Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,920,560 A→G 8.2% N364D (AAC→GAC)  PP_5190 → type II secretion system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,920,5600AG 8.2% 136.8 / 2.9 49N364D (AAC→GAC) PP_5190type II secretion system protein
Reads supporting (aligned to +/- strand):  ref base A (27/18);  new base G (2/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01

GCCTTGGCCTGCACGGCCAGCAACTGGCCCAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCGAAATCCGC  >  NC_002947/5920414‑5920709
                                                                                                                                                  |                                                                                                                                                     
gCCTTGGCCTGCACGGCCAGCAACTGGCCCAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAAcc                                                                                                                                                    >  2:704368/1‑150 (MQ=255)
       ccTGCACGGCCAGCAACTGGCCCAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCAc                                                                                                                                             >  1:352111/1‑150 (MQ=255)
                           cccAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAgc                                                                                                                         >  1:618767/1‑150 (MQ=255)
                            acAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAgcg                                                                                                                        >  1:448891/2‑150 (MQ=255)
                                  gCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCAt                                                                                                                                          <  1:742298/126‑1 (MQ=255)
                                  gCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCAt                                                                                                                                          >  2:742298/1‑126 (MQ=255)
                                  gCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGa                                                                                                                  >  2:67944/1‑150 (MQ=255)
                                  gCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGa                                                                                                                  <  1:559515/150‑1 (MQ=255)
                                  gCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGa                                                                                                                  <  1:553287/150‑1 (MQ=255)
                                     gcgcCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGagcc                                                                                                               >  1:711229/1‑150 (MQ=255)
                                           gCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCtt                                                                                                         <  1:704368/150‑1 (MQ=255)
                                                   aGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCtgt                                                                                                                                                 >  1:451365/1‑102 (MQ=255)
                                                   aGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCtgt                                                                                                                                                 <  2:451365/102‑1 (MQ=255)
                                                        cAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAg                                                                                            >  2:233830/1‑150 (MQ=255)
                                                               tcatcCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAAc                                                                                                                                                     <  1:496259/86‑1 (MQ=255)
                                                               tcatcCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAAc                                                                                                                                                     >  2:496259/1‑86 (MQ=255)
                                                                     tGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCgcgc                                                                               >  2:111068/1‑150 (MQ=255)
                                                                           ccGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGaa                                                                                                                                      <  1:546204/89‑1 (MQ=255)
                                                                           ccGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGaa                                                                                                                                      >  2:546204/1‑89 (MQ=255)
                                                                            cGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCg                                                                                                                                        <  1:739585/86‑1 (MQ=255)
                                                                            cGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCg                                                                                                                                        >  2:739585/1‑86 (MQ=255)
                                                                               ccccACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTggg                                                                     <  1:233830/150‑1 (MQ=255)
                                                                                         tCCGGCAAGACCACCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCATCCCGCGCTGGACCTGGGGTTTGCCAAc                                                           <  1:320031/150‑1 (MQ=255)
                                                                                                  aCCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAgg                                                  >  2:2078/1‑150 (MQ=255)
                                                                                                  aCCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAgg                                                  <  1:476226/150‑1 (MQ=255)
                                                                                                  aCCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACACGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGTAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAgg                                                  <  2:541289/150‑1 (MQ=255)
                                                                                                   ccAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAggg                                                 >  2:271584/1‑150 (MQ=255)
                                                                                                    cAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGc                                                >  2:523664/1‑150 (MQ=255)
                                                                                                     aGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGcc                                               >  2:470619/1‑150 (MQ=255)
                                                                                                      gCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTTGACCtgtg                                                                                                                                                <  1:559929/52‑1 (MQ=255)
                                                                                                      gCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTTGACCtgtg                                                                                                                                                >  2:559929/1‑52 (MQ=255)
                                                                                                             gTACACCAGCCTCAAACTGCTGGCCACACCACAGGTTGACCtgtggt                                                                                                                                              >  1:262625/1‑45 (MQ=255)
                                                                                                             gTACACCAGCCTCAAACTGCTGGCCACACCACAGGTTGACCtgtggt                                                                                                                                              <  2:262625/47‑3 (MQ=25)
                                                                                                              tACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTgcgc                                      <  2:618767/150‑1 (MQ=255)
                                                                                                                 acCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCaa                                   <  1:271584/150‑1 (MQ=255)
                                                                                                                   cAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGa                                 >  2:420054/1‑150 (MQ=255)
                                                                                                                     gCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGAcc                               <  2:380565/150‑1 (MQ=255)
                                                                                                                          aaaCTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGAc                          >  1:502467/1‑150 (MQ=255)
                                                                                                                                   gCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATg                 >  2:714156/1‑150 (MQ=255)
                                                                                                                                     cacaccacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGAt               >  1:744779/1‑150 (MQ=255)
                                                                                                                                       caccacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCg             <  1:67944/150‑1 (MQ=255)
                                                                                                                                        accacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCgg            <  1:420054/150‑1 (MQ=255)
                                                                                                                                          cacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCg          >  2:537797/1‑150 (MQ=255)
                                                                                                                                          cacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCg          >  2:236656/1‑150 (MQ=255)
                                                                                                                                          cacaGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCCGTCAGGCCCATGCTGCGCCACGACCCCGCCATCACCATGATCGGCg          >  2:50642/1‑150 (MQ=255)
                                                                                                                                               gTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAAc                                                           <  2:168448/96‑1 (MQ=255)
                                                                                                                                               gTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAAc                                                           >  1:168448/1‑96 (MQ=255)
                                                                                                                                               gTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCGAAATc     >  1:66183/1‑150 (MQ=255)
                                                                                                                                                 cAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTACGCCAAGACCCCGACATCATCATGATCGGCGAAATccg   >  2:595217/1‑150 (MQ=255)
                                                                                                                                                  aaCCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCGAAATccgc  <  2:744779/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCCTTGGCCTGCACGGCCAGCAACTGGCCCAATGGCAGCGCCTGCTTCGTCAGCGCCAAGGCATCATCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCAGCACGCTGTACACCAGCCTCAAACTGCTGGCCACACCACAGGTCAACCTGTGCACCATCGAAGACCCGATCGAGCGCCTGGAGCCAGCCTTCAACCAGTTGCAGGTGCAGCCCGCGCTGGACCTGGGGTTTGCCAACGCGGTCAGGGCCATGCTGCGCCAAGACCCCGACATCATCATGATCGGCGAAATCCGC  >  NC_002947/5920414‑5920709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑