (MQ=255)
                                                         ggCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCTGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGc                                                                             <  1:329783/149‑1 (MQ=255)
                                                           ccaccaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGa                                                                           >  2:568207/1‑150 (MQ=255)
                                                             accaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACg                                                                                                                                  >  2:328438/1‑93 (MQ=255)
                                                             accaGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGAATGCCGACGGCATCCACg                                                                                                                                  <  1:328438/93‑1 (MQ=255)
                                                                 gCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCCAGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCa                                                                     <  1:603437/150‑1 (MQ=255)
                                                                  cACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                          >  2:21950/1‑96 (MQ=255)
                                                                  cACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGc                                                                                                                          <  1:21950/96‑1 (MQ=255)
                                                                     ttGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCAc                                                                 >  1:226321/1‑150 (MQ=255)
                                                                                gggCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGAc                                                                                               <  1:535555/109‑1 (MQ=255)
                                                                                gggCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGAc                                                                                               >  2:535555/1‑109 (MQ=255)
                                                                                  gCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAgcgc                                                    <  2:61275/150‑1 (MQ=255)
                                                                                     ttGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCgctgc                                                 <  1:171621/150‑1 (MQ=255)
                                                                                              tCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAgcg                                                     >  2:515197/1‑137 (MQ=255)
                                                                                              tCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAgcg                                                     <  1:515197/137‑1 (MQ=255)
                                                                                                  gcgcACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGt                                    <  2:58022/150‑1 (MQ=255)
                                                                                                       cGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAg                               <  2:752501/150‑1 (MQ=255)
                                                                                                            gCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTc                                                          >  1:360912/1‑118 (MQ=255)
                                                                                                            gCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTATTGGCGAACACCAGCACCTGCTTc                                                          <  2:360912/118‑1 (MQ=255)
                                                                                                             cGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAgg                         >  2:636698/1‑150 (MQ=255)
                                                                                                                   gcgtgtgtgCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGcc                   <  1:85126/147‑1 (MQ=255)
                                                                                                                     cTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCtt                 <  2:655546/150‑1 (MQ=255)
                                                                                                                         gggCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTgcgc             >  1:268900/1‑150 (MQ=255)
                                                                                                                                  ccGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTcc    <  1:568207/150‑1 (MQ=255)
                                                                                                                                    gTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcac  <  2:463029/150‑1 (MQ=255)
                                                                                                                                    gTGAATGCCGACGGCATCCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCcac  >  2:605663/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                    
GAGGTTGACGACCAGCGGCAAGTCATCGATATCCAGGCCACGGGCCGCCACATCGGTCGCCACCAGCACTTGCACCTCACGGGCCTTGAAGCTGTCCAGCGCACGCTGGCGGGTTGCCTGTGGGCGGTCACCGTGGATGCCGTCGGCATTCACGCCTTCGGCCAGCAAGCGCTCCACCAGCTGATCGACGCCATTGCGGGTCTTGGCGAACACCAGCACCTGCTTCCAGCGCTGCTTGCGCAGCAGGTGGCAGAACAGGTCAGCCTTGCGCTTCTTGTCCAC  >  NC_002947/5427194‑5427475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑