Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947322,1420TC42.5% 13.3 / 39.8 40intergenic (+427/+31)aguA/PP_0267agmatine deiminase/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base T (9/14);  new base C (10/7);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.70e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321999‑322291
                                                                                                                                               |                                                                                                                                                     
gCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                                 <  1:572440/150‑1 (MQ=255)
gCATCAGGGGCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                                 <  2:694950/150‑1 (MQ=255)
  aTCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAaga                                                                                                                                               <  1:10320/150‑1 (MQ=255)
   tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAagag                                                                                                                                              <  1:542212/150‑1 (MQ=255)
   tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAag                                                                                                                                                <  2:817504/148‑1 (MQ=255)
   tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAag                                                                                                                                                >  1:817504/1‑148 (MQ=255)
              acgacCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAgg                                                                                                                                   <  1:620814/150‑1 (MQ=255)
                        cACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGTTGTGCCAATGATCGATTCTATCTCTGCAGAGCCGTGCGCGTGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTaaaa                                                                                                                         <  1:248256/150‑1 (MQ=255)
                        cACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTATGGCAGTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTaaaa                                                                                                                         <  2:666737/150‑1 (MQ=255)
                         acgacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGaa                                                                                                                                                 >  2:729894/1‑125 (MQ=255)
                         acgacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGaa                                                                                                                                                 <  1:729894/125‑1 (MQ=255)
                                 tGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACt                                                                                                                >  1:121402/1‑150 (MQ=255)
                                    ttGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                                      >  2:758146/1‑141 (MQ=255)
                                    ttGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                                      <  1:758146/141‑1 (MQ=255)
                                          ggCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAagag                                                                                                                                              <  2:888448/111‑1 (MQ=255)
                                          ggCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAagag                                                                                                                                              >  1:888448/1‑111 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAgg                                                                                                                                             <  2:630098/104‑1 (MQ=255)
                                                  ccccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAgg                                                                                                                                             >  1:630098/1‑104 (MQ=255)
                                                     cAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            <  1:713772/150‑1 (MQ=255)
                                                     cAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                                            >  1:690689/1‑150 (MQ=255)
                                                      aaGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGACACTGTAGCTACCCGTCACCACAAg                                                                                           >  1:45441/1‑150 (MQ=255)
                                                             gATCAGCGAAGCAGAGATTGAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACCGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                                    <  1:297495/150‑1 (MQ=255)
                                                             gATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                                    <  2:33501/150‑1 (MQ=255)
                                                                     aaGCAGAGATGCAGGCGCTGATGGCGGTGTGGCAAGGGTCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGcc                                                                            >  1:441453/1‑150 (MQ=255)
                                                                         agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCAcc                                                                        >  2:269414/1‑150 (MQ=255)
                                                                            gaTGCAGGCGCTGATGGCGCTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTgg                                                                     <  1:379239/150‑1 (MQ=255)
                                                                                                                tGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTc                                 <  2:121402/150‑1 (MQ=255)
                                                                                                                 gCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCt                                >  2:596781/1‑150 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGtagtag                                    <  1:896627/136‑1 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGtagtag                                    >  2:896627/1‑136 (MQ=255)
                                                                                                                           cTGCCTCTTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt                      >  2:785505/1‑150 (MQ=255)
                                                                                                                             gCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGt                    >  1:705016/1‑150 (MQ=255)
                                                                                                                               ctctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt                      <  2:700045/146‑1 (MQ=255)
                                                                                                                               ctctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt                      >  1:700045/1‑146 (MQ=255)
                                                                                                                                tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGt                             >  1:722898/1‑138 (MQ=255)
                                                                                                                                tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGt                             <  2:722898/138‑1 (MQ=255)
                                                                                                                                tctTCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGc                 >  2:193443/1‑150 (MQ=255)
                                                                                                                                  ttCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTc               >  1:115692/1‑150 (MQ=255)
                                                                                                                                     gcgagaaTGCTCCGGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAAt            <  2:115692/144‑1 (MQ=255)
                                                                                                                                            tGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGccgccc     >  2:95614/1‑150 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                               <  2:641500/57‑1 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                               >  1:641500/1‑57 (MQ=255)
                                                                                                                                               tCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCCCCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGca  >  2:449085/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
GCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCTCAATGCCGCCCGCA  >  NC_002947/321999‑322291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑