| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 247,399 | G→C | 9.5% | S526T (AGT→ACT) | yheS → | ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 247,399 | 0 | G | C | 9.5% | 123.1 / 5.8 | 42 | S526T (AGT→ACT) | yheS | ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (21/17); new base C (2/2); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01 | |||||||||||
TGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCT > NC_002947/247256‑247528 | tGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGcc < 2:233486/150‑1 (MQ=255) aGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGc > 1:561633/1‑150 (MQ=255) tgtCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCAc > 2:174971/1‑150 (MQ=255) gtCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCAcc > 2:678881/1‑150 (MQ=255) gCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGAc < 1:678881/150‑1 (MQ=255) cAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGa < 1:644487/150‑1 (MQ=255) accacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGaaa < 2:561633/150‑1 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTa > 1:262165/1‑113 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTa < 2:262165/113‑1 (MQ=255) cacTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGc > 2:714228/1‑150 (MQ=255) ggCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGc < 1:82698/150‑1 (MQ=255) ggCCGACGGCAAGGTCGACACATTCGACGGGGGCCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGc < 2:470674/150‑1 (MQ=255) cGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGCCTGAAGCGAACGGGTCAGTCG‑GTATGTCAAGGTCGGTGGGCCGGCCAGTGCTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCtt > 1:655955/1‑150 (MQ=255) cGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc < 2:247758/99‑1 (MQ=255) cGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGc > 1:247758/1‑99 (MQ=255) cGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCtt > 1:461486/1‑150 (MQ=255) gTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTcagcag > 2:335567/1‑150 (MQ=255) gccacCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGa < 2:348769/93‑1 (MQ=255) gACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTACGCCGGCCAGTACTGCACCGGCCAACCCGGa > 1:348769/1‑95 (MQ=255) acacCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCGGCGCACTgcgc > 2:337108/1‑66 (MQ=255) acacCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCGGCGCACTgcgc < 1:337108/66‑1 (MQ=255) cTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCAcc > 2:82999/1‑150 (MQ=255) cTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGAACCGTTCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCAcc > 1:49799/1‑150 (MQ=255) ttCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGa < 1:578840/94‑1 (MQ=255) ttCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGa > 2:578840/1‑94 (MQ=255) tGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACaagaa > 1:92820/1‑150 (MQ=255) tGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACaagaa < 1:383084/150‑1 (MQ=255) tGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACaagaa < 1:335567/150‑1 (MQ=255) aCCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTg < 2:34894/134‑1 (MQ=255) aCCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTg > 1:34894/1‑134 (MQ=255) cTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCt > 2:349427/1‑150 (MQ=255) tACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGCCAAg > 2:543388/1‑150 (MQ=255) tACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAg > 1:599253/1‑150 (MQ=255) tACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAg > 1:17352/1‑150 (MQ=255) aGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTg < 1:174971/150‑1 (MQ=255) gC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGCACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTgg > 2:545149/1‑150 (MQ=255) gctggTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGTCTGCCGACAAGCTGGAAACCGa < 1:714228/150‑1 (MQ=255) tggTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTg < 2:220203/110‑1 (MQ=255) tggTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTg > 1:220203/1‑110 (MQ=255) ggTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCt < 2:17352/150‑1 (MQ=255) tggccggcgcACTGCGCTGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGc > 2:157384/7‑140 (MQ=255) tggccggcgcACTGCGCTGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGc < 1:157384/134‑1 (MQ=255) | TGCAGTGGTGGTGGTGTCCCACGACCGTCATTTGCTCAAGAGCACCACTGATGACTTCCTGCTGGTGGCCGACGGCAAGGTCGACACCTTCGACGGTGACCTGGAGGACTACAGC‑CGCTGGCTGGTCGAGTACCGCCAGCGCAGTGCGCCGGCCAGTACTGCACCGGCCAACCCGGACAAGACCGACAAGAAAGCCCAGCGCCAGGCTGCCGCAGCCTTGCGTCAGCAGTTGGCACCGCACAAGAAGGCTGCCGACAAGCTGGAAACCGAGCT > NC_002947/247256‑247528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |