Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 274,921 G→T 6.9% Q343K (CAG→AAG)  PP_0222 ← DszA family monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947274,9210GT 6.9% 188.0 / 2.6 58Q343K (CAG→AAG) PP_0222DszA family monooxygenase
Reads supporting (aligned to +/- strand):  ref base G (25/29);  new base T (2/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

TTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGTTTGG  >  NC_002947/274776‑275067
                                                                                                                                                 |                                                                                                                                                  
ttGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGgcgc                                                                                                                                                >  2:73407/1‑150 (MQ=255)
ttGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGgcgc                                                                                                                                                >  1:319519/1‑150 (MQ=255)
    aaCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTgg                                                                                                                                            <  1:260780/150‑1 (MQ=255)
    aaCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTgg                                                                                                                                            >  2:260780/1‑150 (MQ=255)
     aCCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGc                                                                                                                                           >  1:148509/1‑150 (MQ=255)
        gTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGa                                                                                                                                        >  1:616351/1‑150 (MQ=255)
            aGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTgg                                                                                                                                    >  1:184163/1‑150 (MQ=255)
              gCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTTGCGCGTGGCTGACTGGAt                                                                                                                                  >  2:493484/1‑150 (MQ=255)
              gCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGAt                                                                                                                                  <  2:30701/150‑1 (MQ=255)
               ccGGTTTCATCGATCCACGCGATCAACTGCCCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGCTTGCCTGAATg                                                                                                                                 <  2:24100/150‑1 (MQ=255)
                 ggTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGc                                                                                                                               <  2:616351/150‑1 (MQ=255)
                           aTCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTgg                                                                                                                     <  2:618951/150‑1 (MQ=255)
                                cgcgATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGaa                                                                                                                <  2:616854/150‑1 (MQ=255)
                                       aaCTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATg                                                                                                         <  1:329911/150‑1 (MQ=255)
                                         cTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTTAAGGCAATggg                                                                                                       <  2:184163/150‑1 (MQ=255)
                                         cTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATggg                                                                                                       <  2:478558/150‑1 (MQ=255)
                                                 ccACCTTTTCAGGCGCGCCGACCAGGTTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCa                                                                                               >  2:578021/1‑150 (MQ=255)
                                                   aCCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTc                                                                                                 >  1:588469/1‑146 (MQ=255)
                                                   aCCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTc                                                                                                 <  2:588469/146‑1 (MQ=255)
                                                   aCCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGt                                                                                             <  1:73407/150‑1 (MQ=255)
                                                     cTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTc                                                                                           <  2:148509/150‑1 (MQ=255)
                                                     cTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGAGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTc                                                                                           >  2:499636/1‑150 (MQ=255)
                                                             gcgcgcCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAggcgg                                                                                   >  1:254699/1‑150 (MQ=255)
                                                             gcgcgcCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAggcgg                                                                                   >  1:344291/1‑150 (MQ=255)
                                                              cgcgcCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAggcggc                                                                                  <  2:319519/150‑1 (MQ=255)
                                                                 gcCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGaa                                                                               >  2:386334/1‑150 (MQ=255)
                                                                 gcCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGaa                                                                               >  2:48122/1‑150 (MQ=255)
                                                                   cGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGt                                                                             <  1:598788/150‑1 (MQ=255)
                                                                       cAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGAt                                                                         <  1:386334/150‑1 (MQ=255)
                                                                                gTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGa                                                                >  2:487350/1‑150 (MQ=255)
                                                                                         gccCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGaa                                                       <  1:487350/150‑1 (MQ=255)
                                                                                         gccCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGaa                                                       <  1:742134/150‑1 (MQ=255)
                                                                                           ccAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGt                                                     >  1:744800/1‑150 (MQ=255)
                                                                                             aaGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAACTgc                                                   <  2:150725/150‑1 (MQ=255)
                                                                                                   tGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTCGCCGACTGGAATCTATGCCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGAcc                                             <  1:578021/150‑1 (MQ=255)
                                                                                                       gTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGtt                                                                                                     <  2:315976/90‑1 (MQ=255)
                                                                                                       gTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGtt                                                                                                     >  1:315976/1‑90 (MQ=255)
                                                                                                           cAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTc                                     >  2:722065/1‑150 (MQ=255)
                                                                                                               aGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGAGGCGAAGTGCGCGACCCCCGCTTCGgcg                                 <  2:344461/150‑1 (MQ=255)
                                                                                                                ggCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTTGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGgcgc                                >  1:181544/1‑150 (MQ=255)
                                                                                                                  cgcCGGCGGGTCCAGGCGTTGTCTTGCAACTTGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCtg                              <  2:181544/150‑1 (MQ=255)
                                                                                                                  cgcCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCtg                              <  2:254699/150‑1 (MQ=255)
                                                                                                                           gTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCa                                                                                          <  1:496522/81‑1 (MQ=255)
                                                                                                                           gTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCa                                                                                          >  2:496522/1‑81 (MQ=255)
                                                                                                                            tCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCa                    >  2:82345/1‑150 (MQ=255)
                                                                                                                            tCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTC‑‑AGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAgg                  >  1:237184/1‑150 (MQ=255)
                                                                                                                                ggCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCa                                                                                          >  1:506313/1‑76 (MQ=255)
                                                                                                                                ggCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCa                                                                                          <  2:506313/76‑1 (MQ=255)
                                                                                                                                   gTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTc             >  1:456104/1‑150 (MQ=255)
                                                                                                                                   gTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTc             <  2:456104/150‑1 (MQ=255)
                                                                                                                                    ttGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCg            >  1:319037/1‑150 (MQ=255)
                                                                                                                                    ttGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTC‑‑AGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGc          <  2:237184/150‑1 (MQ=255)
                                                                                                                                     tGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCgg           <  1:308927/150‑1 (MQ=255)
                                                                                                                                        cTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCAt        <  1:722065/150‑1 (MQ=255)
                                                                                                                                           gCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGtt     >  2:703407/1‑150 (MQ=255)
                                                                                                                                            cAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGttt    >  2:709079/1‑150 (MQ=255)
                                                                                                                                             aaCTTGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGTTTg   <  1:493484/150‑1 (MQ=255)
                                                                                                                                              aCTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGTTTgg  <  1:48122/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
TTGAAACCGTCCAGGCCGGTTTCATCGATCCACGCGATCAACTGCTCGGCCACCTGTTCAGGCGCGCCGACCAGGGTTACGTAGCGGCCGCCCAAGGCGTGCTGTTGCAACAGGCGCCGGCGGGTCCAGGCGTTGTCTTGCAACTGGCGCGTGGCTGACTGGATGGCGTTGCCCTGGTTGAAGGCAATGGGTTGGTCCAGTTCATAGGCGGCGAAGTCGATGCCAGTGGACGCGGCGAAGTGCGCGACCCCCGCTTCGGCGCTGGCGTGGCGCAGGTACTCGGCATGTTTGG  >  NC_002947/274776‑275067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑