Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,183,925 G→A 12.8% V124M (GTG→ATG) ‡ PP_2794 → short‑chain dehydrogenase/reductase family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,183,9250GA12.8% 77.4 / 2.1 31V124M (GTG→ATG) ‡PP_2794short‑chain dehydrogenase/reductase family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (15/12);  new base A (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGCCCACCTGACC  >  NC_002947/3183780‑3184065
                                                                                                                                                 |                                                                                                                                            
ggACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGc                                                                                                                                          >  2:533574/1‑150 (MQ=255)
                               ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAggcgc                                                                                                                                  <  2:532521/127‑4 (MQ=255)
                               ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAggcgc                                                                                                                                  >  1:532521/1‑124 (MQ=255)
                               ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAgg                                                                                                                                     <  1:405719/124‑1 (MQ=255)
                               ccGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCACGGCCCAgg                                                                                                                                     >  2:405719/1‑124 (MQ=255)
                                cGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCgg                                                                                                          <  1:573865/150‑1 (MQ=255)
                                cGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTCCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCgg                                                                                                          <  1:539088/150‑1 (MQ=255)
                                   tGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTACGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGa                                                                                                       >  2:415310/1‑150 (MQ=255)
                                        gTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg                                                                                                  <  2:261270/150‑1 (MQ=255)
                                         ttCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAggggg                                                                                                 >  1:295464/1‑150 (MQ=255)
                                              gTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGc                                                                                            >  1:409699/1‑150 (MQ=255)
                                                 aacaaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTg                                                                                         >  2:417571/1‑150 (MQ=255)
                                                  acaaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGa                                                                                        >  2:32931/1‑150 (MQ=255)
                                                   caaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGAt                                                                                       <  1:32931/150‑1 (MQ=255)
                                                    aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCAtggtggt                                                                                                               >  1:521852/1‑125 (MQ=255)
                                                    aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCAtggtggt                                                                                                               <  2:521852/125‑1 (MQ=255)
                                                    aaTGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATc                                                                                      <  1:533574/150‑1 (MQ=255)
                                                      tGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCaa                                                                                    <  2:295464/150‑1 (MQ=255)
                                                      tGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTACGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCaa                                                                                    <  1:415310/150‑1 (MQ=255)
                                                       gCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAAt                                                                                   <  2:409699/150‑1 (MQ=255)
                                                                    aCAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATcc                                                                      >  2:169265/1‑150 (MQ=255)
                                                                        ccagccTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGCGGACCTGCAAGGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGc                                                                  >  1:500562/1‑150 (MQ=255)
                                                                             cTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg                                                                                                  >  2:537147/1‑113 (MQ=255)
                                                                             cTTTGCTAGCCTGCGATGATCAAACCTGGGACCAAGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAgggg                                                                                                  <  1:537147/113‑1 (MQ=255)
                                                                                  cTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCGGGGCCGTGGCCCAGGAAAGCACCCGGCGGATGGTGGTGGCGTTGAATTTGCCGGGGCTTATCAATGTCACCTCGATCCTCGCCAGCCGtgtg                                                        <  1:169265/150‑1 (MQ=255)
                                                                                                             cACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTggcg                             >  2:83241/1‑150 (MQ=255)
                                                                                                             cACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTggcg                             <  1:343754/150‑1 (MQ=255)
                                                                                                                          ccAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAggccggcc                >  2:565727/1‑150 (MQ=255)
                                                                                                                            aaCCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTg              >  1:367747/1‑150 (MQ=255)
                                                                                                                                tCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGccc          >  1:224218/1‑150 (MQ=255)
                                                                                                                                        gcCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCAGCCAAGGCCGGCCTGGCCCACCTAAcc  >  1:282067/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
GGACATCTGCCGGGTGCTCGATGCCGCCGGCCCGCTGGATGTTCTGGTCAACAATGCGGGGGTGAGCGACAGCCAGCCTTTGCTAGCCTGCGATGATCAAACCTGGGACCACGTGCTCGACACCAACCTCAAGGGCGCCTGGGCCGTGGCCCAGGAAAGCGCCCGGCGCATGGTGGTGGCGGGGAAGGGGGGCAGCCTGATCAATGTCACCTCGATCCTCGCCAGCCGTGTGGCCGGCGCCGTCGGCCCTTACCTGGCGGCCAAGGCCGGCCTGGCCCACCTGACC  >  NC_002947/3183780‑3184065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑