| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,819,838 | T→C | 16.7% | intergenic (+33/‑442) | rpoS → / → PP_1624 | RNA polymerase sigma S factor/group II intron‑encoding maturase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,819,838 | 0 | T | C | 16.7% | 51.3 / 4.1 | 24 | intergenic (+33/‑442) | rpoS/PP_1624 | RNA polymerase sigma S factor/group II intron‑encoding maturase |
| Reads supporting (aligned to +/- strand): ref base T (12/8); new base C (2/2); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCGCGGGTG > NC_002947/1819697‑1819987 | gaTCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCgg > 1:223565/1‑149 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCACCGGGGCGtttttt < 1:451781/145‑2 (MQ=255) aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCACCGGGGCGtttttt > 2:451781/1‑144 (MQ=255) tCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCt > 1:408004/1‑150 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg < 1:470776/137‑1 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg > 2:470776/1‑137 (MQ=255) ttGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTggg > 2:333010/1‑150 (MQ=255) aaTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCACCGGGGCGttttttg < 2:493518/80‑3 (MQ=21) aaTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCACCGGGGCGttttttg > 1:493518/1‑78 (MQ=21) gTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTa < 2:77080/100‑1 (MQ=255) gTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTa > 1:77080/1‑100 (MQ=255) cAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTc > 1:518225/1‑150 (MQ=255) aGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTcc > 2:528281/1‑150 (MQ=255) cGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc < 1:120438/150‑1 (MQ=255) aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacaca > 2:359606/1‑150 (MQ=255) ttCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGCTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCg > 2:218217/1‑150 (MQ=255) ctagcGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGa > 1:494547/1‑150 (MQ=255) tGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTa < 2:408004/150‑1 (MQ=255) aaaaCGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACGTGGAGGTGAATGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAgg < 2:330067/150‑1 (MQ=255) aaaCGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt > 2:120299/1‑137 (MQ=255) aaaCGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt < 1:120299/137‑1 (MQ=255) cccGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGgt > 1:141001/1‑150 (MQ=255) aTC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCGCgg < 1:333010/150‑1 (MQ=35) aTC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCGCgg < 2:494547/150‑1 (MQ=38) tcGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCGCGGGTg < 2:518225/150‑1 (MQ=35) | GATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGAATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑TCGGGGCGTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCGCGGGTG > NC_002947/1819697‑1819987 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |