| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,737,658 | G→C | 12.5% | intergenic (‑157/+45) | hutU ← / ← PP_5034 | urocanate hydratase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,737,658 | 0 | G | C | 12.5% | 77.5 / 5.6 | 32 | intergenic (‑157/+45) | hutU/PP_5034 | urocanate hydratase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/13); new base C (2/2); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
TGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAAC > NC_002947/5737515‑5737793 | tGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGgcggcg < 1:684590/150‑1 (MQ=255) gcgggTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTGGAGATTTATGTTGGGGTATATATCGAGCGCCGCGCGGGCGGCGCGCGATCTCACAGgc > 2:720727/1‑150 (MQ=255) tGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTa < 1:348532/150‑1 (MQ=255) aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgc > 1:132095/1‑133 (MQ=255) aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgc < 2:132095/133‑1 (MQ=255) aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGcc > 1:678366/1‑150 (MQ=255) aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGGCGCTCGa > 2:602139/1‑123 (MQ=255) aaTATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCCCGCGCGGCGCTCGa < 1:602139/123‑1 (MQ=255) aaaCACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcgc > 1:586835/1‑128 (MQ=255) aaaCACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGgcgc < 2:586835/128‑1 (MQ=255) aaaCACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCtt > 2:235020/1‑150 (MQ=255) cTTTACTTGTATGTCCGAGCTTGGGGTGCCGTTATAAGCTGACCTTACCGTCCCTGGATTGTGCTTTGCAAGGTAGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGccc < 1:203321/150‑1 (MQ=16) cTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGccc > 2:641831/1‑150 (MQ=255) tatgtaCAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCACCCATCAAGTTc > 2:73594/1‑150 (MQ=255) gtaCAAGCATGGACAACCAAAATAATCGTACCTTACCGTTCCTGGCTTGAGATTTATGTCGTGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGAt < 1:464113/150‑1 (MQ=255) aaGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAATTTCGATCAgg < 1:641831/150‑1 (MQ=255) aaGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAgg < 2:678366/150‑1 (MQ=255) aaGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAgg > 1:136084/1‑150 (MQ=255) aaGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAgg > 1:17626/1‑150 (MQ=255) aCCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGtt < 1:648750/131‑1 (MQ=255) aCCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGtt > 2:648750/1‑131 (MQ=255) aCCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGccc < 2:17626/150‑1 (MQ=255) cAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCt > 1:56685/1‑150 (MQ=255) aatCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACaa < 2:136084/150‑1 (MQ=255) aCCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCTAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGcc > 2:261110/1‑150 (MQ=255) cTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCAtt < 1:235020/150‑1 (MQ=255) tgtgaGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTc > 2:333029/5‑138 (MQ=255) tgtgaGATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTc < 1:333029/134‑1 (MQ=255) ataGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGt < 1:720727/150‑1 (MQ=255) gATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGa > 1:591763/1‑150 (MQ=255) aTCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGaa > 2:504733/1‑150 (MQ=255) ccGAGCGCCGCGCGGGCGGCGCTCGATCTCACACGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCCCCCCTGCACAACCAGCCCCAGCAACTCCTCATTGCCTTCCAGCCGCAAGCAGTCGaac > 2:353263/2‑150 (MQ=255) | TGCGGTCGTCCGCGGGCGGGTATGTCGTGGTCAATATACAAACACACCTTTACTTGTATGTACAAGCATGGACAACCAAAATAATCGGACCTTACCGTTCCTGGCTTGAGATTTATGTTGGGGTATAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCCAAAAATCTACAGCCGAACCTTCAGCGCCCATCAAGTTCGATCAGGCAGCACCGCCCCTGCACAACCAGCCCCAGCAATTCATCATTGCCTTCCAGCCGCAAGCAGTCGAAC > NC_002947/5737515‑5737793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |