Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,120,102 G→T 13.3% H68N (CAC→AAC)  PP_4506 ← nitroreductase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,120,1020GT13.3% 78.6 / 4.1 30H68N (CAC→AAC) PP_4506nitroreductase family protein
Reads supporting (aligned to +/- strand):  ref base G (12/14);  new base T (2/2);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

CGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTCGCGCTGGGCGGCATTGGGCGCCGGGT  >  NC_002947/5119959‑5120251
                                                                                                                                               |                                                                                                                                                     
cgcACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCAc                                                                                                                                                 <  1:120909/150‑1 (MQ=255)
     aGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCtt                                                                                                                                            <  2:599744/150‑1 (MQ=255)
             gccAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAAc                                                                                                                                    <  2:302018/150‑1 (MQ=255)
                aGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCg                                                                                                                                          >  2:442941/1‑141 (MQ=255)
                aGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCg                                                                                                                                          <  1:442941/141‑1 (MQ=255)
                                               ggTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGa                                                                                                  <  2:549474/150‑1 (MQ=255)
                                                gTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGTGCCTTGTCCAGTGCAGCCTGGCTGTCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGAt                                                                                                 >  1:64998/1‑150 (MQ=255)
                                                gTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGAt                                                                                                 <  1:115450/150‑1 (MQ=255)
                                                 tCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATg                                                                                                >  1:539917/1‑150 (MQ=255)
                                                      cAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAg                                                                                           >  2:591685/1‑150 (MQ=255)
                                                                accaccacGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCAc                                                                                 >  2:337618/1‑150 (MQ=255)
                                                                  caccacGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACgg                                                                               >  1:525481/1‑150 (MQ=255)
                                                                         aTCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGc                                                                        >  2:539314/1‑150 (MQ=255)
                                                                          tCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTAGAGTTTGAGAAAACGCCACGGCCGCAGCt                                                                       >  1:157195/1‑150 (MQ=255)
                                                                               aaCGGCGCCCGCAGCGGCACCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTc                                                                                                                                           >  2:543574/1‑77 (MQ=255)
                                                                               aaCGGCGCCCGCAGCGGCACCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTc                                                                                                                                           <  1:543574/77‑1 (MQ=255)
                                                                                  ggCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGa                                                               <  1:30643/150‑1 (MQ=255)
                                                                                       ccGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCgg                                                          >  1:68265/1‑150 (MQ=255)
                                                                                               gCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTTCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCg                                                                  <  1:415319/134‑1 (MQ=255)
                                                                                               gCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTTCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCg                                                                  >  2:415319/1‑134 (MQ=255)
                                                                                               gCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTTCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCg                                                                  >  2:415302/1‑134 (MQ=255)
                                                                                               gCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTTCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCg                                                                  <  1:415302/134‑1 (MQ=255)
                                                                                                  tCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAggg                                               >  2:197733/1‑150 (MQ=255)
                                                                                                           ccTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGTACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGa                                      >  1:250968/1‑150 (MQ=255)
                                                                                                            cTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGaa                                     <  1:441238/150‑1 (MQ=255)
                                                                                                                 ccAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAgcg                                <  2:539917/150‑1 (MQ=255)
                                                                                                                     tGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCCCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCTTc                            <  1:539314/150‑1 (MQ=255)
                                                                                                                      gCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTcg                           <  1:552313/150‑1 (MQ=255)
                                                                                                                                      cGCCCTTGTGCTGCACGGCTTCGCCGAACACCTCACCCTGCCTCTCGCGCCCCTGGCCTTCGATGGTAACAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTCGCGCTGGGCGGCATTgg           <  1:502823/150‑1 (MQ=255)
                                                                                                                                       gCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTCGCGCTGGGCGGCATTggg          <  2:246086/150‑1 (MQ=255)
                                                                                                                                               gctgcACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTCGCGCTGGGCGGCATTGGGCGCCGggt  <  1:337618/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                     
CGCACAGCCCGCCGCCAGGCGCTGCTCGGACTTGGGCACCTTGAAGTGGTCCTGCAACCTGGCCACCACCACGATCAGCAACGGCGCCCGCAGCGGCATCGCACGGGCCTTGTCCAGTGCAGCCTGGCTGGCATCGCCCTTGTGCTGCACGGCTTCGGCGAACAGCTCACCCAGCTTCTCGCGGCCCTGGCCTTCGATGGTAAGAAAACGCCACGGCCGCAGCTGGCCGTGATCCGGGGCGCGCAGGGCAGCCTGGAACAGCGCCTCGCGCTGGGCGGCATTGGGCGCCGGGT  >  NC_002947/5119959‑5120251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑