Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,716,693 G→C 10.8% Q626H (CAG→CAC)  PP_4173 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,716,6930GC10.8% 105.9 / 5.4 37Q626H (CAG→CAC) PP_4173two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base G (11/22);  new base C (2/2);  total (13/24)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

GACGCATCCGCCAGTTGCCCAACGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCAGT  >  NC_002947/4716548‑4716808
                                                                                                                                                  |                                                                                                                   
gACGCATCCGCCAGTTGCCCACCGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGtgc                                                                                                                 <  1:365206/150‑1 (MQ=255)
 aCGCATCCGCCAGTTGCCCAACGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGtgct                                                                                                                >  1:537215/1‑150 (MQ=255)
     aTCCGCCAGTTGCCCAACGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCACCTGCTGCaa                                                                                                            <  1:176392/150‑2 (MQ=255)
         gCCAGTTGCCCAACGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCt                                                                                                        <  2:352806/150‑1 (MQ=255)
                    aaCGGCGCCGGGCTACCGATCATCCCCCAGACAGCCTAACAC‑TCCCAAGTCGACCGCGAACGTTGCATGGCCACCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGt                                                                                             <  1:444545/150‑1 (MQ=255)
                        gcgcCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGa                                                                                         <  1:526344/150‑1 (MQ=255)
                              ggCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACcg                                                                                   >  1:210237/1‑150 (MQ=255)
                               gCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACcgc                                                                                  <  2:537215/150‑1 (MQ=255)
                                   ttcgcgatcGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACt                                                                              >  1:127321/7‑150 (MQ=255)
                                   ccGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACt                                                                              >  1:142155/1‑150 (MQ=255)
                                    cGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTg                                                                             <  1:569153/150‑1 (MQ=255)
                                     gATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTgg                                                                            >  1:565873/1‑150 (MQ=255)
                                        catcGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGa                                                                         <  2:12149/150‑1 (MQ=255)
                                           cGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGTTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATaa                                                                      <  1:12089/150‑1 (MQ=255)
                                             cccTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAAt                                                                    >  2:423678/1‑150 (MQ=255)
                                              ccTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATg                                                                   <  2:210237/150‑1 (MQ=255)
                                                       c‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGc                                                          <  1:314384/150‑1 (MQ=255)
                                                         aaCGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCg                                                         <  1:423678/150‑1 (MQ=255)
                                                                              gAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGa                                       <  1:355172/147‑1 (MQ=255)
                                                                              gAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGa                                       >  2:355172/1‑147 (MQ=255)
                                                                                  gTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCACctgctg                                                                                                               >  2:471599/1‑71 (MQ=255)
                                                                                           ccgccgGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGa                       >  2:19325/1‑150 (MQ=255)
                                                                                           ccgccgGTATGGACGATTACCTGAGCAAACCATTGAGACGCACCCACTTGCAGCCGCTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGa                       <  2:377220/150‑1 (MQ=255)
                                                                                            cgccgGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAACGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGAc                      <  2:523960/150‑1 (MQ=255)
                                                                                                          aTTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGt        <  2:127321/150‑1 (MQ=255)
                                                                                                              ccTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCa    <  2:565873/150‑1 (MQ=255)
                                                                                                              ccTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCa    <  1:278864/150‑1 (MQ=255)
                                                                                                              ccTGAGCAAACCCTTGCGATGTGCCGACTTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCATTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCa    <  1:189035/150‑1 (MQ=255)
                                                                                                               cTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCgg                                                        <  2:254378/97‑1 (MQ=255)
                                                                                                               cTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCgg                                                        >  1:254378/1‑97 (MQ=255)
                                                                                                               cTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCAg   <  2:344274/150‑1 (MQ=255)
                                                                                                                           ttGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATg                                                                   >  1:557037/1‑74 (MQ=255)
                                                                                                                           ttGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATg                                                                   <  2:557037/74‑1 (MQ=255)
                                                                                                                                      cGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGAc          >  1:251992/1‑120 (MQ=255)
                                                                                                                                      cGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGAc          <  2:251992/120‑1 (MQ=255)
                                                                                                                                       gcgctGCAGCACCTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCAGt  <  2:338633/123‑1 (MQ=255)
                                                                                                                                       gcgctGCAGCACCTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCAGt  >  1:338633/5‑127 (MQ=255)
                                                                                                                                                  |                                                                                                                   
GACGCATCCGCCAGTTGCCCAACGGCGCCGGGCTACCGATCATCGCCCTGACCGCC‑AACGCGTTGCAGGGCGACCGCGAACGTTGCATGGCCGCCGGTATGGACGATTACCTGAGCAAACCCTTGCGCCGCACCGAATTGCAGCAGGTGCTGCAGCGCTGGTTACCCGGTCAGACAACCGCGACTGGCGATAAATGCTAAAGTGCGGCAGTCTTAAACACTGGACAGGACCTTCATCGGACCTGAAAATAGACGTTTCAGT  >  NC_002947/4716548‑4716808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑