| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,849,104 | A→G | 12.4% | intergenic (+23/‑248) | rlmI → / → ilvD | ribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,849,104 | 0 | A | G | 12.4% | 120.5 / 8.1 | 49 | intergenic (+23/‑248) | rlmI/ilvD | ribosomal RNA large subunit methyltransferase I/dihydroxy‑acid dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (18/24); new base G (3/3); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01 | |||||||||||
TGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTC > NC_002947/5848960‑5849227 | tGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGc > 2:594858/1‑150 (MQ=255) gCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTc < 1:702515/150‑1 (MQ=255) gctgctCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAAt > 2:725262/1‑150 (MQ=255) gctgctCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTCCCACACAGCTGATATCTGCCAAAAACCGCCGCCCCGCGGCCCTTCCCCGCCGCaac > 2:94088/1‑149 (MQ=255) tgctCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATcc < 2:743418/150‑1 (MQ=255) agcgagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGc > 1:294554/1‑150 (MQ=255) gcgagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGGCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGGCTCAATCCCCCCGcc > 1:717929/1‑150 (MQ=255) gcgagGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGcc > 2:394146/1‑150 (MQ=255) ggCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATc > 2:201669/1‑150 (MQ=255) cACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTccc < 1:541158/150‑1 (MQ=255) aCCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCt > 2:75807/1‑150 (MQ=255) ccGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCa > 1:745715/1‑150 (MQ=255) ggTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCAcc > 2:369481/1‑150 (MQ=255) ccGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCtttt > 1:282888/1‑96 (MQ=255) ccGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCtttt < 2:282888/97‑2 (MQ=255) cGGTCATCGCCGAAACCCGCTAAATCAAAGGCACCCCCAGCCGGTTACTACCAAACCGCTAATATCTGCCAAAAAGGGCCGTCCAACGGCCTTTCCCCGCCGCAATCCCCCCGCCAACCATCCACATCTGCATCCCCTCACCGCGCCAGc < 2:722153/150‑1 (MQ=255) ggCCATCGCCTAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACTGCTGCTATCTTCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAATCATCCACATCTGCATTCCCTCACCGCGCCAGCg < 1:127701/150‑1 (MQ=255) ggCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCg < 1:185813/150‑1 (MQ=255) gCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATc > 1:307913/1‑120 (MQ=255) gCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATc < 2:307913/120‑1 (MQ=255) cATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACGGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCttttt > 1:511298/1‑91 (MQ=38) cATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACGGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCttttt < 2:511298/93‑3 (MQ=38) ccGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGaa > 2:100852/1‑150 (MQ=255) cGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAAt < 1:394146/150‑1 (MQ=255) aCCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGc < 2:294554/150‑1 (MQ=255) ccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCg < 1:441069/135‑1 (MQ=255) ccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCg > 2:441069/1‑135 (MQ=255) ccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCt > 1:34098/1‑150 (MQ=255) ccGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCt > 1:256712/1‑150 (MQ=255) aTCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATc < 1:725262/150‑1 (MQ=255) aTCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATc < 2:745715/150‑1 (MQ=255) aaaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCCCTGCTCTATTTCCTCCCCCGGTGAGTGGTGTAGAATCGGCCTATTCATCGCCa < 1:94088/150‑1 (MQ=255) aaaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCa < 2:256712/150‑1 (MQ=255) aaaGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCa < 1:449708/150‑1 (MQ=255) gCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTTCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTc < 1:594858/150‑1 (MQ=255) tCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATc < 2:451154/150‑1 (MQ=255) ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGc > 1:109471/1‑63 (MQ=255) ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGc < 2:109471/63‑1 (MQ=255) ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATc > 1:305217/1‑143 (MQ=255) ccGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATc < 2:305217/143‑1 (MQ=255) cGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggcgg < 1:201669/150‑1 (MQ=255) gTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCtttt < 2:136108/53‑2 (MQ=21) gTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCTGGGCGGCCCtttt > 1:136108/1‑52 (MQ=21) cTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTAt < 2:332250/150‑1 (MQ=255) gCCAAACAGCTGATATCTGCCAAAAAGCGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGa < 2:336193/150‑1 (MQ=255) cAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGc > 2:41229/1‑150 (MQ=255) cTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTc < 2:34098/150‑1 (MQ=255) tGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCTcggc < 2:682215/130‑1 (MQ=255) tGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCcggc > 1:682215/1‑130 (MQ=255) | TGGACCGCAACATGCAGCTGCTCGAGCGAGGCGGTCAGGGCCCGGATCACCCGGTACACCCGGCCATCGCCGAAACCCGCTACATCAAAAGCATCACCTGCCGGTTACTGCCAAACAGCTGATATCTGCCAAAAAGGGCCGCCCAGCGGCCCTTTCCTGCCGCAATCCCCCCGCCAACCATCCACATCTGCATTCCCTCACCGCGCCAGCGGTGTAGAATCGGCCTATTCATCGCCAGTCATCCCCGGCGGGTTTATGAGCTCTGGTC > NC_002947/5848960‑5849227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |