Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,334,378 G→T 9.7% V254F (GTC→TTC)  PP_4695 → two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,334,3780GT 9.7% 120.9 / 2.8 41V254F (GTC→TTC) PP_4695two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (24/13);  new base T (2/2);  total (26/15)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CTTGTATGGCGTATTCGGTGGCCCGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTGGGCCGGCCTGCGCCTGGGCGCCA  >  NC_002947/5334239‑5334525
                                                                                                                                           |                                                                                                                                                   
cTTGTATGGCGTATTCGGTGGCCCGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCTTCATGCCGca                                                                                                                                           >  2:367206/1‑150 (MQ=255)
cTTGTATGGCGTATTCGGTGGCCCGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCTTCATGCCGca                                                                                                                                           >  2:367105/1‑150 (MQ=255)
            aTTCGGTGGCCCGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACAt                                                                                                                               >  1:13213/1‑150 (MQ=255)
                 gTGGCCCGCACGAGCTCGAAGTCCGCCTGCTCCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCt                                                                                                                          <  2:604381/150‑1 (MQ=255)
                      ccGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTAcc                                                                                                                     >  1:2165/1‑150 (MQ=255)
                         cACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCgag                                                                                                                  <  1:102072/150‑1 (MQ=255)
                              gCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCATAGGTACCACATGGCCTTTACCGAGAACCt                                                                                                             <  1:173734/150‑1 (MQ=255)
                                        tGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCg                                                                                                                                             >  2:494063/1‑108 (MQ=255)
                                        tGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCg                                                                                                                                             <  1:494063/108‑1 (MQ=255)
                                          gctgctGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGcgctc                                                                                                 >  1:18499/1‑150 (MQ=255)
                                                gcAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGt                                                                                           >  1:156464/1‑150 (MQ=255)
                                                      ccAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAgcgc                                                                                     >  1:64200/1‑150 (MQ=255)
                                                       cAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGcc                                                                                    >  1:31683/1‑150 (MQ=255)
                                                            cGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGaa                                                                                                                >  1:20504/1‑117 (MQ=255)
                                                            cGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGaa                                                                                                                <  2:20504/117‑1 (MQ=255)
                                                            cGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTg                                                                               >  2:560076/1‑150 (MQ=255)
                                                              cccTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCTTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTggg                                                                             <  1:367206/150‑1 (MQ=255)
                                                              cccTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCTTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTggg                                                                             <  1:367105/150‑1 (MQ=255)
                                                                 tCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGcc                                                                          >  2:268421/1‑150 (MQ=255)
                                                                 tCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGcc                                                                          >  1:18988/1‑150 (MQ=255)
                                                                      gccCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCg                                                                     >  1:3752/1‑150 (MQ=255)
                                                                         cTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTg                                                                  >  1:254613/1‑150 (MQ=255)
                                                                                 cccACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCctgct                                                          >  1:81520/1‑150 (MQ=255)
                                                                                 cccACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCctgct                                                          >  1:73454/1‑150 (MQ=255)
                                                                                       gCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCtgat                                                    <  2:18988/150‑1 (MQ=255)
                                                                                           gAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGc                                                >  1:589688/1‑150 (MQ=255)
                                                                                             aGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCGCGCTGGTCCGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGCTGAGCCt                                              >  2:148843/1‑150 (MQ=255)
                                                                                                 ccATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCgaga                                                                                                                 >  1:563408/1‑79 (MQ=255)
                                                                                                 ccATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCgaga                                                                                                                 <  2:563408/79‑1 (MQ=255)
                                                                                                          aCCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTg                                 <  2:18499/150‑1 (MQ=255)
                                                                                                          aCCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTg                                 <  1:268421/150‑1 (MQ=255)
                                                                                                            ccTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGt                               >  1:306345/1‑150 (MQ=255)
                                                                                                                   ctgGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTgg                        <  1:413134/150‑1 (MQ=255)
                                                                                                                        gtTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCtga                                                     <  1:508963/116‑1 (MQ=255)
                                                                                                                        gtTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCtga                                                     >  2:508963/1‑116 (MQ=255)
                                                                                                                             tcGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTGGGCCGGCCTgc              >  1:101135/1‑150 (MQ=255)
                                                                                                                                  tCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTGGGCCGGCCTGCGCCTg         >  1:305054/1‑150 (MQ=255)
                                                                                                                                      ccATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCCGCGCCGGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTCCCGTGCCGCTGGTGCTGTGGGCCGGCCTGCGCCTGGgcg     >  2:41728/1‑150 (MQ=255)
                                                                                                                                        aTCGTCATGCCGCACATGTACCACATGGCCTTTAcc                                                                                                                     <  1:50587/36‑1 (MQ=255)
                                                                                                                                        aTCGTCATGCCGCACATGTACCACATGGCCTTTAcc                                                                                                                     >  2:50587/1‑36 (MQ=255)
                                                                                                                                         tCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTGGGCCGGCCTGCGCCTGGGCGCCa  <  1:270727/150‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                   
CTTGTATGGCGTATTCGGTGGCCCGCACGAGCTGGAAGTCTGGCTGCTGCAGAACCAGACCGCCCTCGCCGCCCTGCACACCCCACTGCAGGAAGGCCCATGGCGCACCCTGCTGCTGGTGTTCTTCGCCTCGGCCATCGTCATGCCGCACATGTACCACATGGCCTTTACCGAGAACCTCAACCCGCGCTCGCTGGTCAGCGCCAGCTGGGGCCTGCCGCTGTTCCTGCTGCTGATGAGCCTTGCCGTGCCGCTGGTGCTGTGGGCCGGCCTGCGCCTGGGCGCCA  >  NC_002947/5334239‑5334525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑