| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,827,099 | C→A | 11.6% | S1379I (AGT→ATT) ‡ | pvdL ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,827,099 | 0 | C | A | 11.6% | 120.8 / 6.6 | 43 | S1379I (AGT→ATT) ‡ | pvdL | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base C (19/19); new base A (3/2); total (22/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.42e-01 | |||||||||||
TGACGTTGATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCACCAGCTTGCAGCCGGTGACCAG > NC_002947/4826962‑4827247 | tGACGTTGATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCt > 2:259420/1‑150 (MQ=255) gTTGATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCATCACGCGGTCACGCCGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCGCTTGCCTGCGGCTCCTg < 2:94270/150‑1 (MQ=255) gTTGATGGCGGTTTCAGTCGGGCCATAACGGATGTGCAGTAGCACCTGAGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTg > 2:434469/1‑150 (MQ=255) tGATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGa < 2:253880/150‑1 (MQ=255) gATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTccctgat < 2:429389/150‑6 (MQ=255) gCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAac < 1:290795/150‑1 (MQ=255) ggTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCt > 2:272354/1‑140 (MQ=255) ggTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCt < 1:272354/140‑1 (MQ=255) ggTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAacac < 1:30689/150‑1 (MQ=255) gTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAAc > 1:549119/1‑150 (MQ=255) gTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAAc > 1:303859/1‑150 (MQ=255) gggCCACACCGGTTGCGCAGTTGCACCTGCGGTTGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAgc < 2:497629/150‑1 (MQ=255) aTACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACa > 1:142472/1‑143 (MQ=255) aTACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACa < 2:142472/143‑1 (MQ=255) ttGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCTAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAacac < 1:110751/128‑1 (MQ=255) ttGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAacac > 2:110751/1‑128 (MQ=255) gTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCa < 2:592086/147‑1 (MQ=255) gTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCa > 1:592086/1‑147 (MQ=255) gTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCAAGTGCCTGcagta > 2:572696/1‑104 (MQ=255) gTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCAAGTGCCTGcagta < 1:572696/108‑5 (MQ=255) gCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCAccc < 2:591485/150‑1 (MQ=255) caCCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGc > 1:237847/1‑150 (MQ=255) ggTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGc < 2:256917/148‑1 (MQ=255) ggTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGc > 1:256917/1‑148 (MQ=255) aaCGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGa < 2:601239/150‑1 (MQ=255) ttCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACACCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTc > 1:453590/1‑150 (MQ=255) gCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCAcc > 1:9923/1‑150 (MQ=255) gagaACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCGACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGcc > 1:407458/1‑102 (MQ=255) gagaACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCGACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGcc < 2:407458/102‑1 (MQ=255) gaACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCTTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGc < 1:12449/150‑1 (MQ=255) ccAGCGCAAGCTACTGCAGTCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAACGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAACCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCAc > 2:1645/1‑150 (MQ=255) cAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTcgc > 1:170359/1‑124 (MQ=255) cAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTcgc < 2:170359/124‑1 (MQ=255) aGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCgg > 2:111163/1‑118 (MQ=255) aGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCgg < 1:111163/118‑1 (MQ=255) gagccgCAGGCAAGTGCCTGCAGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCACCAGCTTGCAg > 1:475046/6‑150 (MQ=255) ccgCAGGCAAGTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAg > 2:450399/3‑62 (MQ=38) ccgCAGGCAAGTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAg < 1:450399/60‑1 (MQ=38) cTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCAc > 2:398902/1‑121 (MQ=255) cTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCAc < 1:398902/121‑1 (MQ=255) tGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGCACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGGACCAGCTTGCAGCCgg > 2:568897/1‑150 (MQ=255) gCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCACCAGCTTGCAGCCGGt < 2:549119/150‑1 (MQ=255) aCTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCACCAGCTTGCAGCCGGTGACCAg > 2:235305/1‑150 (MQ=255) | TGACGTTGATGGCGGTTTCAGTCGGGCCATACCGGTTGTGCAGTTGCACCTGCGGCAGCACCTGCAGCACGCGGTCACGCAGCGTGGCGGACAACGCTTCGCCACCCGAGAACACCCAGCGCAAGCTACTGCAGGCACTTGCCTGCGGCTCCTGGACGAACACCTGCAACAGCGGCGGTACAAAGTGCAGCGTAGTCACCCCATAAGCCTGCACCAGGGCAGCGATACGCTGCGGGTCGCGATGTTCACCAGGGCCGGCGAGCACCAGCTTGCAGCCGGTGACCAG > NC_002947/4826962‑4827247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |