| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 550,760 | G→A | 45.9% | G33S (GGT→AGT) | rpsJ → | 30S ribosomal protein S10 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 550,760 | 0 | G | A | 45.9% | ‑4.3 / 22.7 | 24 | G33S (GGT→AGT) | rpsJ | 30S ribosomal protein S10 |
| Reads supporting (aligned to +/- strand): ref base G (4/7); new base A (6/4); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
TTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCA > NC_002947/550613‑550896 | ttttAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGt < 1:726711/150‑1 (MQ=255) gCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTTGTGCACAAGTg > 2:220537/1‑150 (MQ=255) gTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTc < 1:504902/150‑1 (MQ=255) aCAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGcc > 1:124262/1‑150 (MQ=255) ccTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTAcc > 1:629483/1‑150 (MQ=255) aTCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGtt < 1:588473/150‑1 (MQ=255) tCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGttt > 1:460786/1‑150 (MQ=255) gCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGt > 1:220089/1‑150 (MQ=255) cAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCATGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGtt < 1:537959/150‑1 (MQ=255) aTCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCa < 1:399595/116‑1 (MQ=255) aTCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCa > 2:399595/1‑116 (MQ=255) cAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACCGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTcaa < 1:383355/150‑1 (MQ=255) ggCTTTCGACCATCGCCTGATCGACCAATGCACCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGCGGTCCAATTTCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCACGCACGTCACCAAAGAcgc > 2:495132/1‑150 (MQ=255) ggCTTTCGACCATCGCCTGATCGACCAATCCTCCCAGGAAATCGTGGAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGAcgc < 1:719588/150‑1 (MQ=255) cATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTTGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAg < 1:220537/150‑1 (MQ=255) cGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTAc < 1:207815/150‑1 (MQ=255) ccTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGAGAACTGTCATCGCGGCCATGCCATTATTCGCATTCTTGTGCGCACCTGCATATAGGGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACga < 2:460786/150‑1 (MQ=255) tGATCGACGAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCATTTCACCGTTCTGGTCTCCCCGCACGGCAACAAAGACGCGCGTGATCAGTACgaga > 2:43665/1‑150 (MQ=255) tGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCAGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCTTGGTCTGGACa > 1:490992/1‑150 (MQ=255) gAAACCGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATc > 1:35014/1‑150 (MQ=255) ccGCGAAACGTACTAGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTcc < 2:629483/150‑1 (MQ=255) cgcgAAACGCACTAGTGCACAAGTGCGTGGTCCACTTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCa < 1:407663/150‑1 (MQ=255) aCGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCAcc < 1:644438/61‑1 (MQ=255) aCGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCAcc > 2:644438/1‑61 (MQ=255) | TTTTAGCGGGCGTAGTGCGTCCGTTGGGAACAGCCTGGAGTCTGAAATCCAATGCAAAATCAGCAAATCCGTATCAGGTTGAAGGCTTTCGACCATCGCCTGATCGACCAATCCACCCAGGAAATCGTGGAAACCGCGAAACGTACTGGTGCACAAGTGCGTGGTCCAATTCCACTGCCTACCCGCAAAGAGCGTTTCACCGTTCTGGTCTCCCCGCACGTCAACAAAGACGCGCGTGACCAGTACGAGATTCGCACTCATAAGCGTGTTCTGGACATCGTCCA > NC_002947/550613‑550896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |