Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,674,232 C→T 14.7% R320C (CGC→TGC)  PP_4137 → outer membrane siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,674,2320CT14.7% 62.9 / 4.2 30R320C (CGC→TGC) PP_4137outer membrane siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (15/9);  new base T (2/2);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

AACTATCTGGCACGCGACGACAGCCGCCCGCAACCAGGCAGCACCTGGGTGGGCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAATTTACCCGCAAT  >  NC_002947/4674085‑4674378
                                                                                                                                                   |                                                                                                                                                  
aaCTATCTGGCACGCGACGACAGCCGCCCGCAACCAGGCAGCACCTGGGTGGGCAACAAGACCACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATgcgc                                                                                                                                                  <  1:520701/150‑1 (MQ=255)
                                    ggCAGCACCTGGGTGGGCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCTGCCTGGTCTATCACGACTACCCGATGGACCTACGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTg                                                                                                              >  2:39191/1‑150 (MQ=255)
                                            cTGGGTGGGCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTAttt                                                                                                      >  2:310899/1‑150 (MQ=255)
                                              gggtgggCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTcc                                                                                                    <  2:594881/150‑1 (MQ=255)
                                                   ggCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCcgccgc                                                                                               <  1:310899/150‑1 (MQ=255)
                                                    gCAACAAGACGACCTTCTACCTCGAAGACGAGGGACGCCTGGAAGCCGGCCTGGTCTATCACGACTAGCCGATGGACCTGCGCGAAGGCCACATGGGCCTGAAGGTAGCCTGTACCGATGTCAGCGGCACGCTGAACTATTTctgccgag                                                                                              >  1:236329/1‑148 (MQ=255)
                                                     caacaaGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGa                                                                                             >  1:184620/1‑150 (MQ=255)
                                                           gacgacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATAc                                                                                                                                 <  1:171705/108‑1 (MQ=255)
                                                           gacgacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATAc                                                                                                                                 >  2:171705/1‑108 (MQ=255)
                                                             cgacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCg                                                                                                                                        <  1:113501/99‑1 (MQ=255)
                                                             cgacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCg                                                                                                                                        >  2:113501/1‑99 (MQ=255)
                                                              gacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGTGCCTGAAGGTCGCCtata                                                                                                                                  >  1:564940/1‑104 (MQ=255)
                                                              gacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGTGCCTGAAGGTCGCCtata                                                                                                                                  <  2:564940/104‑1 (MQ=255)
                                                              gacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGTGCCTGAAGGTCGCCtata                                                                                                                                  <  2:564862/104‑1 (MQ=255)
                                                              gacCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGTGCCTGAAGGTCGCCtata                                                                                                                                  >  1:564862/1‑104 (MQ=255)
                                                               acCTTCTACCTCGAAGACGAGGCACGCCTGGAAGTCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTc                                                                                   <  1:104185/150‑1 (MQ=255)
                                                                 cTTCTACCTCGAAGACGAGGCACGCCTGGGAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCgg                                                                                 >  2:290645/1‑150 (MQ=255)
                                                                 cTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGACCAAAAGGACAGAAGGTCGCCTATACCGATGTAAGCGGAAGGATGAACCAGTGCTGGCGGGACACACTGTTCgg                                                                                 >  1:261653/1‑150 (MQ=255)
                                                                     tACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCcgc                                                                             <  1:582848/150‑1 (MQ=255)
                                                                         tCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGa                                                                                                             >  1:591675/1‑114 (MQ=255)
                                                                         tCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGa                                                                                                             <  2:591675/114‑1 (MQ=255)
                                                                                                                aCGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGtt                                                       >  1:496331/1‑129 (MQ=255)
                                                                                                                aCGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGtt                                                       <  2:496331/129‑1 (MQ=255)
                                                                                                                           aTGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAgcggc                       >  1:248720/1‑150 (MQ=255)
                                                                                                                                 cTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTGTACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCg                 >  1:370126/1‑150 (MQ=255)
                                                                                                                                  tGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACAGTCGGTTGGCGTACCACCAAg                                         >  1:71057/1‑125 (MQ=255)
                                                                                                                                  tGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACAGTCGGTTGGCGTACCACCAAg                                         <  2:71057/125‑1 (MQ=255)
                                                                                                                                       gAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAAttt           >  1:285300/1‑150 (MQ=255)
                                                                                                                                         aGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCACGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAATTTAc         >  2:266233/1‑150 (MQ=255)
                                                                                                                                               cATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAATTTACCCGCaa   >  1:305154/1‑150 (MQ=255)
                                                                                                                                                aTGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAATTTACCCGCAAt  >  1:493006/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACTATCTGGCACGCGACGACAGCCGCCCGCAACCAGGCAGCACCTGGGTGGGCAACAAGACGACCTTCTACCTCGAAGACGAGGCACGCCTGGAAGCCGGCCTGGTCTATCACGACTACCCGATGGACCTGCGCGAAGGCCCCATGCGCCTGAAGGTCGCCTATACCGATGTCAGCGGCACGCTGAACTATTTCCGCCGCGACACACTGTTCGGCCGCGAGAGCAAGACCACCGTCGGTTGGCGTACCACCAAGCACCTGCCCAACAGCGGCGCTTCGCAATTTACCCGCAAT  >  NC_002947/4674085‑4674378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑