| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,275,342 | T→G | 17.4% | E468A (GAG→GCG) | cioA ← | cyanide insensitive ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,275,342 | 0 | T | G | 17.4% | 39.5 / 6.3 | 23 | E468A (GAG→GCG) | cioA | cyanide insensitive ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base T (11/8); new base G (2/2); total (13/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.10e-01 | |||||||||||
GGAAGAGCATGCCGATCCCCAGGTCGAATCCGTCCATCACCACGTACATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTGCCAAACAGGGCGAA > NC_002947/5275202‑5275490 | ggAAGAGCATGCCGATCCCCAGGTCGAATCCGTCCATCACCACGTACATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCcat > 1:454851/1‑150 (MQ=255) cacGTACATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCAGCGGCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTg > 1:468634/1‑150 (MQ=255) aCATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTg < 2:454851/150‑1 (MQ=255) aCATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTg < 2:39991/150‑1 (MQ=255) gAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCGCGAGGcc < 1:111960/90‑1 (MQ=255) gAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCGCGAGGcc > 2:111960/1‑90 (MQ=255) atCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCa > 2:243954/1‑150 (MQ=255) cACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTGCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCAcc > 2:215580/1‑150 (MQ=255) ggCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTc > 2:142750/1‑150 (MQ=255) tCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCgg > 2:73142/1‑65 (MQ=255) tCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCgg < 1:73142/65‑1 (MQ=255) gAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGcc < 1:142750/150‑1 (MQ=255) tGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGgc < 1:243954/150‑1 (MQ=255) ttCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGca > 2:603826/1‑150 (MQ=255) tCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGt < 1:364136/150‑1 (MQ=255) gTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAg > 1:319376/1‑150 (MQ=255) ggCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCgg < 1:603826/150‑1 (MQ=255) cTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTg < 2:346266/150‑1 (MQ=255) cTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTg > 1:205129/1‑150 (MQ=255) gatGGCCGCGAGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGcatcat > 1:283029/3‑126 (MQ=255) gatGGCCGCGAGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGcatcat < 2:283029/124‑1 (MQ=255) cTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTGCCAAACAGGGCGaa > 1:363020/1‑150 (MQ=255) cTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTGCCAAACAGGGCGaa > 1:19470/1‑150 (MQ=255) | GGAAGAGCATGCCGATCCCCAGGTCGAATCCGTCCATCACCACGTACATCATGACGCCGAAGATGATGATCACGGCCCAGATCAGTGGAAGGTCGATACCCATGGTTCAGTTCCCCTCGTTCAGGCTGGCAGAGGTGGCCTCGCGGCCATCATCGGCGGCGGACAAAGGCCGTGCCGGCGTGCGTTTCTGGCCTGGGCCTCCCGGTGTGTGCTCGTCACCCTCACCGGTTTGCGGCCCTTTGCGCACCAGGCGCATCATGTAGCCAAGGCCGGTGCCAAACAGGGCGAA > NC_002947/5275202‑5275490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |