| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 811,444 | G→A | 12.1% | Q271* (CAG→TAG) ‡ | PP_0698 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 811,444 | 0 | G | A | 12.1% | 79.1 / 3.7 | 33 | Q271* (CAG→TAG) ‡ | PP_0698 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (20/9); new base A (2/2); total (22/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
TACCCATCAGGCCGTATCCATTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCAGGCCCAGTCCGGCG > NC_002947/811302‑811592 | gaCCCATCAGGCCGTATCCATTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTg > 2:517013/2‑150 (MQ=255) cATTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGc < 2:569826/150‑1 (MQ=255) aTTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCCAGCGCttcgc < 1:696685/133‑4 (MQ=255) aTTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCCAGCGCttcgc > 2:696685/1‑130 (MQ=255) aTTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACACGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCt > 1:532078/1‑150 (MQ=255) tttGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTg > 1:335908/1‑150 (MQ=255) cgccgGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCCAGCGCttc > 2:555829/1‑120 (MQ=255) cgccgGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCCAGCGCttc < 1:555829/121‑2 (MQ=255) gCACCTGGGGTGGTGATGGCGGTGCGTGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAgg > 1:101417/1‑150 (MQ=255) gCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAgg < 2:335908/150‑1 (MQ=255) tggtgATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTg > 2:461356/1‑93 (MQ=255) tggtgATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTg < 1:461356/93‑1 (MQ=255) gggggCGTCGTTCTGTATCAACCCAAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGaa < 2:532078/150‑1 (MQ=255) gCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGgc > 1:738843/1‑150 (MQ=255) gtcgtTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCTGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGgcgc > 1:545097/1‑150 (MQ=255) tCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCa < 2:101417/150‑1 (MQ=255) tCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGcc > 2:129460/1‑150 (MQ=255) aaCCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCg > 2:476725/1‑150 (MQ=255) cccAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGc < 2:500616/64‑1 (MQ=255) cccAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGc > 1:500616/1‑64 (MQ=255) gCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTc < 2:557932/143‑1 (MQ=255) gCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTc > 1:557932/1‑143 (MQ=255) aGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTtcgtcg > 2:484727/1‑150 (MQ=255) ttCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCAcc < 1:476725/150‑1 (MQ=255) aTTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAggg > 2:371552/1‑150 (MQ=255) aTTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAggg > 1:250458/1‑150 (MQ=255) gTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGa > 1:495532/1‑150 (MQ=255) tCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGGCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATgg > 1:534354/1‑150 (MQ=255) aCTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGgcgc > 1:250313/1‑94 (MQ=255) aCTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGgcgc < 2:250313/94‑1 (MQ=255) aCTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCa > 2:432150/1‑150 (MQ=255) aCTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCa > 1:684780/1‑150 (MQ=255) tCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCAgg < 1:484727/150‑1 (MQ=255) gAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCAGGCCCAg > 2:56302/1‑150 (MQ=255) tGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCAGGCCCAGTCCGgcg > 2:719286/1‑150 (MQ=255) | TACCCATCAGGCCGTATCCATTTGGATACCGCCGGTTATCGCACCTGGGGTGGTGATGGCGGTGCGGGGGCGTCGTTCTGTATCAACCCCAAATGAGCCAGGCACTTCCGGCATTCGTCCATTATCCACTCGGCGAAGCGCTGGCGCTTGCCGCTGTCGGCCAGAGGCTGCGGGCTGAGCAGGTGGTAGGCCGAGCCATCGCGCACAAAACCGAACGGCGCCTGCAGCTGCCCGCTGTCGAGTTCGTCGCGCACCATCAGGGCTGAGGCAATGGCCAGGCCCAGTCCGGCG > NC_002947/811302‑811592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |