| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,288,420 | C→A | 11.8% | Y220* (TAC→TAA) | pepN → | aminopeptidase N |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,288,420 | 0 | C | A | 11.8% | 94.3 / 4.3 | 34 | Y220* (TAC→TAA) | pepN | aminopeptidase N |
| Reads supporting (aligned to +/- strand): ref base C (14/16); new base A (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
CCGGCCGAAGATGGCCGCCACTGGGCGACCTGGGAAGACCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCTTCATGATTGTTGCGGTCAATGACTTCAACATGGGC > NC_002947/2288274‑2288561 | ccggccgAAGATGGCCGCCACTGGGCGACCTGGGAAGGCCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTc < 2:510970/150‑1 (MQ=255) gccgAAGATGGCCGCCACTGGGCGACCTGGGAAGACCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTTAGGTGACCCTGCGCATCTACGTCGaa < 1:93660/150‑1 (MQ=255) gccACTGGGCGACCTGTGAAGACCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTTCGCATCTACGTCGAACCCGAGAACATCg > 1:453468/1‑150 (MQ=255) tGGGAAGACCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCAc > 2:337179/1‑150 (MQ=255) cGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCa < 2:382021/150‑1 (MQ=255) gTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAg < 1:337179/150‑1 (MQ=255) caagAAGCTGGCCTACCTGTCCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCt < 2:557032/147‑1 (MQ=255) aTGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTc > 2:56622/1‑105 (MQ=255) aTGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTc < 1:56622/105‑1 (MQ=255) cGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGaa > 1:199400/1‑109 (MQ=255) cGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGaa < 2:199400/109‑1 (MQ=255) gCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCAc < 1:54282/126‑1 (MQ=255) gCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCAc > 2:54282/1‑126 (MQ=255) gCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCAAGTCCGACCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTc > 1:296148/1‑150 (MQ=255) cGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACgaa > 1:342613/1‑142 (MQ=255) cGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACgaa < 2:342613/142‑1 (MQ=255) tgtgGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGc < 1:528330/97‑1 (MQ=255) tgtgGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGc > 2:528330/1‑97 (MQ=255) cGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCAt > 1:359433/1‑93 (MQ=255) cGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCAt < 2:359433/93‑1 (MQ=255) gACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGAc > 2:185074/1‑150 (MQ=255) aCAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATg < 1:327574/129‑1 (MQ=255) aCAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATg > 2:327574/1‑129 (MQ=255) aCAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTg < 2:23046/146‑1 (MQ=255) aCAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTg > 1:23046/1‑146 (MQ=255) aGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTAAGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCATCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTAc > 2:304334/1‑138 (MQ=255) aGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTAAGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCATCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTAc > 2:304030/1‑138 (MQ=255) aGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTAAGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCATCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTAc < 1:304334/138‑1 (MQ=255) aGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTAAGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCATCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTAc < 1:304030/138‑1 (MQ=255) cTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCtt < 1:185074/150‑1 (MQ=255) ccGCAAGTCAGGCAGTGAGGTGACCATGAGCATCTACGTCGAACCCGAGAAAATCGAAAAGTGCGACCACGCCATAGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTATATGACAGCGAGTACAACCTGGACATCTTCATGAt > 2:433931/1‑150 (MQ=255) cAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCTTCATGAttgtt < 2:590986/150‑1 (MQ=255) ggTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCTTCATGATTGTTGCGGTCAATGACtt > 2:474681/1‑150 (MQ=255) cgcATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACTAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCTTCATGATTGTTGCGGTCAATGACTTCAACATGGgc < 2:453468/150‑1 (MQ=255) | CCGGCCGAAGATGGCCGCCACTGGGCGACCTGGGAAGACCCGTTCATGAAGCCGGCCTACCTGTTCGCCCTGGTGGCCGGTGACCTGTGGTGCGTTGAAGACAGCTTCACCCGCCAGTCCGGCCGTGAGGTGACCCTGCGCATCTACGTCGAACCCGAGAACATCGACAAGTGCGACCACGCCATGGTCAGCCTGAAAAAGTCCATGCGCTGGGACGAAGAAGTCTATGGCCGCGAGTACGACCTGGACATCTTCATGATTGTTGCGGTCAATGACTTCAACATGGGC > NC_002947/2288274‑2288561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |