| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,344,114 | G→C | 14.8% | A10G (GCC→GGC) ‡ | PP_1168 ← | TRAP dicarboxylate transporter subunit DctQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,344,114 | 0 | G | C | 14.8% | 69.6 / 6.3 | 27 | A10G (GCC→GGC) ‡ | PP_1168 | TRAP dicarboxylate transporter subunit DctQ |
| Reads supporting (aligned to +/- strand): ref base G (11/12); new base C (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01 | |||||||||||
CCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGT > NC_002947/1343986‑1344253 | cccACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAt > 1:312944/1‑150 (MQ=255) cATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTa < 1:55844/150‑1 (MQ=255) ccAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa < 2:424449/150‑1 (MQ=255) aGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACg > 1:451477/1‑150 (MQ=255) tCCAGATGCAACGGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatca < 2:34553/150‑1 (MQ=255) cAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAgg < 1:352602/150‑1 (MQ=255) aGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatca < 2:552472/147‑1 (MQ=255) aGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatca > 1:552472/1‑147 (MQ=255) gTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGt < 2:529372/73‑1 (MQ=255) gTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGt > 1:529372/1‑73 (MQ=255) gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatca < 1:341523/110‑1 (MQ=255) gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcatca > 2:341523/1‑110 (MQ=255) gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat < 2:218459/108‑1 (MQ=255) gAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTtcat > 1:218459/1‑108 (MQ=255) aTTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTg > 1:390854/1‑79 (MQ=255) aTTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTg < 2:390854/79‑1 (MQ=255) tCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtcgt > 2:275285/1‑150 (MQ=255) cATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGAGTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTa < 1:67341/150‑1 (MQ=255) ggCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAAc > 1:400678/1‑150 (MQ=255) aGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTc < 2:312944/150‑1 (MQ=255) cGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGa < 2:400678/150‑1 (MQ=255) ggCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa < 1:457759/59‑1 (MQ=18) ggCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGa > 2:457759/1‑59 (MQ=18) gCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc > 1:245263/1‑128 (MQ=255) gCAGTGCGGCGCCCGCCGCCCTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGcgtc < 2:245263/128‑1 (MQ=255) aGGGCGGCGGGCGCCTCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGt > 1:185741/1‑150 (MQ=255) aGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGt > 1:513488/1‑150 (MQ=255) | CCCACGGGATGATCAGCGTCATGGCCAGGATCGACAGGCCCGCGATCCAGATGCAACTGCGGTACAGCGTGTCGTTCACGCTCAGGAAAAGCGATTTCATAGGCATCACCAAAGCGGCAGGGCGGCGGGCGCCGCACTGCCGAGGTCGATTGGGCAAGGGCGGCTGTTACTGGACGGCGTCGATACGCTTCATCAGGTCGGCGTACTGCGCGCCGTATTTTTCGCGTACCGAAGCCGTGGCGTCGTAGAACGGCTTCTTGTCGACGGT > NC_002947/1343986‑1344253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |