Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,507,701 C→A 12.1% S305* (TCG→TAG)  cycA → D‑alanine/beta‑alanine/D‑serine/glycine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,507,7010CA12.1% 90.4 / 3.3 33S305* (TCG→TAG) cycAD‑alanine/beta‑alanine/D‑serine/glycine permease
Reads supporting (aligned to +/- strand):  ref base C (17/12);  new base A (2/2);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TGATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCACCTGCCTGTTGCTCGGTGCGG  >  NC_002947/5507563‑5507850
                                                                                                                                          |                                                                                                                                                     
tGATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcg                                                                                                                                            >  2:424618/1‑150 (MQ=255)
tGATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcg                                                                                                                                            <  2:694949/150‑1 (MQ=255)
       gATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGtt                                                                                                                                     >  1:102088/1‑150 (MQ=255)
           aTGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTcc                                                                                                                                 <  2:607724/150‑1 (MQ=255)
                       ccGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATg                                                                                                                     >  1:182935/1‑150 (MQ=255)
                             cgcgATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTAc                                                                                                               <  1:210784/150‑1 (MQ=255)
                              gcgATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACg                                                                                                              >  2:635156/1‑150 (MQ=255)
                                      gtgCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCg                                                                                                      >  2:532579/1‑150 (MQ=255)
                                                            ttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcggc                                                                                                                                          <  2:599549/92‑1 (MQ=255)
                                                            ttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcggc                                                                                                                                          >  1:599549/1‑92 (MQ=255)
                                                            ttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAgg                                                                                >  1:192250/1‑150 (MQ=255)
                                                            ttGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAgg                                                                                >  1:530097/1‑150 (MQ=255)
                                                                gttgttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGTTTCGTCGGCCAACAGCGGCGTGTTCTTCACCAGCAGCATTCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCttt                                                                            >  2:35202/1‑150 (MQ=255)
                                                                 ttgttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTc                                                                           >  2:140685/1‑150 (MQ=255)
                                                                 ttgttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTc                                                                           >  2:320297/1‑150 (MQ=255)
                                                                   gttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCga                                                                         <  1:138873/150‑1 (MQ=255)
                                                                    ttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcggc                                                                                                                                          <  2:681348/84‑1 (MQ=255)
                                                                    ttCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAgcggc                                                                                                                                          >  1:681348/1‑84 (MQ=255)
                                                                       gTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGACAAg                                                                     <  1:424618/150‑1 (MQ=255)
                                                                             gCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCg                                                               >  1:237818/1‑150 (MQ=255)
                                                                               atgCTTGCCGCCGCCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAg                                                             >  2:297187/3‑150 (MQ=255)
                                                                                         gcggcggcAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTg                                                   <  1:320297/150‑1 (MQ=255)
                                                                                              ggcAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGc                                              >  2:77868/1‑150 (MQ=255)
                                                                                                 aaGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGcc                                                           >  2:414215/1‑134 (MQ=255)
                                                                                                 aaGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGcc                                                           <  1:414215/134‑1 (MQ=255)
                                                                                                           aaCTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTAt                                 >  1:702107/1‑150 (MQ=255)
                                                                                                                            cTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCAcctgcct                <  2:192250/150‑1 (MQ=255)
                                                                                                                            cTCGGCGGCTTCGTAGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGGGCCGTTCGGTGCCGGCCAACGGCCTGTATTtctc                            <  1:663841/138‑1 (MQ=255)
                                                                                                                            cTCGGCGGCTTCGTAGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGGGCCGTTCGGTGCCGGCCAACGGCCTGTATTtctc                            >  2:663841/1‑138 (MQ=255)
                                                                                                                            cTCGGCGGCTTCGTAGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTtctc                            >  2:663577/1‑138 (MQ=255)
                                                                                                                            cTCGGCGGCTTCGTAGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTtctc                            <  1:663577/138‑1 (MQ=255)
                                                                                                                                  ggcTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCACCTGCCTGTTGCt          <  1:532579/150‑1 (MQ=255)
                                                                                                                                   gcTTCGTCGGGCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGACACTGGGTAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCACCTGCCTGTTGCTc         <  2:102088/150‑1 (MQ=255)
                                                                                                                                          cgGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCACCTGCCTGTTGCTcggtgcgg  <  1:685834/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                     
TGATCGCGATCATGGCCGTGACCCCGTGGCGCGATGTGGTGCCCGGAAAAAGCCCGTTCGTTGAGTTGTTCGTGCTGGCAGGCTTGCCGGCGGCGGCAAGCATCATCAACTTCGTGGTGCTGACCTCGGCGGCTTCGTCGGCCAACAGCGGCGTGTTCTCCACCAGCCGCATGCTCTACGGCCTGTCGCAGGAAGGTGACGCGCCCAAGGCTTTCGAGAAGCTGTCGAGCCGTTCGGTGCCGGCCAACGGCCTGTATTTCTCGTGCACCTGCCTGTTGCTCGGTGCGG  >  NC_002947/5507563‑5507850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑