Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,770,357 G→A 10.5% V2985V (GTC→GTT)  pvdD ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,770,3570GA10.5% 99.1 / 2.2 38V2985V (GTC→GTT) pvdDnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (14/20);  new base A (2/2);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

GCGCCTCGATTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCATGACA  >  NC_002947/4770209‑4770477
                                                                                                                                                    |                                                                                                                        
gcgcCTCGATTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGa                                                                                                                         >  1:578947/1‑150 (MQ=255)
    cTCGATTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACaaa                                                                                                                     <  2:64522/150‑1 (MQ=255)
     tCGATTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACaaaa                                                                                                                    >  1:293116/1‑149 (MQ=255)
       gATTTCGCCGAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGACGCACCGGGTCGCCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCg                                                                                                                  <  2:392196/150‑1 (MQ=255)
        aTTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGt                                                                                                                 <  2:274398/150‑1 (MQ=255)
        aTTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCAACAAAGCGt                                                                                                                 >  1:488349/1‑150 (MQ=255)
        aTTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGAAAACAAAGCGt                                                                                                                 >  1:488381/1‑150 (MQ=255)
          ttCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTc                                                                                                               <  1:418778/150‑1 (MQ=255)
            cGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTcgg                                                                                                             <  2:270580/150‑1 (MQ=255)
             gCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTcggc                                                                                                            >  2:293835/1‑150 (MQ=255)
              cccAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGCAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTcggcg                                                                                                           >  2:93751/1‑150 (MQ=255)
                     cGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGcc                                                                                                    <  1:293835/150‑1 (MQ=255)
                      gATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGccc                                                                                                   >  2:544685/1‑150 (MQ=255)
                                ccGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTc                                                                                         <  2:340210/150‑1 (MQ=255)
                                  gcgAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAg                                                                                       <  1:307633/150‑1 (MQ=255)
                                  gcgAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAg                                                                                       <  1:109690/150‑1 (MQ=255)
                                    gAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGt                                                                                     <  1:494895/150‑1 (MQ=255)
                                            aCCTGATTGTCCAGGCGCCCGAGGTAAGCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACACGACGGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCgg                                                                             <  1:195593/150‑1 (MQ=255)
                                             ccTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAg                                                                                       >  2:76192/1‑139 (MQ=255)
                                             ccTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAg                                                                                       <  1:76192/139‑1 (MQ=255)
                                                gATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCa                                                                         >  1:36586/1‑150 (MQ=255)
                                                        aGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCACGGGCCCCGCCGGtg                                                                 >  2:506147/1‑149 (MQ=255)
                                                             cccGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGcc                                                            >  1:167245/1‑150 (MQ=255)
                                                                    tAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGt                                                                                           <  2:541571/112‑1 (MQ=255)
                                                                    tAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGt                                                                                           >  1:541571/1‑112 (MQ=255)
                                                                     aaTCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGAGGTACGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTaga                                                    <  1:506147/150‑1 (MQ=255)
                                                                      aTCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTagag                                                   <  2:91457/150‑1 (MQ=255)
                                                                      aTCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGGAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTGCCGCCCAGGTagaa                                                   >  2:57230/1‑149 (MQ=255)
                                                                                ccGTCCTCCCGCTGGCGGATCAGGTGACCGGGACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCTGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGcc                                         >  2:95374/1‑150 (MQ=255)
                                                                                                  aCCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTggg                       <  1:93751/150‑1 (MQ=255)
                                                                                                             ggtacggtacAGGCGCCCACCCGGGTTGCCTGGGTCGGCAACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAAc            <  2:488349/150‑1 (MQ=255)
                                                                                                             ggtacggtacAGGCGCCCACCCGGGTTGCCTGGGTCGGCAACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAAc            <  2:488381/150‑1 (MQ=255)
                                                                                                                  ggtacAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCa       <  2:36586/150‑1 (MQ=255)
                                                                                                                   gtacAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCAt      >  1:40466/1‑150 (MQ=255)
                                                                                                                     acAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCATGa    <  2:578947/150‑1 (MQ=255)
                                                                                                                       aGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCATGACa  <  1:544685/150‑1 (MQ=255)
                                                                                                                                     gTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAg                  >  2:470572/1‑120 (MQ=255)
                                                                                                                                     gTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAg                  <  1:470572/120‑1 (MQ=255)
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GCGCCTCGATTTCGCCCAGTTCGATGCGGAAACCGCGAACCTTGACCTGATTGTCCAGGCGCCCGAGGTAATCGAAGGTGCCGTCCTCCCGCTGGCGCACCAGGTCACCGGTACGGTACAGGCGCCCACCCGGGTTGCCTGGGTCGGCGACAAAGCGTTCGGCGGTAAGCCCCGGGCGGTTCAGGTAGCCGCGGGCCACGCCGGTACCGCCCAGGTAGAGTTCTCCCGCCACACCGGGCGGCAGTGGGTTCAGGTCAACGTCCATGACA  >  NC_002947/4770209‑4770477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑