| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 459,913 | A→G | 9.1% | intergenic (‑56/+23) | pqqB ← / ← pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 459,913 | 0 | A | G | 9.1% | 120.4 / 2.7 | 44 | intergenic (‑56/+23) | pqqB/pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Reads supporting (aligned to +/- strand): ref base A (19/21); new base G (2/2); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
ATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAG > NC_002947/459771‑460044 | aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGa > 1:59124/1‑150 (MQ=255) tCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGCGTAATATGGCCACTCTCTATGCAGAAACCGGATCGAACAACAGAAAACGCCCCGGCAGGCCGAGGCGTGaa > 1:13471/1‑150 (MQ=255) gTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCt > 1:698771/1‑150 (MQ=255) cTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCt > 2:284509/1‑150 (MQ=255) ttGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTg > 1:24569/1‑150 (MQ=255) gcagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCTCGGCCTGCCGGGGCGttttcgt < 2:377536/130‑7 (MQ=255) gcagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCTCGGCCTGCCGGGGCGttttcgt > 1:377536/1‑124 (MQ=255) cagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGTCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 1:28069/150‑1 (MQ=255) gttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTa > 1:127540/1‑150 (MQ=255) gttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAAACGAAACGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTa < 2:13471/150‑1 (MQ=255) ttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCTCGGCCTGCCGGGGCGtt < 2:450429/115‑2 (MQ=255) ttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCTCGGCCTGCCGGGGCGtt > 1:450429/1‑114 (MQ=255) gggAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCAGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACt > 1:638404/1‑150 (MQ=255) cccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGaa < 2:683125/150‑1 (MQ=255) ccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCTGAGGCGTGAAACCCTAAGCCTGGCTTa > 2:732301/1‑119 (MQ=255) ccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTa < 1:732301/119‑1 (MQ=255) ccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCCGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAg > 2:56522/1‑150 (MQ=255) cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGc < 2:24569/150‑1 (MQ=255) aCCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCGGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa > 1:243901/1‑121 (MQ=255) aCCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 2:243901/121‑1 (MQ=255) gCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt < 1:284509/150‑1 (MQ=255) gCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt < 2:59124/150‑1 (MQ=255) agaACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTAt < 2:127540/150‑1 (MQ=255) ccTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg < 1:751673/150‑1 (MQ=255) ccTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCAGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg < 2:638404/150‑1 (MQ=255) cTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGc > 1:169657/1‑95 (MQ=255) cTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGc < 2:169657/95‑1 (MQ=255) tGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTgg < 2:423844/150‑1 (MQ=255) gTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt < 2:616364/108‑1 (MQ=255) gTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt > 1:616364/1‑108 (MQ=255) tACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt > 1:411304/1‑107 (MQ=255) tACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt < 2:411304/107‑1 (MQ=255) aCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTGTGTGACACATGGaa > 1:153939/1‑150 (MQ=255) tATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCATAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTc > 1:335671/1‑150 (MQ=255) gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGc > 2:745075/1‑108 (MQ=255) gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGc < 1:745075/108‑1 (MQ=255) aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGccag < 1:671849/61‑3 (MQ=255) aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGccag > 2:671849/1‑59 (MQ=255) cGGAGCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGa < 1:75134/150‑1 (MQ=255) ggATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAg < 2:698771/150‑1 (MQ=255) ggATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAACCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAg > 2:248549/1‑150 (MQ=255) ggATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGAGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATgcg > 1:201714/1‑148 (MQ=255) aaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAg < 1:248549/150‑1 (MQ=255) aaacgCCCCCGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAg < 1:56522/150‑1 (MQ=255) | ATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAG > NC_002947/459771‑460044 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |