| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,313,871 | T→G | 19.4% | intergenic (+33/‑126) | hemB → / → proP | 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,313,871 | 0 | T | G | 19.4% | 46.1 / 3.6 | 24 | intergenic (+33/‑126) | hemB/proP | 5‑aminolevulinate dehydratase/osmosensory proline/betaine/H+ permease |
| Reads supporting (aligned to +/- strand): ref base T (7/12); new base G (2/3); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTC > NC_002947/3313730‑3314006 | cATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGc < 1:520480/150‑1 (MQ=255) aTCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCt > 2:361296/1‑150 (MQ=255) gcgAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGggtg < 1:361296/150‑1 (MQ=255) ggCGCAATCAAGCGTGCTGGTGCAGCCAGGAGCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAAc < 1:309501/150‑1 (MQ=255) gcgcAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACg > 2:540415/1‑150 (MQ=255) gCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGAGCCGTTGGGTGGTGCCGCAAACGCACaaaaaaa < 2:587203/150‑1 (MQ=255) ccTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAat > 2:577049/1‑150 (MQ=255) cTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTcc < 1:540415/150‑1 (MQ=255) gCCATGGCCCTGGCGCTCTAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGACGCGGCGGTGATCGCGGTGGTGCTTTATGCGCCGTTGGGTGGTGCCGCACACGCTCAATAAAACAGTATGCGCTGAATTATTTAGTGTCCTGTTGTa < 1:294391/150‑1 (MQ=255) ccATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTAt > 2:519619/1‑150 (MQ=255) ccTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATcc < 1:207776/150‑1 (MQ=255) tGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCt < 1:577049/150‑1 (MQ=255) gcgcgAAGGTATCTGAAGCCAGGCCTTGGTAATGAGAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTaga < 1:526178/150‑1 (MQ=255) gCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGa < 1:578015/150‑1 (MQ=255) gCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGa > 2:578015/1‑150 (MQ=255) tGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTa > 2:40207/1‑104 (MQ=255) tGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTa < 1:40207/104‑1 (MQ=255) aaaGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTc < 2:576645/150‑1 (MQ=255) aaaGCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTAt > 2:4745/1‑142 (MQ=255) aaaGCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTAt < 1:4745/142‑1 (MQ=255) aGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAat > 2:200965/1‑81 (MQ=255) aGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAat < 1:200965/81‑1 (MQ=255) gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGAc < 2:21036/125‑1 (MQ=255) gCCGCTGCAATGGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGAc > 1:21036/1‑125 (MQ=255) | CATCGACGAAGGCCGGGTGGTGCGCGAAAGCATTGGCGCAATCAAGCGTGCTGGTGCAGACCTGATCCTGACCTACTTCGCCATGGACCTGGCGCGCGAAGGTATCTGAAGCCAGGCCTTGGTCATGAAAGCCGCTGCCATTGCAGCGGCTTTTCTTTGTGCGCCGTTGGGTGGTGCCGCAAACGCACAAAAAAACAGTAAGCGCTGAATTATTTAGTGTCCTGTTGTATCTGATCCCTTAGAGCATTCATCGACAGGGATACGGGTATGAAACCTC > NC_002947/3313730‑3314006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |