| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,041,669 | T→C | 11.4% | D104G (GAC→GGC) | rph ← | ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,041,669 | 0 | T | C | 11.4% | 89.7 / 3.3 | 35 | D104G (GAC→GGC) | rph | ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (21/10); new base C (2/2); total (23/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01 | |||||||||||
GCCGCCGCGCTTCTTGATCACTGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCTTGGCCCTG > NC_002947/6041530‑6041817 | gccgccGCGCTTCTTGATCACTGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAgcgcggc < 1:286041/150‑1 (MQ=255) cgcgCTTCTTGATCACTGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCa > 2:470728/1‑150 (MQ=255) tCACTGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACTGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGa > 1:697135/1‑150 (MQ=255) tGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAg > 1:588002/1‑150 (MQ=255) tGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCAc < 2:615097/150‑1 (MQ=255) ggTGATGGACCCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACTGATAAACATTACTCAGCTCGATCATGTCCAGCGAGGCGCGCAGCGACCGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCtt < 1:242751/150‑1 (MQ=255) gTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTg > 2:246956/1‑150 (MQ=255) aTGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTACTTGCCa > 2:288369/1‑150 (MQ=255) aCGCGGTACGGGTACCGCCGGTGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGAAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACggc < 1:228398/150‑1 (MQ=255) gggTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTcgc > 1:319051/1‑150 (MQ=255) gTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCgcgc > 1:679543/1‑150 (MQ=255) ccGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACTGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTgg < 2:697135/150‑1 (MQ=255) gCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGtt > 1:322892/1‑150 (MQ=255) cATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTAc > 2:250223/1‑150 (MQ=255) cTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGcc > 1:54357/1‑150 (MQ=255) gATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCt < 2:634320/134‑1 (MQ=255) gATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCt > 1:634320/1‑134 (MQ=255) aTCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTg > 2:691235/1‑150 (MQ=255) tCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCa > 2:148996/1‑93 (MQ=255) tCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCa < 1:148996/93‑1 (MQ=255) cGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGgcagca > 2:725419/1‑150 (MQ=255) gTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGtt < 1:515769/120‑1 (MQ=255) gTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGtt > 2:515769/1‑120 (MQ=255) tACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCa > 1:548198/1‑150 (MQ=255) ggTGATGTCACCGAGCTTGCTCATGCCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCtt > 2:743257/1‑90 (MQ=255) ggTGATGTCACCGAGCTTGCTCATGCCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCtt < 1:743257/90‑1 (MQ=255) tGTCACCGAGCTTGCTCATGCCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAg > 2:690736/1‑67 (MQ=255) tGTCACCGAGCTTGCTCATGCCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAg < 1:690736/67‑1 (MQ=255) aCCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTcg < 2:451564/142‑1 (MQ=255) aCCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTcg > 1:451564/1‑142 (MQ=255) aCCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTcag > 2:676637/1‑150 (MQ=255) ttGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGAGCTGGATATCCAGCGGGCGGCCACCCTGCTTGCTACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCt > 1:655938/1‑150 (MQ=255) tCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCTTGGc > 1:431413/1‑150 (MQ=255) gTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCTTGGCCCTg < 2:54357/150‑1 (MQ=255) gTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCTTGGCCCTg > 1:466080/1‑150 (MQ=255) | GCCGCCGCGCTTCTTGATCACTGCCAAAGCGTCGCACAAGGCAACCATGGCGCCGGTGATGGACGCGGTACGGGTACCGCCATCGGCCTGGATCACGTCGCAATCGACGTACAGGGTGATGTCACCGAGCTTGCTCATGTCCAGCGCGGCGCGCAGCGAACGGCCGATCAGGCGCTGGATTTCCAGGGTGCGGCCACCCTGCTTGCCACGGCTGGCTTCGCGCTGGTTACGTTCGCCGGTGGAGCGCGGCAGCATGCCATACTCGGCGGTCAGCCAGCCTTGGCCCTG > NC_002947/6041530‑6041817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |