Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,327,1270CT60.0% 8.9 / 42.3 45intergenic (+97/‑196)PP_16SE/PP_23SE16S ribosomal RNA/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (8/10);  new base T (13/14);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTA  >  NC_002947/1326978‑1327229
                                                                                                                                                     |                                                                                                      
tGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGc                                                                                                        >  2:2741/1‑150 (MQ=32)
   aGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATc                                                                                                     >  1:87439/1‑150 (MQ=17)
    gTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCg                                                                                                    >  1:8427/1‑150 (MQ=17)
          aCAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGtt                                                                                              <  1:657771/150‑1 (MQ=17)
          aCAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGtt                                                                                              >  1:52458/1‑150 (MQ=32)
          aCAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGtt                                                                                              <  2:32477/150‑1 (MQ=32)
               gTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGAttt                                                                                         <  1:234461/150‑1 (MQ=17)
                   ccGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGAttttttc                                                                                     <  1:502858/150‑5 (MQ=17)
                     gTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGt                                                                                               >  2:139015/1‑138 (MQ=18)
                     gTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGt                                                                                               <  1:139015/138‑1 (MQ=18)
                        ggggAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCTACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCtttt                                                                                <  2:459141/150‑1 (MQ=17)
                        ggggAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCtttt                                                                                <  2:52458/150‑1 (MQ=32)
                           gAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACCAGAATTGCTTGCACGTTATTCGAATTCGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTc                                                                             >  1:632458/1‑150 (MQ=11)
                                   ggCTGGACCACCTCCTTAATCGACGACATCAGCCTGGAGTTGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGtt                                                                     <  1:514371/150‑1 (MQ=255)
                                    gCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTc                                                                    <  2:649331/150‑1 (MQ=17)
                                      tGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCtt                                                                  <  2:87439/150‑1 (MQ=17)
                                       ggATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACAAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCttt                                                                 <  1:520785/150‑1 (MQ=17)
                                              ctcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAAtt                                                          >  2:194585/1‑150 (MQ=17)
                                               tcctTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTc                                                         <  2:416705/150‑1 (MQ=17)
                                                  ttAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGa                                                      >  1:278942/1‑150 (MQ=17)
                                                    aaTCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGata                                                    <  2:508726/150‑1 (MQ=17)
                                                    aaTCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTAATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGata                                                    >  2:363091/1‑150 (MQ=12)
                                                          cgacATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgat                                              <  2:278942/150‑1 (MQ=17)
                                                          cgacATCAGCCTGCTGATGAGCTCCCACACGAAGTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgat                                              <  2:406005/150‑1 (MQ=17)
                                                              aTCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTgatagat                                          >  1:192525/1‑150 (MQ=17)
                                                               tCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAAt                                                                                                 <  1:699750/94‑1 (MQ=18)
                                                               tCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAAt                                                                                                 >  2:699750/1‑94 (MQ=21)
                                                                     tgctgATGAGCTCCCACACGAATTGCTTGATTCATAGTAGAAGAAGTTTATCACCGCATAAACCTGGAAATGAACATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACt                                   >  1:633479/1‑150 (MQ=11)
                                                                        tgatgaGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGaa                                <  2:37344/150‑1 (MQ=17)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGCACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTCTAAAAATTCGGa                                                      <  1:414746/120‑1 (MQ=12)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTgg                                                                                     >  2:547201/1‑89 (MQ=25)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTgg                                                                                     <  1:547201/89‑1 (MQ=25)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCtttt                                                                                <  1:482777/94‑1 (MQ=34)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCtttt                                                                                >  2:482777/1‑94 (MQ=33)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATc                                                                        <  2:429506/102‑1 (MQ=33)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATc                                                                        >  1:429506/1‑102 (MQ=33)
                                                                                tCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTCTAAAAATTCGGa                                                      >  2:414746/1‑120 (MQ=18)
                                                                                 cccACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGttt                       <  2:632458/150‑1 (MQ=32)
                                                                                  ccACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTc                                                                             <  1:263083/95‑1 (MQ=21)
                                                                                  ccACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTc                                                                             >  2:263083/1‑95 (MQ=21)
                                                                                     cacGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCAct                   <  2:255316/150‑1 (MQ=17)
                                                                                          aTTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtgg              >  2:240121/1‑150 (MQ=17)
                                                                                          aTTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtgg              >  1:244718/1‑150 (MQ=32)
                                                                                              cTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa          >  2:464162/1‑150 (MQ=32)
                                                                                               ttGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGAt         >  1:449667/1‑150 (MQ=17)
                                                                                                      aTTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGTATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTa  >  1:8785/1‑150 (MQ=17)
                                                                                                                                                     |                                                                                                      
TGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTAATCGACGACATCAGCCTGCTGATGAGCTCCCACACGAATTGCTTGATTCATTGTCGAAGACGTTTTTCCTGGTCAGAACCTGGAAATGAGCATTCGCATCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTA  >  NC_002947/1326978‑1327229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑