Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	58,750	0	T	A	35.3%	13.2 / 11.6	17	intergenic (‑661/+27)	PP_5424/PP_0050	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (6/5);  new base A (4/2);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCAGTC  >  NC_002947/58605‑58899                                                                                                                                                  |                                                                                                                                                      gTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCgg                                                                                                                                                   >  2:131173/1‑150 (MQ=255)           ctcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGt                                                                                                                                         <  1:304455/150‑1 (MQ=255)            tcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCGGCTGCTGCGGGCTTTTTAGTg                                                                                                                                        >  2:322959/1‑150 (MQ=255)                       gtgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACaa                                                                                                                             >  1:375034/1‑150 (MQ=255)                        tgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGTGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAAt                                                                                                                            <  1:131173/150‑1 (MQ=255)                                    cTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTg                                                                                                                >  2:270508/1‑150 (MQ=255)                                         tttCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAAt                                                                                                           <  1:270508/150‑1 (MQ=255)                                                                  ccAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATg                                                                                  <  2:375034/150‑1 (MQ=255)                                                                                            ccTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcac                                                        >  2:215103/1‑150 (MQ=255)                                                                                                                      aTTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca                                                       >  1:277573/1‑125 (MQ=255)                                                                                                                      aTTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca                                                       <  2:277573/125‑1 (MQ=255)                                                                                                                               gcacTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgc                            >  2:191474/2‑143 (MQ=255)                                                                                                                               gcacTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgc                            <  1:191474/142‑1 (MQ=255)                                                                                                                                   tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCCATCACGGTATGTACCAGAACAGAGTCGCCATTCACATAACGCAAACGTTTCTCACGAGGTGGCGCATACAGGTg                 >  1:113241/1‑150 (MQ=255)                                                                                                                                   tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAACCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAAt                                                                                   >  1:58724/1‑84 (MQ=37)                                                                                                                                   tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAACCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAAt                                                                                   <  2:58724/84‑1 (MQ=37)                                                                                                                                     aaaaGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgc               >  2:279058/1‑150 (MQ=255)                                                                                                                                                  tgcGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGACGATCACGAGGTGGCCCATACACCTGGCCAGCCTTCcagtc  >  2:263851/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                      GTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCAGTC  >  NC_002947/58605‑58899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑