Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 739,393 T→A 9.7% E9V (GAA→GTA) ‡ pilD ← leader peptidase/N‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947739,3930TA 9.7% 109.0 / 4.2 41E9V (GAA→GTA) ‡pilDleader peptidase/N‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base A (2/2);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GTCGGCAGCCCCAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCC  >  NC_002947/739252‑739541
                                                                                                                                             |                                                                                                                                                    
ggcggttgCCTCAATACCGCTGTCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTcagccagc                                                                                                                                              <  2:606445/143‑1 (MQ=255)
      aGCCCCAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGTCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGcc                                                                                                                                        <  1:233610/150‑1 (MQ=255)
         cccAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGAAcagccagcgtattt                                                                                                                                        <  1:218653/147‑7 (MQ=255)
         cccAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGAAcagccagcgtattt                                                                                                                                        >  2:218653/1‑141 (MQ=255)
         cacAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCAGTCGTCTATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCAt                                                                                                                                     >  1:617297/3‑150 (MQ=255)
            aaTACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATaaa                                                                                                                                  <  2:297562/150‑1 (MQ=255)
                  tctTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGAAcagccag                                                                                                                                               <  1:145902/131‑1 (MQ=255)
                  tctTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGAAcagccag                                                                                                                                               >  2:145902/1‑131 (MQ=255)
                    ttGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGtt                                                                                                                          <  2:750804/150‑1 (MQ=255)
                        gcCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACAAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGa                                                                                                                      <  1:420148/150‑1 (MQ=255)
                               gCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACt                                                                                                               >  1:365729/1‑150 (MQ=255)
                                                   aTGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCa                                                                                           <  1:472305/150‑1 (MQ=255)
                                                    tGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAAc                                                                                                        <  2:424350/136‑1 (MQ=255)
                                                    tGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAAc                                                                                                        >  1:424350/1‑136 (MQ=255)
                                                      aTCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCACACAGCAGGCCGAGCACCGTGGCACGGGTGAGTAATTTTTCTGGCTGTTCTGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGt                                                                                        <  2:84031/150‑1 (MQ=255)
                                                         ggCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCaaa                                                                                                                 <  1:556298/122‑1 (MQ=255)
                                                         ggCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCaaa                                                                                                                 >  2:556298/1‑122 (MQ=255)
                                                              gCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCATCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGcc                                                                                >  2:24068/1‑150 (MQ=255)
                                                                     acCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGcca                                                                               <  1:189398/144‑1 (MQ=255)
                                                                     acCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGcca                                                                               >  2:189398/1‑144 (MQ=255)
                                                                      ccAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAg                                                                        <  1:250845/150‑1 (MQ=255)
                                                                             gTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGccaccc                                                                 >  1:180570/1‑150 (MQ=255)
                                                                                gATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTg                                                                                                      <  2:449015/110‑1 (MQ=255)
                                                                                gATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTg                                                                                                      >  1:449015/1‑110 (MQ=255)
                                                                                           ccaccaGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGccc                                                     <  2:574399/148‑1 (MQ=255)
                                                                                           ccaccaGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGccc                                                     >  1:574399/1‑148 (MQ=255)
                                                                                             accaGCAGGCCGAGCACCGCGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCAAAAAGTCATGTTCACATCAAACTACCCAACTAGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGa                                                 >  2:471283/1‑150 (MQ=255)
                                                                                              ccaGCAGGCCGAGCACCGTGGCAAGGGTGAGGCAATAAGGTAAAAAATATCCTAGCAAAGGCCGTAAAGTGATGCTCAGCAAAAACTACACAACTACAAGATCGGCAGGTTCATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGAt                                                >  1:578589/1‑150 (MQ=255)
                                                                                                 gcagGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATGGGCAGGTACATCGCCACCACCAGGCCAGCCAACAGCAAGCCCAGGATAAg                                             >  1:369506/1‑150 (MQ=255)
                                                                                                   agGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCa                                           <  1:668853/150‑1 (MQ=255)
                                                                                                       cGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGAt                                       >  1:465905/1‑150 (MQ=255)
                                                                                                        gAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGccacccac                                                               <  1:489828/125‑1 (MQ=255)
                                                                                                        gAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGccacccac                                                               >  2:489828/1‑125 (MQ=255)
                                                                                                          gCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATgga                                    >  1:48881/1‑149 (MQ=255)
                                                                                                           cACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGcc                                   <  2:465905/150‑1 (MQ=255)
                                                                                                                  gCAAGGGTGCGGAAGTAGGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCca                            <  2:578589/150‑1 (MQ=255)
                                                                                                                       ggTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAgg                       >  1:434497/1‑150 (MQ=255)
                                                                                                                          gAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTg                    <  2:533427/150‑1 (MQ=255)
                                                                                                                                aTACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAAc              >  1:143313/1‑150 (MQ=255)
                                                                                                                                  aCGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTg            >  2:320296/1‑150 (MQ=255)
                                                                                                                                     ctggctgTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATc         <  2:180570/150‑1 (MQ=255)
                                                                                                                                            ttCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGcc  >  1:728897/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GTCGGCAGCCCCAATACCTCTTGCGCCTCACGCTGCCATTGCCGCTCCAGCATGATCGGCAGGCGATACACCAGCACGTTGATAAAGCTGCCCACCAGCAGGCCGAGCACCGTGGCAAGGGTGAGGAAATACGCTGGCTGTTCAGCCAGCAAAGCCCATAAAGTCATGTTCAGATCAAACTACCCAACTGGAAGATCGGCAGGTACATCGCCACCACCAGGCCACCCACCAGCAAGCCCAGGATCAGCACGATGGCCGGCTCCAGCAGGCTGGTCAACTGATCCAGCGCC  >  NC_002947/739252‑739541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑