| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 949,560 | G→A | 13.8% | P133P (CCG→CCA) | dsbB → | disulfide bond formation protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 949,560 | 0 | G | A | 13.8% | 66.9 / 3.8 | 29 | P133P (CCG→CCA) | dsbB | disulfide bond formation protein B |
| Reads supporting (aligned to +/- strand): ref base G (13/12); new base A (2/2); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
ACTCGCCAACCCCATGGTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGAATTGCATGGGGTCTTTTG > NC_002947/949443‑949699 | aCTCGCCAACCCCATGGTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCa > 1:346046/1‑150 (MQ=255) cccATGGTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACtt < 1:134649/150‑1 (MQ=255) ccATGGTCAGTTGCGGTATCGATACCCTGCAGCCGCTCGTGGATGACCTGCCACTGGCAAAGCTGTGACCACTGCCGTTCCAGGTCGACGACTTCTGCAGCACGCCGTATCCCCCGATCCGCGCACTGTCCCTGCCGCAATGACCACTtg > 2:34455/1‑150 (MQ=255) ccATGGTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTtg < 1:79576/150‑1 (MQ=255) ggTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCAACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGc < 2:739819/150‑1 (MQ=255) cAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGtt < 2:749513/150‑1 (MQ=255) gCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGc < 1:333412/150‑1 (MQ=255) ggTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCAGCCGATTCTCAGGCTCTCGCTGTCGCAATGGGCACTTGTGGCGTTCGTGCTg > 2:458438/1‑150 (MQ=255) aTACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGAccgccg > 1:227046/1‑150 (MQ=255) ccTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTg > 1:638823/1‑109 (MQ=255) ccTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTg < 2:638823/109‑1 (MQ=255) tGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTtgtg < 2:269963/125‑1 (MQ=255) tGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTtgtg > 1:269963/1‑125 (MQ=255) aCTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCg > 1:750969/1‑150 (MQ=255) gCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGc < 2:346046/150‑1 (MQ=255) aaaGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCATATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCa > 1:233859/1‑150 (MQ=255) aaaGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCATATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCa > 1:233589/1‑150 (MQ=255) aGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAgg > 1:474923/1‑150 (MQ=255) ccACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGt < 2:257246/104‑1 (MQ=255) ccACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGt > 1:257246/1‑104 (MQ=255) tGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTtgtg > 1:744822/1‑80 (MQ=255) tGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTtgtg < 2:744822/80‑1 (MQ=255) tGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAg > 1:11393/1‑150 (MQ=255) tCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGAATTGCa < 2:227046/150‑1 (MQ=255) ggCTTCTGCAGCACGCCATATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGGATTGCATgggg < 2:233859/150‑1 (MQ=255) ggCTTCTGCAGCACGCCATATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGAATTGCATgggg < 2:233589/150‑1 (MQ=255) cTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTccc < 2:625034/135‑1 (MQ=255) cTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTccc > 1:625034/1‑135 (MQ=255) gcagcaCGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGAATTGCATGGGGTCTTTTg > 1:69151/1‑150 (MQ=255) | ACTCGCCAACCCCATGGTCAGTTGCGGTATCGATACCCTGCAGCCGATCGTGGATGACCTGCCACTGGCAAAGCTGTGGCCACTGGCGTTTCAGGTCGACGGCTTCTGCAGCACGCCGTATCCGCCGATCCTCGGCCTGTCGCTGGCGCAATGGGCACTTGTGGCGTTCGTGCTGACCGCCGTCCTGGTCCCGCTCGGTATCTACCGCAATCGACGCCAGGCTTAGACAAAAGTCCCGAATTGCATGGGGTCTTTTG > NC_002947/949443‑949699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |