Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,568,747 T→A 17.1% M282L (ATG→TTG)  PP_2252 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,568,7470TA17.1% 100.4 / 14.4 41M282L (ATG→TTG) PP_2252hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (18/16);  new base A (2/5);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

GCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGC  >  NC_002947/2568600‑2568891
                                                                                                                                                   |                                                                                                                                                
gCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCt                                                                                                                                                >  2:74461/1‑150 (MQ=255)
  cgcgGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACGCGCAGTTGTTCGACCATCTGc                                                                                                                                              >  1:364255/1‑150 (MQ=255)
    cggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            <  2:499128/150‑1 (MQ=255)
    cggcgCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGACGGTCGAACAACTGCgg                                                                                                                                            <  2:453168/150‑1 (MQ=255)
        gcAGTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGggtgg                                                                                                                                        <  2:100895/150‑1 (MQ=255)
           cTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCGGGTGATcc                                                                                                                                     <  1:439986/150‑2 (MQ=255)
              gCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACgg                                                                                                                                  >  2:170271/1‑150 (MQ=255)
              gCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACgg                                                                                                                                  <  1:206056/150‑1 (MQ=255)
                cAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTc                                                                                                                                <  1:523640/150‑1 (MQ=255)
                       cTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtc                                                                                                                         >  1:362144/1‑150 (MQ=255)
                         gTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGtcat                                                                                                                       <  2:280815/150‑1 (MQ=255)
                            cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            <  1:62514/126‑1 (MQ=255)
                            cTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCgg                                                                                                                                            >  2:62514/1‑126 (MQ=255)
                                               tAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCa                                                                                                 >  2:292057/1‑150 (MQ=255)
                                                  tAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATc                                                                                              <  2:362144/150‑1 (MQ=255)
                                                        cccGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAatc                                                                                        >  2:595232/1‑150 (MQ=255)
                                                                  ccccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTg                                                                              <  1:492916/150‑1 (MQ=255)
                                                                    ccGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGcc                                                                            >  1:152173/1‑150 (MQ=255)
                                                                      gCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                            <  1:260694/100‑1 (MQ=255)
                                                                      gCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATgg                                                                                                                            >  2:260694/1‑100 (MQ=255)
                                                                           ggCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGc                                                                     <  1:161425/150‑1 (MQ=255)
                                                                             cGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCgg                                                                                                                                            >  1:144913/1‑77 (MQ=255)
                                                                             cGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCgg                                                                                                                                            <  2:144913/77‑1 (MQ=255)
                                                                                      aTCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCACTGCCACCTTGCCCACTGCCGAt                                                          >  1:493888/1‑150 (MQ=255)
                                                                                         aaCTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGcc                                                       <  1:190208/150‑1 (MQ=255)
                                                                                         aaCTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGcc                                                       <  1:190237/150‑1 (MQ=255)
                                                                                                gCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCAc                                                >  2:402266/1‑150 (MQ=255)
                                                                                                    ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGc                                            >  2:36242/1‑150 (MQ=255)
                                                                                                    ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCggg                                                                                                                                           >  1:151555/1‑55 (MQ=17)
                                                                                                    ttCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGATGGTCGAACAACTGCggg                                                                                                                                           <  2:151555/55‑1 (MQ=17)
                                                                                                      ccTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGa                                          <  2:552282/150‑1 (MQ=255)
                                                                                                          gTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATccc                                      >  1:170462/1‑150 (MQ=255)
                                                                                                            tCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCcgc                                    <  2:493888/150‑1 (MQ=255)
                                                                                                                cGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTgg                                >  1:2617/1‑150 (MQ=255)
                                                                                                                 gCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTggg                               <  1:74461/150‑1 (MQ=255)
                                                                                                                      gTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGTTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAg                          >  1:594945/1‑150 (MQ=255)
                                                                                                                        tCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAggg                        >  1:190585/1‑150 (MQ=255)
                                                                                                                                        ttgttCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTc        <  1:170271/150‑1 (MQ=255)
                                                                                                                                          gttCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTgg                                             <  2:158393/111‑1 (MQ=255)
                                                                                                                                          gttCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTgg                                             >  1:158393/1‑111 (MQ=255)
                                                                                                                                              gACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGc  >  2:464864/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GCCGCGGCGCACTGGCCAGCAAGCTGTCCTCAACCTGCCCCGACAGGTAATAGACCCCCGCCATGGCCCCGCTCTGGCGGTTCTCCATCAACTCCTGCCATTCCTGGTTCAACGCCACGTTCAGGATCACCCGCAGTTGTTCGACCATCTGCGGGTGGTCACGGTCATGGGTCATCACCCCGTAACCGGCTGCGGCAATCGAAATCATCGCCCCTGCCACCTTGCCCACTGCCGATGCCACGGCACTGGCGATCCCGCGTGGGGCCAGGGTGGCGCCAAGCTTTTCGCTGGC  >  NC_002947/2568600‑2568891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑