| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,851,912 | A→G | 100% | D319G (GAC→GGC) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,851,912 | 0 | A | G | 97.2% | 88.1 / ‑5.9 | 29 | D319G (GAC→GGC) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (16/12); total (17/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGC > NC_002947/1851771‑1852057 | gcAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACa > 2:519592/1‑150 (MQ=255) aGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCCCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTACAGATCGTCTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCCAcc < 1:573801/150‑1 (MQ=255) gcgcAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAAccc > 2:416848/1‑150 (MQ=255) gcAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTACGGTGCGCGTGCTGGTGCCGGAAATCCGGGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAAccccc > 2:356539/1‑149 (MQ=255) aaaGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAg > 2:57069/1‑150 (MQ=255) tGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACg > 2:500317/1‑150 (MQ=255) tGGACTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACg > 2:500322/1‑150 (MQ=255) ggAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCggcgac < 1:207141/124‑1 (MQ=255) ggAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCggcgac > 2:207141/1‑124 (MQ=255) gTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTa < 1:331711/150‑1 (MQ=255) cAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCg < 1:519592/150‑1 (MQ=255) aGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGc > 2:115039/1‑150 (MQ=255) ccAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTc < 1:122340/150‑1 (MQ=255) gATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCCCCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGtt < 1:500322/150‑1 (MQ=255) gATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCAAAGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGtt < 1:500317/150‑1 (MQ=255) aTCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCTCCAACCCCAAg < 2:183111/129‑1 (MQ=255) aTCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCCACCCCAAGGAGAACGGCTACCGCTCGTTg < 1:420837/150‑1 (MQ=255) aTCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAg > 1:183111/1‑129 (MQ=255) tACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAg > 2:198948/1‑126 (MQ=255) tACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAg < 1:198948/126‑1 (MQ=255) tgcgtgcgGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCgg > 1:254313/1‑150 (MQ=255) gTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAg < 1:115039/150‑1 (MQ=255) gcgACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCt < 1:184938/96‑1 (MQ=255) gcgACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCt > 2:184938/1‑96 (MQ=255) tACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCgg < 2:91498/116‑1 (MQ=255) tACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCgg > 1:91498/1‑116 (MQ=255) cTTGTGGCGGCATATTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCACGGTGCACACTCCGGCAACCCGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGa > 1:44096/1‑150 (MQ=255) tatTCCCAAGGAGTTCGGCGACTACATCGCCAACCCCGAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATg > 2:215475/1‑150 (MQ=255) ttCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcg > 1:52038/1‑150 (MQ=255) ccAAGGAGTTCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGGGTATgggcgc > 2:141931/1‑148 (MQ=255) ttCGGCGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGAGCAAGGTGGTGGGGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc > 1:382603/1‑150 (MQ=255) | GCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGC > NC_002947/1851771‑1852057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |