| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,907,968 | Δ1 bp | 13.3% | coding (55/1095 nt) | spuE ← | spermidine/putrescine ABC transporter substrate‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,907,968 | 0 | C | . | 13.3% | 81.9 / 9.3 | 30 | coding (55/1095 nt) | spuE | spermidine/putrescine ABC transporter substrate‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (14/11); new base . (2/2); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.59e-01 | |||||||||||
TTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGAT > NC_002947/5907828‑5908109 | ttCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAg > 2:114854/1‑150 (MQ=255) ccAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGc < 1:114854/150‑1 (MQ=255) ggTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCcgc < 2:279174/150‑1 (MQ=255) tCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTACGCGAGTGTGGTGGGGCATATGTAGTCGTACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCgcca > 1:383016/1‑150 (MQ=255) gAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTccaagcg > 2:432348/1‑127 (MQ=255) gAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCTGGCATG‑CAGCGTccaagcg < 1:432348/133‑7 (MQ=255) gAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGc > 1:405385/1‑150 (MQ=255) gAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGc > 2:480394/1‑150 (MQ=255) gacacATGTTGCACAGGCATGATCCCCGTGGCTTTCTTCAAGGCGGCGAGAGTGGTGGGGCCGGTGGAGCCGGAACAGTTCTAGAAGTGCACCGTCGGCGCCGCTTGGACGCG‑GCATGGCAGCGTCAAGCCCGCGCCAGCCCTCaatggc > 2:182626/2‑146 (MQ=255) acaTCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTg > 1:439318/1‑150 (MQ=255) ttGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATa < 2:383016/150‑1 (MQ=255) ttttCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGt < 2:290016/149‑1 (MQ=255) tttCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTc > 2:507883/1‑150 (MQ=255) aaGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACaa > 2:432113/1‑150 (MQ=255) aGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAAt < 1:410800/150‑1 (MQ=255) gtgtGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTg < 2:405385/150‑1 (MQ=255) tggtggGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGt > 1:311924/1‑150 (MQ=255) gCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTCGGTAGCGaga > 2:477993/1‑148 (MQ=255) cGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAt > 1:240152/1‑150 (MQ=255) cGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAt < 1:182626/150‑1 (MQ=255) cGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAt < 2:311924/150‑1 (MQ=255) tGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAAtgct < 1:532199/150‑1 (MQ=255) aGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGt < 1:145722/112‑1 (MQ=255) aGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGt > 2:145722/1‑112 (MQ=255) ctgACGCTGGCATG‑CAGCGTCCGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGc < 2:42006/98‑1 (MQ=255) ctgACGCTGGCATG‑CAGCGTCCGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGc > 1:42006/3‑100 (MQ=255) gCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACGGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGAt > 1:478010/1‑150 (MQ=255) gCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGAt > 2:520205/1‑150 (MQ=255) aTGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCaact < 2:268143/127‑1 (MQ=255) aTGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCaact > 1:268143/1‑127 (MQ=255) | TTCCAGGGTTTCGTTGGAGTCGAACACATCTTGCACAGGCTTGATGCCCGTGGCTTTCTCGAAGTCCGCGAGTGTGGTGGGGCCGATGTAGTCGGACCAGTTATAGAAGTGCACCGTCGGCGCCGCTTGGACGCT‑GCATGCCAGCGTCAGGCCCGCGCCAGCCATCAAGGCCTTGCGGAATACAGAAATAGACAAGTGGGGGTCCTCACAATCAGTGCCTGGGTAGCGACAATGCTGCCGCACACGCAAAACCGGCGCGCAACTTAACTTCGCAGCTTCGAT > NC_002947/5907828‑5908109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |