| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 576,552 | G→T | 10.0% | W529L (TGG→TTG) | fdoG → | formate dehydrogenase‑O major subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 576,552 | 0 | G | T | 10.0% | 119.7 / 2.9 | 40 | W529L (TGG→TTG) | fdoG | formate dehydrogenase‑O major subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/17); new base T (2/2); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
TACGCCGCCTACATCGACAAGCGCGCTTCCAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTCACTGCAGCACTGGGCAAGC > NC_002947/576404‑576701 | tACGCCGCCTACATCGACAAGCGCGCTTCCAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTgg > 2:263958/1‑150 (MQ=255) aCATCGACAAGCGCGCTTCCAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAAc < 1:701190/150‑1 (MQ=255) gcTTCCAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGcc > 2:738091/1‑150 (MQ=255) ccAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTacg > 2:889/1‑150 (MQ=255) aaGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTacgac > 1:159836/1‑150 (MQ=255) cTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACTGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGtgctg < 1:721323/150‑1 (MQ=255) tGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTTGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGtt > 2:375148/1‑150 (MQ=255) gCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGCTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCga < 2:411716/150‑1 (MQ=255) gTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACt > 2:246592/1‑105 (MQ=255) gTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACt < 1:246592/105‑1 (MQ=255) ggCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAgg < 1:738091/150‑1 (MQ=255) cTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTTGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGAGCCAGGGCAAAGt < 1:375148/150‑1 (MQ=255) gCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATg < 2:749188/150‑1 (MQ=255) aaGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGt < 1:359433/150‑1 (MQ=255) ttCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTtat > 1:367921/1‑150 (MQ=255) tCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTtata < 1:200953/150‑1 (MQ=255) aGCCTGATGAAGGCCTGGTACGGCGCCAAGGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAg < 1:889/150‑1 (MQ=255) tgatgaAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCt > 2:554586/1‑150 (MQ=255) gatgaAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCtt < 1:210614/150‑1 (MQ=255) gaAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCaa > 1:11863/1‑150 (MQ=255) gaAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCaa < 1:263958/150‑1 (MQ=255) ggCCTGGTACGGCGCCCATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAAccc > 2:442380/1‑150 (MQ=255) gCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGc < 1:601027/141‑1 (MQ=255) gCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACGCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGc > 2:601027/1‑141 (MQ=255) cAATGCCACGGCGGAAAACAACTGGGGTTATGACTTGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGcc > 2:326147/1‑150 (MQ=255) cACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGTCGCGCTGCCGGACaa > 2:521604/1‑150 (MQ=255) ggAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGa > 1:538320/1‑141 (MQ=255) ggAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGa < 2:538320/141‑1 (MQ=255) gAAAACAACTGGGGTTATGACTTGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACcgc < 1:326147/150‑1 (MQ=255) gAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAACCcgc > 2:338485/1‑150 (MQ=255) aacaacTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTc > 1:553558/1‑150 (MQ=255) aacaacTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTc > 2:40856/1‑150 (MQ=255) tGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCAGCACAATAACCGCGTCACTgca > 2:418402/1‑150 (MQ=255) gggTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATc < 2:603998/115‑1 (MQ=255) gggTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATc > 1:603998/1‑115 (MQ=255) ggTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACcgc < 1:664327/138‑1 (MQ=255) ggTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACcgc > 2:664327/1‑138 (MQ=255) gTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGa < 2:508279/127‑1 (MQ=255) gTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGa > 1:508279/1‑127 (MQ=255) cTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTCACt < 2:249749/136‑1 (MQ=255) cTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTCACt > 1:249749/1‑136 (MQ=255) ggCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTCACTGCAGCACTGGGCAAGc < 2:553558/150‑1 (MQ=255) | TACGCCGCCTACATCGACAAGCGCGCTTCCAAGCCGCTGCGGCCTGGGCAGTTGTCGTACTGGCAGAACTACGGCAAGTTCCACGTCAGCCTGATGAAGGCCTGGTACGGCGCCAATGCCACGGCGGAAAACAACTGGGGTTATGACTGGCTGCCCAAACTCGATGTACCGGCCTACGACGTGCTGCGCATGTTCGAGATGATGGGCCAGGGCAAAGTCAATGGTTATATGTGCCAGGGCTTCAACCCGATCGCCGCGCTGCCGGACAAAAACCGCGTCACTGCAGCACTGGGCAAGC > NC_002947/576404‑576701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |