| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,506,680 | G→A | 10.5% | intergenic (+91/‑108) | PP_4839 → / → cycA | iron‑regulated membrane protein/D‑alanine/beta‑alanine/D‑serine/glycine permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,506,680 | 0 | G | A | 10.5% | 101.8 / 3.7 | 38 | intergenic (+91/‑108) | PP_4839/cycA | iron‑regulated membrane protein/D‑alanine/beta‑alanine/D‑serine/glycine permease |
| Reads supporting (aligned to +/- strand): ref base G (20/14); new base A (2/2); total (22/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01 | |||||||||||
GTGGATAGTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCATAGCGACAGATGAACCGCAC > NC_002947/5506542‑5506826 | gTGGATAGTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACGCGGTCC‑C‑TCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg > 1:198488/1‑146 (MQ=11)gTGGATAGTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTACCGGCCTCTTCGCGGGTAAACCCGCTCCTACAACG‑CGGTC‑CCTCGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAgg < 2:198488/146‑1 (MQ=11) tAGTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCt < 1:553586/150‑1 (MQ=255) gTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGa < 1:647654/150‑1 (MQ=255) gcgcCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCag > 1:289590/1‑150 (MQ=255) ttGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCt > 1:555193/1‑150 (MQ=255) aGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAg < 2:32904/98‑1 (MQ=255) aGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAg > 1:32904/1‑98 (MQ=255) ccTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAAc > 2:575058/1‑150 (MQ=255) cTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCa > 2:264382/1‑119 (MQ=255) cTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCa < 1:264382/119‑1 (MQ=255) gCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCaga > 2:627728/1‑113 (MQ=255) gCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCaga < 1:627728/113‑1 (MQ=255) cTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTtag > 1:616005/1‑150 (MQ=255) gCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCGGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTtagccta < 1:365940/150‑1 (MQ=255) tGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGa > 2:278679/1‑91 (MQ=255) tGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGa < 1:278679/91‑1 (MQ=255) tGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTg < 2:393507/129‑1 (MQ=255) tGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTg > 1:393507/1‑129 (MQ=255) gCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGATGTAGGAGCGGGTTGACCCGCGAAGAGGGCGGTAAAGGCGAAATGAGGAGCCGAAAAGGCAGCGTCGATCTGTCGCGCCGCGATCTGGAACGCGCGTGTtcgactagcag > 2:202000/1‑148 (MQ=37) tctTCGCGGGTAAACCCGCTGCTACGA‑G‑GGACCGCGATGAAAGAGCGGGGTTACCCGCGATAGGGCCGGTACAGGCGAAATTCTGAACCGAAAAACCAGAGTCCATCTTTCGCGCCCCGCGCTGGAGCGCTCGTGTTAGCTTAGACGACt > 1:256629/1‑150 (MQ=255) tctTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCCCGTGTTAGCCTAGCCGGCt > 2:116671/1‑150 (MQ=255) aCCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTcg < 2:451280/99‑1 (MQ=255) aCCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTcg > 1:451280/1‑99 (MQ=255) cccGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACaagaa > 1:364089/1‑150 (MQ=255) cccGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACaagaa > 1:585778/1‑150 (MQ=255) cGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAagaaga < 2:364089/150‑1 (MQ=255) cGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGcaa > 2:654949/1‑60 (MQ=17) cGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGGcaa < 1:654949/62‑3 (MQ=17) tGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCATAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAAcc < 2:289590/150‑1 (MQ=255) gCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACg > 1:515601/1‑137 (MQ=255) gCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACg < 2:515601/137‑1 (MQ=255) gCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCa > 1:113292/1‑150 (MQ=255) ccGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGa < 2:187611/125‑1 (MQ=255) ccGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGa > 1:187611/1‑125 (MQ=255) cgcgAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCATAGCGACAGAt > 2:532696/1‑150 (MQ=255) cGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCATAGCGACAGATGAACCGCAc > 2:498499/1‑148 (MQ=255) cGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCATAGCGACAGATGAACCGCAc < 1:498499/148‑1 (MQ=255) | GTGGATAGTTTGGTGGTGAGGCGCCGCCGGGTCTTGGCCAGCGCTTGAGCCTTGTGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACGA‑G‑GGACCGCGTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGTACAGGCGAAATGCTGAGCCGAAAATGCAGAGTCGATCTGTCGCGCCCCGATCTGGAACGCGCGTGTTAGCCTAGCCGGCTACCTCTGACAAGAAGACTGCAGCCTATGACGCAAACCTCACCCATAGCGACAGATGAACCGCAC > NC_002947/5506542‑5506826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |