| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 743,463 | A→G | 6.6% | intergenic (+139/+135) | PP_0635 → / ← PP_0636 | group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 743,463 | 0 | A | G | 6.6% | 182.0 / 2.3 | 60 | intergenic (+139/+135) | PP_0635/PP_0636 | group II intron‑encoding maturase/cold shock DNA‑binding domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (27/29); new base G (2/2); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
GCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATT > NC_002947/743314‑743610 | gcgTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGa < 2:140681/150‑1 (MQ=255) ccTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGAcacaca < 1:533258/150‑1 (MQ=255) ccTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGATAGGTGAAGCAACCTTCGGTTGCGTTACATAGCGGAcacaca > 1:498994/1‑150 (MQ=255) cTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAg > 1:490147/1‑150 (MQ=255) aCGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGcc > 1:644843/1‑150 (MQ=255) cGGAACCGTACGCACGGTTGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCGGCCTTGTGTGCCCGCTAGGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTTACTTGCCg < 2:295960/150‑1 (MQ=255) aaCCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCgggg < 1:688525/150‑1 (MQ=255) cACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACtt < 1:212224/150‑1 (MQ=255) aCGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTg < 1:561689/150‑1 (MQ=255) cGGTGGTTTTAGAGGACGGCGGGTGTGCCCCAGACTCCTCCCCGCTTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTgg < 2:498994/150‑1 (MQ=255) tgAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCgcggcg > 2:53171/1‑150 (MQ=255) agGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGtgtg > 1:3177/1‑150 (MQ=255) ggACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCCTTATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTgg < 1:196388/150‑1 (MQ=255) cggcggGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGc < 2:644843/150‑1 (MQ=255) ggcggGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGt < 2:280406/134‑1 (MQ=255) ggcggGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTGCTGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGt > 1:280406/1‑134 (MQ=255) gcggGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCag > 2:57545/1‑150 (MQ=255) gggTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGAagag > 2:166529/1‑150 (MQ=255) gtgtGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCag > 1:515284/1‑146 (MQ=255) gtgtGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCag > 1:515358/1‑146 (MQ=255) gtgtGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCac < 2:515284/146‑2 (MQ=255) gtgtGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCac < 2:515358/146‑2 (MQ=255) aTCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAgg > 2:23422/1‑150 (MQ=255) gCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAg < 1:397340/150‑1 (MQ=255) tCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAg > 1:139338/1‑150 (MQ=255) gATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGtc < 2:501796/150‑2 (MQ=255) ttGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGc < 2:718747/150‑1 (MQ=255) gcgAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCggg < 2:81123/89‑1 (MQ=255) gcgAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCggg > 1:81123/1‑89 (MQ=255) gAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACATCGTCGTGACTTGCCGGGGAAACTATGTGGTCGTGGCGTGGGGAGCAGAGGGTGGAGGGGGGGGGTTACAGGGGCGGttct > 2:345171/1‑148 (MQ=255) aaTCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGAAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCaa < 1:166529/150‑1 (MQ=255) aaTCAGACCTGACTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCaa < 2:227967/150‑1 (MQ=255) tCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAg < 2:374006/110‑1 (MQ=255) tCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAg > 1:374006/1‑110 (MQ=255) gACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCaa < 1:603169/145‑1 (MQ=255) gACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCaa > 2:603169/1‑145 (MQ=255) cTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCtt > 1:560405/1‑150 (MQ=255) cTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCtt > 1:315792/1‑150 (MQ=255) tGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCa < 1:317203/105‑1 (MQ=255) tGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCa > 2:317203/1‑105 (MQ=255) gacgctgtgtgTCCGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTg < 2:338649/145‑1 (MQ=21) gacgctgtgtgTCCGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTg > 1:338649/6‑150 (MQ=21) ccTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGc < 1:23422/150‑1 (MQ=255) ttGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAg > 2:450705/1‑111 (MQ=255) ttGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAg < 1:450705/111‑1 (MQ=255) gCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCTTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGc < 1:12655/150‑1 (MQ=255) tccGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCaga < 1:740279/95‑1 (MQ=17) tccGCTATGTAACGCAACCGAAGGTTGCTTCACATAGCGGGCACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCaga > 2:740279/2‑96 (MQ=17) ccGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTa > 1:96863/1‑150 (MQ=255) tgAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGt > 1:367243/1‑150 (MQ=255) ttCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGt < 2:319615/93‑1 (MQ=255) ttCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGt > 1:319615/1‑93 (MQ=255) tCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGa < 2:3177/150‑1 (MQ=255) gTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGt < 2:239317/89‑1 (MQ=255) gTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGAATGTAGGGGAGCGt > 1:239317/1‑89 (MQ=255) aTAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTa > 2:278396/1‑70 (MQ=255) aTAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTa < 1:278396/70‑1 (MQ=255) aTAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGAc > 2:170290/1‑150 (MQ=255) ggACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGa > 2:642509/1‑119 (MQ=255) ggACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGa < 1:642509/119‑1 (MQ=255) ggACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCAtt < 1:57545/150‑1 (MQ=255) | GCGTAACCTGAACCGCCGTGTACGGAACCGTACGCACGGTGGTGTGAGAGGACGGCGGGTGTGAATCCGCCTCCTACTCGATTGCGCGAAATCAGACCTGCCTTGTGTGCCCGCTATGTGAAGCAACCTTCGGTTGCGTTACATAGCGGACACACAGCGTCGTGACTTGCCGGGGAAACTACTTGGTCGCGGCGTGTGGAGCAGAGACTGTAGGGGAGCGTTGACAGTGGCGGTTTCAAGCTAGCTTGGCGGTCACGCTAAGCCGAGTGAGGCATTCGATCCTGCTAGCGACGCATT > NC_002947/743314‑743610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |