Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,253,967 G→A 14.3% K81K (AAG→AAA leuB → 3‑isopropylmalate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,253,9670GA14.3% 62.2 / 3.6 28K81K (AAG→AAAleuB3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (12/12);  new base A (2/2);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

CTCGGCTTCAGCCTGGCGCACGACGTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTC  >  NC_002947/2253824‑2254101
                                                                                                                                               |                                                                                                                                        
atCGGCTTCAGCCTGGCGCACGACGTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATcgag                                                                                                                                    >  2:289880/2‑150 (MQ=255)
    gCTTCAGCCTGGCGCACGACGTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATcgagcgag                                                                                                                                <  2:465897/150‑1 (MQ=255)
                 gcACGACGTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGa                                                                                                                   >  2:335245/1‑150 (MQ=255)
                     gacgTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAgcgc                                                                                                               >  1:406370/1‑150 (MQ=255)
                               gCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATcga                                                                                                                                     >  1:193065/1‑118 (MQ=255)
                               gCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATcga                                                                                                                                     <  2:193065/118‑1 (MQ=255)
                                 ggTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAg                                                                                                   >  1:214299/1‑150 (MQ=255)
                                     ccgccATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATcc                                                                                               <  1:335245/150‑1 (MQ=255)
                                                    aCGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGcc                                                                               <  2:395715/150‑1 (MQ=255)
                                                                tGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGaa                                                                                                    <  1:112125/118‑1 (MQ=255)
                                                                tGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGaa                                                                                                    >  2:112125/1‑118 (MQ=255)
                                                                tGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAAcc                                                                  <  2:222930/150‑1 (MQ=255)
                                                                             ccTGGAGCGTGCGCGCAACGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGAACGAGCGAGACATTCGCCCGGAGCGCCGCCTGCTGAAGATCTGCTCGCAACTGGGCCTGTTCTCCAACCTGCGCCCGGCCAt                                                     >  2:354292/1‑150 (MQ=255)
                                                                              cTGGAGCGTGCGCGCAAGGTCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATc                                                    <  2:406370/150‑1 (MQ=255)
                                                                                         gcgcAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATc                                                                                                >  1:413316/1‑97 (MQ=255)
                                                                                         gcgcAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATc                                                                                                <  2:413316/97‑1 (MQ=255)
                                                                                             aaGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACta                                     >  1:93883/1‑149 (MQ=255)
                                                                                                  cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCCGccctt                                                    >  1:285601/1‑127 (MQ=255)
                                                                                                  cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCCGccctt                                                    <  2:285464/130‑4 (MQ=255)
                                                                                                  cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCCGccctt                                                    <  2:285601/130‑4 (MQ=255)
                                                                                                  cGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAAATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCCGccctt                                                    >  1:285464/1‑127 (MQ=255)
                                                                                                                tgGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGa                  >  1:519036/1‑150 (MQ=255)
                                                                                                                   gggCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGc               <  2:214299/150‑1 (MQ=255)
                                                                                                                      cTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCgg            <  2:551531/150‑1 (MQ=255)
                                                                                                                          gggcgggcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGCGCCTGTTCGCCAACGCCGCCCCGGCCAGCCGTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAAt        <  1:537723/150‑1 (MQ=255)
                                                                                                                                gcCGAAGTGGGACCCGATCGAGCTAAACATTCTCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGAATTGCGTCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc  <  1:289880/150‑1 (MQ=255)
                                                                                                                                gcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc  >  1:535097/1‑150 (MQ=255)
                                                                                                                                gcCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTc  >  2:607588/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                        
CTCGGCTTCAGCCTGGCGCACGACGTGATTGGCGGTGCCGCCATCGACAAGCACGGTGTGCCGCTGGCCGACGAAACCCTGGAGCGTGCGCGCAAGGCCGATGCAGTGCTGCTGGGGGCTGTGGGCGGGCCGAAGTGGGACAAGATCGAGCGAGACATTCGCCCGGAGCGCGGCCTGCTGAAGATCCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGCCCGGCCATCCTTTACCCGCAACTGGCCGATGCCTCGTCGCTGAAGCCGGAAATCGTGTC  >  NC_002947/2253824‑2254101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: