| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,295,019 | Δ1 bp | 17.9% | intergenic (+29/+41) | slyB → / ← nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,295,019 | 0 | A | . | 17.9% | 73.9 / 12.8 | 28 | intergenic (+29/+41) | slyB/nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base A (13/10); new base . (3/2); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
GATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCG > NC_002947/1294880‑1295159 | gatgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc > 2:502442/1‑150 (MQ=255) tgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCa > 2:204477/1‑150 (MQ=255) caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGAATGTAGACGGTACCAGTCGCGTTTCGCACTGACAGTAGAAAAAAAAAAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg < 2:21915/150‑1 (MQ=255) gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGtttt < 1:502442/150‑1 (MQ=255) aTGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATg < 2:517399/150‑1 (MQ=255) ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc < 1:210772/115‑1 (MQ=255) ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc > 2:210772/1‑115 (MQ=255) gAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCt > 1:477833/1‑150 (MQ=255) ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt > 2:458672/1‑150 (MQ=255) gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc > 1:374222/1‑135 (MQ=255) gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc < 2:374222/135‑1 (MQ=255) tGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa < 1:458672/150‑1 (MQ=255) gACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAc > 1:158833/1‑150 (MQ=255) aCGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAcc > 2:485287/1‑150 (MQ=255) tACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGGTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCgcag > 1:43698/1‑150 (MQ=255) cACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGgctg > 1:597169/1‑41 (MQ=255) cACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGgctg < 2:597169/41‑1 (MQ=255) cACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGt < 1:204477/150‑1 (MQ=255) aCTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTa > 2:338931/1‑150 (MQ=255) cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt < 1:573036/90‑1 (MQ=255) cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt > 2:573036/1‑90 (MQ=255) aGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCg > 2:298593/1‑150 (MQ=255) taataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGt < 1:338931/150‑1 (MQ=255) atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa < 2:194916/120‑1 (MQ=37) atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa > 1:194916/4‑123 (MQ=37) aaaaaaCCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCAt < 2:158833/150‑1 (MQ=255) aaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGcc > 1:394478/1‑150 (MQ=39) aaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACACAAGAAAACAACGACCCACGAAGAATTCCCATGCGGGCCATGCCg > 2:440178/1‑150 (MQ=255) | GATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCG > NC_002947/1294880‑1295159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |