New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	5257895 =	34 (1.180)	6 (0.220)	5/246	2.9	13.1%	intergenic (‑46/+98)	PP_4634/PP_4635	alpha/beta family hydrolase/trans‑2‑enoyl‑CoA reductase
	?	NC_002947	5257986 =	48 (1.780)					intergenic (‑137/+7)	PP_4634/PP_4635	alpha/beta family hydrolase/trans‑2‑enoyl‑CoA reductase
Rejected: Frequency below/above cutoff threshold.

GCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTCGAACAGG  >  NC_002947/5257841‑5258122                                                                                                                                                  |                                                                                                                                         gCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGGGAAGAGGCCGGTAGAGGGTTAccc                                                                                                                                      >  1:65158/1‑150 (MQ=255)        aTGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACa                                                                                                                               <  2:199959/150‑1 (MQ=255)                            gtgCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTc                                                                                                           <  2:129351/150‑1 (MQ=255)                             tgCGACAAAACTAGCAAAGATTTTGCAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGTGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACACCTCGACGCAGTCGAACTTca                                                                                                          <  2:287634/150‑1 (MQ=255)                                     aaCTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTg                                                                                                  <  2:380651/150‑1 (MQ=255)                                     aaCTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTg                                                                                                  <  2:434726/150‑1 (MQ=255)                                        tAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGcc                                                                                               >  2:165291/1‑150 (MQ=255)                                           cAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGTGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGGGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACg                                                                                            >  2:685505/1‑150 (MQ=255)                                                 ttttGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGAt                                                                                                                                   <  1:22388/105‑1 (MQ=255)                                                 ttttGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGAt                                                                                                                                   >  2:22388/1‑105 (MQ=255)                                                    tGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCg                                                                                   <  1:685522/150‑1 (MQ=255)                                                           tgtaccggcctcttcgcgggcaagcccgctcccacaggttctccccctgtaggagcgggttcacccgcgaagaggccagtacaggctTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCaa                                                                            >  2:178144‑M2/88‑150 (MQ=255)                                                                  gCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTGGTAGTCAACGTCAgc                                                                     >  2:507231/1‑150 (MQ=255)                                                                     tctTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAgcgcg                                                                  >  1:320628/1‑150 (MQ=255)                                                                          gcgGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGCAGAGGCCGGTACAGGCTTACCCGAGTACAGCTCGACGCAGCCGAACTGCGCATCGGTGCGCACGGCGTTGACTTGTTCAGCGTCCGCGCGCCCGa                                                             >  2:152866/1‑150 (MQ=25)                                                                              gcaagcccgctcccacaggttctccccctgtaggagcgggttcacccgcgaagaggccagtacaggctTACCCGATTACAGCTCGACGCAGTc                                                                                                                  >  1:214689‑M2/69‑93 (MQ=255)                                                                              gcaagcccgctcccacaggttctccccctgtaggagcgggttcacccgcgaagaggccagtacaggctTACCCGATTACAGCTCGACGCAGTc                                                                                                                  <  2:214689‑M2/25‑1 (MQ=255)                                                                                   cccgctcccacaggttctccccctgtaggagcgggttcacccgcgaagaggccggtacaggctTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACa                                                    >  2:386036‑M2/64‑150 (MQ=255)                                                                                   cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTGCAGCTCGACGCAGTAGAAATTCACATCGTGGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACa                                                    >  2:98814/1‑150 (MQ=39)                                                                                   cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTg                                                                                                  >  2:315275/1‑104 (MQ=255)                                                                                   cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACa                                                    >  2:499508/1‑150 (MQ=255)                                                                                   cccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCCGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTg                                                                                                  <  1:315275/104‑1 (MQ=255)                                                                                   cccGCTCCTACAGGGGGAGAACCTGCGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACa                                                    >  1:549924/1‑150 (MQ=255)                                                                                    ccGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAg                                                   >  1:249455/1‑150 (MQ=255)                                                                                      gCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGa                                                                                                                                    <  2:171471/67‑1 (MQ=255)                                                                                      gCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGa                                                                                                                                    >  1:171471/1‑67 (MQ=255)                                                                                       cTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGtt                                                <  2:249455/150‑1 (MQ=255)                                                                                        tCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGGAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTg                                               >  2:714160/1‑150 (MQ=255)                                                                                            aCAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGAAAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGa                                           >  1:4159/1‑150 (MQ=255)                                                                                                 gggAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGc                                      <  1:714160/150‑1 (MQ=255)                                                                                                  ggAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGc                                                                                                >  1:638420/1‑91 (MQ=255)                                                                                                  ggAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGc                                                                                                <  2:638420/91‑1 (MQ=255)                                                                                                      cccctgtaggagcgggttcacccgcgaagaggccggtacaggctTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGcttctt                                 <  1:386036‑M2/106‑1 (MQ=255)                                                                                                      cccctgtaggagcgggttcacccgcgaagaggccagtacaggctTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGcttctt                                 <  1:178144‑M2/106‑1 (MQ=255)                                                                                                        ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAgg                                                  <  1:4993/131‑1 (MQ=255)                                                                                                        ccTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAgg                                                  >  2:4993/1‑131 (MQ=255)                                                                                                            tgGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGa                                              >  2:355819/1‑131 (MQ=255)                                                                                                            tgGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGa                                              <  1:355819/131‑1 (MQ=255)                                                                                                             gggAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGt                                                                                  >  1:562172/1‑94 (MQ=255)                                                                                                             gggAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGt                                                                                  <  2:562172/94‑1 (MQ=255)                                                                                                                 gCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTTGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGt                      >  2:668011/1‑150 (MQ=255)                                                                                                                  cGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTa                     <  1:165291/150‑1 (MQ=255)                                                                                                                     gCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAg                                             >  2:358225/1‑123 (MQ=255)                                                                                                                     gCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAg                                             <  1:358225/123‑1 (MQ=255)                                                                                                                     gCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTagtc                  >  2:697118/1‑150 (MQ=255)                                                                                                                       ttGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTagtcag                >  1:37254/1‑150 (MQ=255)                                                                                                                           ccGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGGCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGACGCCGAACCGGTTGAGGACCTGCTTCTTGTAACCGGCGTAGtcagtcag            <  1:98814/150‑1 (MQ=255)                                                                                                                           ccGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGtcagtcag            >  2:118657/1‑150 (MQ=255)                                                                                                                            cgcgAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGc           <  1:174046/150‑1 (MQ=255)                                                                                                                             gcgAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCt          <  2:320628/150‑1 (MQ=255)                                                                                                                              cgAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTc         <  2:542093/150‑1 (MQ=255)                                                                                                                                   agGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTCGAACa    >  2:524406/1‑150 (MQ=255)                                                                                                                                    ggCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTCGAACAg   <  2:37254/150‑1 (MQ=255)                                                                                                                                     gCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTCGAACAgg  <  1:499508/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                         GCTACTCATGGCGCATGTCCTGTTGTTGTGCGCCAAAACTAGCAAAGATTTTGTAGCCTGTACTGGCCTCTTCGCGGGTGAACCCGCTCCTACAGGGGGAGAACCTGTGGGAGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGCTTACCCGATTACAGCTCGACGCAGTCGAACTTCACATCGGTGGCCACGTCTTTGTCGTAGTCAACGTCAGCGCGCTCGAAGCCGAACAGGTTGAGGAACTGCTTCTTGTAACCGGCGTAGTCAGTCAGCTCGAACAGG  >  NC_002947/5257841‑5258122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.