Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,282,8220CA42.4% 23.9 / 30.4 33intergenic (‑171/+58)PP_2010/PP_2011cytochrome b561/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (10/9);  new base A (6/8);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC  >  NC_002947/2282675‑2282968
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gAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCt                                                                                                                                                   <  2:544987/150‑1 (MQ=255)
     gcgcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCGACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCa                                                                                                                                              <  1:73115/150‑1 (MQ=255)
     gcactctGGCTGTCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCa                                                                                                                                              <  2:141248/147‑1 (MQ=37)
       gcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCTACGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGACCAACTGCACAGGCTTCCAAGACTGTGCAGt                                                                                                                                            <  1:602940/150‑1 (MQ=37)
       gcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCCCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGt                                                                                                                                            <  1:356600/150‑1 (MQ=37)
        ctctTTCTGTCGCTCTGGAGTCAGTTTAATCGGTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTTCAAGACTGTGCAGtt                                                                                                                                           <  2:125625/150‑1 (MQ=25)
        ctctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCGGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt                                                                                                                                           <  2:209747/150‑1 (MQ=25)
                ggCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACtgtg                                                                                                                                   <  1:888873/150‑1 (MQ=25)
                              aTTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgtg                                                                                                                                   <  1:305557/136‑1 (MQ=255)
                              aTTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgtg                                                                                                                                   >  2:305557/1‑136 (MQ=255)
                                            cGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtgt                                                                                                                                                 >  2:195322/1‑108 (MQ=255)
                                            cGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtgt                                                                                                                                                 <  1:195322/108‑1 (MQ=255)
                                                        tttGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt                                                                                                                                           >  1:476863/1‑102 (MQ=18)
                                                        tttGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt                                                                                                                                           <  2:476863/102‑1 (MQ=18)
                                                                                                  tctTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAac                                                >  2:125564/1‑150 (MQ=255)
                                                                                                           gCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCt                                       >  1:521926/1‑150 (MQ=255)
                                                                                                                    cTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGc                              <  1:125564/150‑1 (MQ=255)
                                                                                                                       ccACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAg                           >  1:452589/1‑150 (MQ=25)
                                                                                                                        cacaGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAgg                          >  1:156923/1‑150 (MQ=255)
                                                                                                                         acaGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGc                         <  2:156923/150‑1 (MQ=255)
                                                                                                                             tCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCa                     >  2:46133/1‑150 (MQ=255)
                                                                                                                                aaCTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCg                                                                                                        >  1:574499/1‑65 (MQ=255)
                                                                                                                                aaCTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCg                                                                                                        <  2:574499/65‑1 (MQ=255)
                                                                                                                                aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAg                           <  2:102330/141‑1 (MQ=37)
                                                                                                                                aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAg                           >  1:102330/1‑141 (MQ=37)
                                                                                                                                aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGTGGCTGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATc                   >  1:783718/1‑150 (MQ=18)
                                                                                                                                   tGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCa                                                                   >  1:296112/1‑98 (MQ=255)
                                                                                                                                   tGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCa                                                                   <  2:296112/98‑1 (MQ=255)
                                                                                                                                   tGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCATCATCGGCa               >  2:453835/1‑150 (MQ=255)
                                                                                                                                   tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCTCCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa               >  2:846865/1‑150 (MQ=37)
                                                                                                                                     cacaGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGt                                                                 <  2:245134/98‑1 (MQ=255)
                                                                                                                                     cacaGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGt                                                                 >  1:245134/1‑98 (MQ=255)
                                                                                                                                                aaGACTGTGCAGTTGGACTGGGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTACCTCCTGCCAGGCACCATCGGCATCAGGCACCATTc  >  1:867023/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCCGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGG‑AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC  >  NC_002947/2282675‑2282968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑