| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,564,387 | C→A | 100% | V62L (GTA→TTA) | hfq ← | RNA‑binding protein Hfq |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,564,387 | 0 | C | A | 92.0% | 66.0 / ‑1.5 | 25 | V62L (GTA→TTA) | hfq | RNA‑binding protein Hfq |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (9/14); minor base G (1/1); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACCTTCCAAGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTACGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTGACCAGGTAAATGGATACCGGGACCTTTTCTTTTCTCAAG > NC_002947/5564240‑5564529 | gACCTTCCAAGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGTTTGGCAGGCGGACCGGACGGGCCGGAACTAAgg < 1:426427/150‑1 (MQ=255) ttCCAAGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCga > 1:290540/1‑150 (MQ=255) ccAAGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCgaga < 1:593842/150‑1 (MQ=255) aGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATgg > 1:547774/1‑150 (MQ=255) gAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGt > 2:598181/1‑150 (MQ=255) aaCGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAGGGTCGAGATGGCGTGCTTGTAg > 2:494773/1‑150 (MQ=255) accaccGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAGGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGAc < 1:494773/150‑1 (MQ=255) accGTGGCGCTGAAAGAACAATGCAGGCTCCTATCAGACGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGgt < 1:241415/150‑1 (MQ=255) cgcTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGttcttc < 1:248278/150‑1 (MQ=255) aGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTg > 1:290555/1‑150 (MQ=255) gCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCa < 2:20139/132‑1 (MQ=255) gCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCa > 1:20139/1‑132 (MQ=255) cTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTc < 2:290540/150‑1 (MQ=255) cTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGaa < 1:598181/150‑1 (MQ=255) tCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGaa > 1:597654/1‑131 (MQ=255) tCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGaa < 2:597654/131‑1 (MQ=255) ccGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACTACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATa < 2:547774/150‑1 (MQ=255) ccGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATa < 2:290555/150‑1 (MQ=255) gCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGt < 1:368132/150‑1 (MQ=255) tCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTg > 1:222511/1‑150 (MQ=255) cAGGCGGACCGGACGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTCGAACATCTCGCTGATGTTCTTTGGCAGCAATACCACGAACAGGGCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTGACCAGGTAAATGGATACCgg < 1:141365/150‑1 (MQ=255) accggacGGGCCGGAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTGACCAGGTAAATGGATACCGGGACcttt < 1:123844/150‑1 (MQ=255) ggAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATtcgatcga > 2:295680/1‑89 (MQ=255) ggAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATtcgatcga < 1:295680/89‑1 (MQ=255) ggAACTAAGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTGACCAGGTAAATGGATACCGGGACCTTTTCTTTTCTCAAg > 2:187921/1‑150 (MQ=255) | GACCTTCCAAGTGAACGAGCAACGCCCGCTCACCACCACCGTGGCGCTCAAAGAACAATGCAGGCTCCTATCAGGCGTTGCCTGGCTCGCTGTCGCCGTGCTCGCTGTCGGTCGGGCTTGGCAGGCGGACCGGACGGGCCGGAACTACGGTCGAGATGGCGTGCTTGTAGACCATCTGGCTGACGGTGTTCTTCAGCAATACCACGAACTGGTCGAACGATTCGATCGAGCCCTGCAGTTTGATACCGTTGACCAGGTAAATGGATACCGGGACCTTTTCTTTTCTCAAG > NC_002947/5564240‑5564529 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |