| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 594,198 | A→G | 11.7% | intergenic (‑56/+54) | oprG ← / ← PP_0505 | outer membrane protein OprG/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 594,198 | 0 | A | G | 11.7% | 86.5 / 3.4 | 34 | intergenic (‑56/+54) | oprG/PP_0505 | outer membrane protein OprG/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (12/18); new base G (2/2); total (14/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
CGCAGGATCATGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACCTGCATCTTTCCCTC > NC_002947/594051‑594347 | cgcAGGATCATGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAAc < 2:596791/150‑1 (MQ=255) gATCATGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGaaa > 2:58088/1‑150 (MQ=255) cATGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGtt < 1:99801/150‑1 (MQ=255) aTGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGttt < 2:40398/150‑1 (MQ=255) tCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGaaa > 1:508567/1‑143 (MQ=255) tCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGaaa < 2:508567/143‑1 (MQ=255) cccGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCa < 2:687265/150‑1 (MQ=255) ccGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAATGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAg > 1:96621/1‑150 (MQ=255) gCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGc > 2:465245/1‑150 (MQ=255) gTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGt < 1:465245/150‑1 (MQ=255) tGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAAGGAAGCGTc > 1:516702/1‑150 (MQ=255) cgcAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGtt < 2:296356/150‑1 (MQ=255) aaGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctc < 1:58088/150‑1 (MQ=255) aaGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTtctc > 1:642183/1‑150 (MQ=255) aGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTtc < 2:642183/150‑1 (MQ=255) cTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCt > 2:434434/1‑98 (MQ=255) cTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCt < 1:434434/98‑1 (MQ=255) cATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAGACGGAAAGtt < 2:570915/69‑1 (MQ=255) cATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAGACGGAAAGtt < 2:571278/69‑1 (MQ=255) cATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAGACGGAAAGtt > 1:571278/1‑69 (MQ=255) cATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAGACGGAAAGtt > 1:570915/1‑69 (MQ=255) ggAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGa > 2:101243/1‑69 (MQ=255) ggAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGa < 1:101243/69‑1 (MQ=255) tCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGAc > 2:443258/1‑64 (MQ=255) tCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGAc < 1:443258/64‑1 (MQ=255) ccAAATGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCg < 2:96621/150‑1 (MQ=255) cAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCgt < 2:516702/150‑2 (MQ=255) cGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGc < 2:342509/150‑1 (MQ=255) aaTCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCg > 2:376989/1‑150 (MQ=255) agtagtCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCgctgct > 2:525691/1‑150 (MQ=255) tCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAg > 2:6248/1‑150 (MQ=255) aTGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCttt < 1:6248/150‑1 (MQ=255) gTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGAc < 1:376989/150‑1 (MQ=255) ggTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACCTGCATCttt < 1:680057/150‑1 (MQ=255) aaCGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACCTGCATCTTTCCctc > 1:308697/1‑150 (MQ=255) | CGCAGGATCATGTCCCCCGCCTGATAGGCGTGGGCGGCAGGGGCGGCGAGCGCAAGGGCCACAAGCGAGGCACCCAGCAAGGACTTGTTCATGGAAGCTCCCAAAGGACGTTATTAATCGAGTAGTCCAATGGTACGGAGCTGGTTAAACGGAAAGTTTGACCCAGCTCAACGAAGCGTCTTCACGAATTCGAAACAGTTCTCATCTCAGCTCATAAGCGTAGATTTTCTCGGCTTCCATCTGGTAGCCGGCGTCGGCCAGCTCGCTGCTGGAAGCTTTGACCTGCATCTTTCCCTC > NC_002947/594051‑594347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |