| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,520,498 | A→T | 29.2% | intergenic (+45/+27) | cspD → / ← icd | DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,520,498 | 0 | A | T | 29.2% | 29.4 / 15.3 | 24 | intergenic (+45/+27) | cspD/icd | DNA replication inhibitor/NADP(+)‑specific isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base T (4/3); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT > NC_002947/4520354‑4520644 | cAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAaaga > 1:399370/1‑150 (MQ=255) ggCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCg > 1:145935/1‑150 (MQ=255) cACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTT‑TAAAGAAGCggc > 2:562800/1‑144 (MQ=255) cACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGTCGCTTCTTT‑TAAAGAAGCggc < 1:562800/146‑3 (MQ=255) tCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑tt > 1:270571/1‑150 (MQ=255) aTGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACAtgt < 1:99524/150‑1 (MQ=255) cAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCTCCAAAGCCTGa < 2:305411/150‑1 (MQ=255) cAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGa < 2:270571/150‑1 (MQ=255) cTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGa < 2:172084/92‑1 (MQ=255) cTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGa > 1:172084/1‑92 (MQ=255) cTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAgctgct < 2:399370/150‑1 (MQ=255) gCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGgc > 1:119250/1‑150 (MQ=255) cTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGaa < 2:145935/150‑1 (MQ=255) aCTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTTCACACGCAG‑GAGC‑TTCTGAGCCAGCCGTTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATc < 1:380690/150‑1 (MQ=255) cggtcGCTTCTTT‑TAAAGAAGCGGCCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGcc < 2:473028/133‑1 (MQ=255) cGGTCGCTTCTTT‑TAAAGAAGCGGCCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGcc > 1:473028/1‑137 (MQ=255) ggtcGCTTCTTT‑TAAAGAAGCGGCCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTgg > 1:372116/4‑134 (MQ=255) ggtcGCTTCTTT‑TAAAGAAGCGGCCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTgg < 2:372116/131‑1 (MQ=255) gtcGCTTCTTT‑TAAAGAAGCGGCCGATTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTg > 2:539976/3‑150 (MQ=255) gCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGGTGCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTg > 1:469525/1‑150 (MQ=255) ccGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTAc > 2:146859/1‑82 (MQ=255) ccGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTAc < 1:146859/82‑1 (MQ=255) ttt‑AAAAGTAGCGACCGGTTTTCTGTTGAC‑TTACATGTGTTTGATAATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTTTATCAATCGCTCGAAATCGTAGGTCACGGTCTTGACCGCAATGGCGCCATTGGTGCCGtt > 2:217535/1‑150 (MQ=255) ttt‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCtt > 1:344365/1‑150 (MQ=255) | CAAGGGCTTGCACGCCGTTGAAATCAAAGATGCCGCGGCCGCTGCGGCAGAGGCTGCCAGAGCCTTGGGCGAGCCGCAAAGCGAAACTGCAAAAGCCTGACTGCCTGCGCTGTGCCGATAGACAAAAAAATCGGCCGCTTCTTT‑AAAAGAAGCGACCGGTTTTCTGTTGCC‑TTACATGTGTTTGATCATTTCATCGCCAAAGCCTGAGCTGCTTACCAGCTTGGCGCCCTCCATCAATCGCTCGAAATCGTAGGTCACGGTCTTGGCCGCAATGGCGCCATTGGTGCCCTT > NC_002947/4520354‑4520644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |