| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 321,742 | T→C | 11.1% | intergenic (+27/+431) | aguA → / ← PP_0267 | agmatine deiminase/ferric siderophore receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 321,742 | 0 | T | C | 11.1% | 105.8 / 5.4 | 45 | intergenic (+27/+431) | aguA/PP_0267 | agmatine deiminase/ferric siderophore receptor |
| Reads supporting (aligned to +/- strand): ref base T (21/19); new base C (3/2); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
GCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGG > NC_002947/321602‑321887 | gCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGc < 2:427636/150‑1 (MQ=255)ccctggATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCAGGCGCCGGTCAAGCGCTGAGCCAGTAATGAATGCGCGGCCCGTCATGTGACGGGc > 1:26054/5‑150 (MQ=255) aaGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCct > 1:732509/1‑150 (MQ=255) ccGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCttt > 2:424684/1‑143 (MQ=255) ccGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCttt < 1:424684/143‑1 (MQ=255) cGACCATGAGTTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCCAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGcgcg > 1:199509/1‑150 (MQ=255) tGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGAc > 1:110907/1‑150 (MQ=255) gtggtgATGATCCCGGGCCGCAAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCAGTAATGAATGAGAGGCCCGTCATGTGACGGGCATCTTTTTTGCGCGGGCGACGcac > 2:176665/1‑148 (MQ=255) gtgATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGACCGTAACATATGTGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCAGGCGGCGCGCGGa > 2:551864/1‑150 (MQ=255) gtgATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACACAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGa > 2:666105/1‑150 (MQ=255) tgatgaTCCCGGGCCGCGTGTATCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCACCCGGCGCCGGTCAAGCGCTGAGCCCGTAATTAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGaa < 1:413971/150‑1 (MQ=255) gatCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCttt > 1:322097/1‑124 (MQ=255) gatCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCttt < 2:322097/124‑1 (MQ=255) gatCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCg > 2:438975/1‑150 (MQ=255) gatCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCACATGACgg < 2:408112/116‑1 (MQ=255) gatCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCACATGACgg > 1:408112/1‑116 (MQ=255) tCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAg > 1:89806/1‑150 (MQ=255) cccGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGc < 2:89806/150‑1 (MQ=255) cGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTg > 1:104034/1‑150 (MQ=255) gggCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTgg < 1:454845/150‑1 (MQ=255) gAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGt > 2:37688/1‑150 (MQ=255) gctCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGt > 1:457984/1‑150 (MQ=255) ctctTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGAGACGGTCTAGTGCTGATCCCGTAAAAAAAGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTa < 1:355934/150‑1 (MQ=255) tGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTC‑TTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGtt < 2:385237/150‑1 (MQ=255) cAACATTCACTTCCTGACCCAACCGCATCCGGCGCCGGTCAATCCCTTAGCCCGTAATGAATTAGAGGCCCGTCATGTTACGGGCCTCTTTTTTACGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCa < 1:176665/150‑1 (MQ=255) cAACATTCACTGCCTGACCCAACAGAAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAAAAAACGAGAGACCAGTCATGTGAAGGCACACTTTTTTGCGTGGTCGACGCCTTGTAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCa < 2:273966/150‑1 (MQ=255) aCTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCaa > 1:326782/1‑150 (MQ=255) ccTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATg > 2:675512/1‑150 (MQ=255) ccAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGAACTGCCTCCGCCGATCTCCTTGAGGCCTGCCGCGGGGGGTCGCGGGCGACGACCGGAAGGAGAGCTGTCATACGTTTTGTATTGTTTTTGAAGCAGATAGCAAGATGGCACgg > 1:578496/1‑150 (MQ=255) gcCGGCGCCGGTCGCGAGCTGCGAACGTAATCTATTAGACGCCCTTCTTTTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCcgc < 2:457984/150‑1 (MQ=255) ggTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGAt < 2:266320/150‑1 (MQ=255) aGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTa > 2:380838/1‑150 (MQ=255) gcgcTGAGCACGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCGGGAGGCGGAGCTGGCATGAGTTTTGTATTGTTTTCCCCGCAGAGAGCAACATAGCACGGTCAGGCCGCGCCTCTGATCTGTaa < 2:26054/150‑1 (MQ=255) cTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACaa < 1:534853/150‑1 (MQ=255) cGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTAc < 1:337145/150‑1 (MQ=255) gaatgaGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAAt < 2:473154/150‑1 (MQ=255) gagGCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGtttt > 2:533820/1‑79 (MQ=38) gagGCCCGTCACATGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGtttt < 1:533820/79‑1 (MQ=255) agGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGc > 2:399010/1‑150 (MQ=255) agGCCCGTCACATGGCGGGCCTTGTTTTGGGGCGTGCGACTGCCGGAAGCCGAGCTGGCGTACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATCGCACGGCCAGCCCTCGTCTCTGATCTTTAACAATACCTACGTAAATTAGCCGc > 2:163958/1‑150 (MQ=255) ccGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTCCGTAAATTAGCCGCTCAc > 1:118583/1‑150 (MQ=255) ccGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCAc < 1:37688/150‑1 (MQ=255) cGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTaa > 2:273139/1‑122 (MQ=255) cGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTaa < 1:273139/122‑1 (MQ=255) gTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACgg < 1:399010/150‑1 (MQ=255) | GCAAGGATCTTCCCCGACCATGAGGTGGTGATGATCCCGGGCCGCGAGCTGCTCTTGGGTGGCGGCAACATTCACTGCCTGACCCAACAGCAGCCGGCGCCGGTCAAGCGCTGAGCCCGTAATGAATGAGAGGCCCGTCATGTGACGGGCCTCTTTTTTGCGCGGGCGACGCCCGGAAGCCGAGCTGGCATACGTTTTGTATTGTTTTTCAAGCAGATAGCAAGATGGCACGGTCAGGCCGCGCCTCTGATCTGTAACAATACCTACGTAAATTAGCCGCTCACGG > NC_002947/321602‑321887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |