| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,692,662 | T→A | 21.0% | Q336L (CAG→CTG) | PP_3259 ← | acyl‑CoA dehydrogenase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,692,662 | 0 | T | A | 21.0% | 35.3 / 5.5 | 19 | Q336L (CAG→CTG) | PP_3259 | acyl‑CoA dehydrogenase‑related protein |
| Reads supporting (aligned to +/- strand): ref base T (5/10); new base A (2/2); total (7/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
CCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTAC > NC_002947/3692515‑3692807 | ccccGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAgctgc < 2:284635/150‑1 (MQ=255) gCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAgt < 2:500982/150‑2 (MQ=255) cacgGTTGGGGTGAGTCGCGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAATGCGTGCCGCCTGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCTGCGCGCAACTGGGCCAGCTGCTGGCCCAGCa < 2:251698/150‑1 (MQ=255) caGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATc < 1:513322/150‑1 (MQ=255) ccAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAgtt < 2:333982/130‑3 (MQ=255) ccAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAgtt > 1:333982/1‑128 (MQ=255) aCGCGCCAGGTGCGCGCTTTGCTGCCATGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGa < 2:239636/150‑1 (MQ=255) aCGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCA‑CATTGCCTAGATCACGCTCGGCGTGGCTCTGa < 1:478736/150‑1 (MQ=255) gcgcTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCg > 2:525833/1‑150 (MQ=255) tgctggCAGGCTGCCCAAGGCGGGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCTTCTGGCAACTGGGCCAGCTGCTGGCCGAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGt < 2:176803/150‑1 (MQ=255) cccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCg > 1:429104/1‑150 (MQ=255) cgcccgcGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGAct < 1:122126/150‑1 (MQ=255) cccgcGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGActgc < 1:234430/150‑1 (MQ=255) gACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGAc < 2:208154/150‑1 (MQ=255) gATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAAc > 1:46380/1‑150 (MQ=255) ggTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGt < 1:222082/150‑1 (MQ=255) ccGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTgg > 2:402039/1‑150 (MQ=255) ggggCCAGCAGCTGGCCCCGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGc > 2:453368/2‑150 (MQ=255) cagcagCTGGCCCAGCATCGCC‑ATATGACGCCCGGGGTGGCTCTGACGCTGTAACACCGACAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTAc > 2:180826/1‑150 (MQ=255) cAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGccc > 2:191378/1‑113 (MQ=255) cAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGccc < 1:191378/113‑1 (MQ=255) | CCCCGGCGGGCACAGTTGGCGTGAGTCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCC‑AGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGACCTGCTCTAC > NC_002947/3692515‑3692807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |