| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,458,940 | 1 | . | A | 41.7% | 0.1 / 19.3 | 12 | intergenic (+17/‑45) | leuS/PP_4795 | leucine‑‑tRNA ligase/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base . (3/4); new base A (2/3); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAA > NC_002947/5458791‑5459082 | tgccGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGc < 2:310642/150‑1 (MQ=255) cgAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGCAATCGA‑TGCGGGTgg < 1:328521/150‑1 (MQ=255) agcaACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGAAGGGTCACCGTGGGACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGcaa < 2:218578/150‑1 (MQ=255) aTCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCt < 1:87404/150‑1 (MQ=255) aCCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCt > 2:316364/1‑150 (MQ=255) gggCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGtt < 1:273326/150‑1 (MQ=255) aaCTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCAc > 2:355851/1‑150 (MQ=255) gCCCACCCGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCa > 1:8318/1‑100 (MQ=39) gCCCACCCGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCa < 2:8318/100‑1 (MQ=39) ccACCCGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGgc < 1:48193/130‑1 (MQ=255) ccACCCGCAATCGA‑TGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGgc > 2:48193/1‑130 (MQ=255) ccACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa < 1:355851/150‑1 (MQ=255) ccACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGaa > 1:86975/1‑150 (MQ=255) | TGCCGCTGCCAGCCGCGAAGAAGTCGAAGCCGCTGCCCGCAGCAACGAGAATGTCCTGCGCTTCATCGATGGCCTGACCATCCGCAAGGTCATCGTGGTACCGGGCAAGCTGGTCAACATCGTCGCCAACTGATGAAAACGCCCACCCGC‑ATCGATTGCGGGTGGGCGGAACAGGCCCAAAAGGGAGCAACAACATGATCAAACGCAATTTGCTGGTAATCGGCCTGGCGGTCATGCTCAGCGCCTGCGGTTTCCAGCTGCGCGGCACCGGCGCCACCGAGCTGAGCGTGAA > NC_002947/5458791‑5459082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |